당귀와 감초 복합 한약추출물의 모발성장 촉진효과
The Promoting Effect of Angelica gigas Nakai and Glycyrrhiza uralensis Fischet Oriental Medicine Complex Extracts on Hair Growth
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This study was carried out to investigate the effect of Angelica gigas Nakai and Glycyrrhiza uralensis Fischet oriental medicine complex extracts on hair growth in a shaving animal model of C57BL/6 mice. Five weeks old male mice were acclimated for one week under 22±1..C room temperature, 50±5% relative humidity and 12 hours of a light/dark cycle before beginning the experiment. The animals were divided into 4 groups including normal group(saline, N), vehicle control group (jojoba oil+100% ethanol+saline, VC), positive control group (3% minoxidil, PC), and experimental group (oriental medicine complex extracts, E) and they were topically applied with an amount of 0.2 mL once a day, 6 days a week, for 3 weeks. E and PC groups showed a prominently promoted hair regrowth compared to the N or VC groups in gross observation. E and PC groups also promoted the development and elongation of hair follicles compared to the N or VC group. IGF-1 expression in the skin was significantly (p.0.001) increased in the PC and E groups compared to the N or VC group. Immunolocalization of SCF antigens was heavily stained in sebaceous gland, bulge and epidermis of the PC group, mildly stained in sebaceous gland and bulge of the E group, weakly stained in sebaceous gland of the VC group and little stained in the N group. These results indicate that Angelica gigas Nakai and Glycyrrhiza uralensis Fischet oriental medicine complex extracts effectively stimulated hair growth in an animal model and it can be used practically for hair growth or prevention of hair loss in human beings.
Abstract
This study was carried out to investigate the effect of Angelica gigas Nakai and Glycyrrhiza uralensis Fischet oriental medicine complex extracts on hair growth in a shaving animal model of C57BL/6 mice. Five weeks old male mice were acclimated for one week under 22±1..C room temperature, 50±5% relative humidity and 12 hours of a light/dark cycle before beginning the experiment. The animals were divided into 4 groups including normal group(saline, N), vehicle control group (jojoba oil+100% ethanol+saline, VC), positive control group (3% minoxidil, PC), and experimental group (oriental medicine complex extracts, E) and they were topically applied with an amount of 0.2 mL once a day, 6 days a week, for 3 weeks. E and PC groups showed a prominently promoted hair regrowth compared to the N or VC groups in gross observation. E and PC groups also promoted the development and elongation of hair follicles compared to the N or VC group. IGF-1 expression in the skin was significantly (p.0.001) increased in the PC and E groups compared to the N or VC group. Immunolocalization of SCF antigens was heavily stained in sebaceous gland, bulge and epidermis of the PC group, mildly stained in sebaceous gland and bulge of the E group, weakly stained in sebaceous gland of the VC group and little stained in the N group. These results indicate that Angelica gigas Nakai and Glycyrrhiza uralensis Fischet oriental medicine complex extracts effectively stimulated hair growth in an animal model and it can be used practically for hair growth or prevention of hair loss in human beings.
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