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The Changes in Hair Growth-Relevant Enzyme Activity and Cytokine Expression Induced by Topical Application of Sandalwood Oil to C57BL/6 Mice

The Changes of Hair Growth Relevant Enzyme Activity and Cytokine Expression by Topical Application of Sandalwood Oil to C57BL/6 Mice

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초록

This study was conducted to know the changes of hair growth relevant factors including enzyme activity and cytokine expression by topical application of sandalwood oil to C57BL/6 mice. C57BL/6 mice were randomized into three groups consisting of normal group (N: saline), positive control group (PC: 3% minoxidil), and experimental group (E: 3% Sandalwood oil). Sandalwood oil(SO) was topically applied to the backs of mice with 100μL a day, once a day, 5 days a week, for 4 weeks, and the changes of hair growth relevant factors in mice skin were observed during 16 weeks. The alkaline phosphatase(ALP) activity in the PC and E groups was significantly higher(p⁄0.001) than that of the N group. The mRNA expression of hair growth promoting factors including prolactin(PRL) and vascular endothelial growth factor (VEGF) in the PC and E groups significantly(p⁄0.001) increased, compared to the N group. The mRNA expression of transforming growth factor-β1 (TGF-β1), hair growth inhibitory factor, in the PC and E groups was significantly decreased(p⁄0.001), compared to the N group. These results suggest that changes in values of growth related factors including ALP activity and mRNA expression of PRL, VEGF, and TGF-β1 are useful markers to represent the hair growth cycle.

Abstract

This study was conducted to know the changes of hair growth relevant factors including enzyme activity and cytokine expression by topical application of sandalwood oil to C57BL/6 mice. C57BL/6 mice were randomized into three groups consisting of normal group (N: saline), positive control group (PC: 3% minoxidil), and experimental group (E: 3% Sandalwood oil). Sandalwood oil(SO) was topically applied to the backs of mice with 100μL a day, once a day, 5 days a week, for 4 weeks, and the changes of hair growth relevant factors in mice skin were observed during 16 weeks. The alkaline phosphatase(ALP) activity in the PC and E groups was significantly higher(p⁄0.001) than that of the N group. The mRNA expression of hair growth promoting factors including prolactin(PRL) and vascular endothelial growth factor (VEGF) in the PC and E groups significantly(p⁄0.001) increased, compared to the N group. The mRNA expression of transforming growth factor-β1 (TGF-β1), hair growth inhibitory factor, in the PC and E groups was significantly decreased(p⁄0.001), compared to the N group. These results suggest that changes in values of growth related factors including ALP activity and mRNA expression of PRL, VEGF, and TGF-β1 are useful markers to represent the hair growth cycle.

참고문헌 (26)

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