배양 인체섬유모세포에서 산화염모제 성분인 Hydrogen Peroxide의 세포독성에 대한 까마중 추출물의 항산화 방어효과
The Protective Antioxidative Effect of Solanum nigrum L. Extract against the Cytotoxicity of Hydrogen Peroxide in Cultured Human Dermal Fibroblasts
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To clarify the cytotoxicity and antioxidative effect of Solanum nigrum L. (SN) extract, cell adhesion activity and cell viability were analysed by XTT or MTT assay after human dermal fibroblasts (Detroit 551) were cultured in media containing various concentrations of H2O2. And also, the effect of antioxidant, butylated hydroxytoluene (BHT), against H2O2-induced cytotoxicity was examined. For the protective effect of SN extract on H2O2-induced cytotoxicity, Detroit 551 cells were pretreated with 40 or 60 μg/mL of SN extract for 2 hours. And also, the antioxidative effects of SN extract were measured by superoxide dismutase (SOD)-like activity and the inhibitory activity of lipid peroxidation. In this study, H2O2 remarkably decreased cell viability as well as cell adhesion activity in dose-dependent manner compared with control. And the XTT50 and MTT50 values were determined at 35 and 40 μM of H2O2, respectively. In the effect of antioxidant, BHT effectively prevented H2O2-induced cytotoxicity by the significant increase in cell adhesion activity. In the protective effect of SN extract on H2O2-induced cytotoxicity, SN extract remarkably increased cell adhesion activity which was decreased by H2O2-induced cytotoxicity, and also it showed both of SOD-like activity and the inhibitory activity of lipid peroxidation. From these results,it is suggested that the SN extract effectively prevented the cytotoxicity induced by an oxidant, H2O2 as component of oxidized hair dye via its antioxidative effect, and also it may be a putative resources for beauty as an alternative antioxidant in hair dye.
Abstract
To clarify the cytotoxicity and antioxidative effect of Solanum nigrum L. (SN) extract, cell adhesion activity and cell viability were analysed by XTT or MTT assay after human dermal fibroblasts (Detroit 551) were cultured in media containing various concentrations of H2O2. And also, the effect of antioxidant, butylated hydroxytoluene (BHT), against H2O2-induced cytotoxicity was examined. For the protective effect of SN extract on H2O2-induced cytotoxicity, Detroit 551 cells were pretreated with 40 or 60 μg/mL of SN extract for 2 hours. And also, the antioxidative effects of SN extract were measured by superoxide dismutase (SOD)-like activity and the inhibitory activity of lipid peroxidation. In this study, H2O2 remarkably decreased cell viability as well as cell adhesion activity in dose-dependent manner compared with control. And the XTT50 and MTT50 values were determined at 35 and 40 μM of H2O2, respectively. In the effect of antioxidant, BHT effectively prevented H2O2-induced cytotoxicity by the significant increase in cell adhesion activity. In the protective effect of SN extract on H2O2-induced cytotoxicity, SN extract remarkably increased cell adhesion activity which was decreased by H2O2-induced cytotoxicity, and also it showed both of SOD-like activity and the inhibitory activity of lipid peroxidation. From these results,it is suggested that the SN extract effectively prevented the cytotoxicity induced by an oxidant, H2O2 as component of oxidized hair dye via its antioxidative effect, and also it may be a putative resources for beauty as an alternative antioxidant in hair dye.
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