배양 SK-MEL-3세포에 있어서 로즈마리 추출물의 항산화능 및 멜라닌화에 대한 영향
Effect of Rosemary Extract on Antioxidative Activity and Melanogenesis in Cultured SK-MEL-3 Cells
0
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
To evaluate the effect of Rosemary(Rosemarinus officinalis) extract on antioxidative activity and melanogenesis on cultured SK-MEL-3, the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, tyrosinase activity and a total amount of melanin were measured. In this study, SK-MEL-3 cells were treated with the media containing 1∼20μM, hydrogen peroxide(H2O2) for 4 hours. H2O2 significantly decreased cell viability compared with control and XTT50 value was determined at 20μM of H2O2. In the antioxidative effect of Rosemary extract, it showed DPPH-radical scavenging activity and SOD-like activity. While, in the melanogenic effect of Rosemary extract, it significantly decreased the tyrosinase activity and a total amount of melanin which were increased by H2O2. From above the results, it is suggested that Rosemary extract was effective in the protection of H2O2-induced cytotoxicity by antioxidative activity and inhibition of melanogenesis.
Abstract
To evaluate the effect of Rosemary(Rosemarinus officinalis) extract on antioxidative activity and melanogenesis on cultured SK-MEL-3, the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, tyrosinase activity and a total amount of melanin were measured. In this study, SK-MEL-3 cells were treated with the media containing 1∼20μM, hydrogen peroxide(H2O2) for 4 hours. H2O2 significantly decreased cell viability compared with control and XTT50 value was determined at 20μM of H2O2. In the antioxidative effect of Rosemary extract, it showed DPPH-radical scavenging activity and SOD-like activity. While, in the melanogenic effect of Rosemary extract, it significantly decreased the tyrosinase activity and a total amount of melanin which were increased by H2O2. From above the results, it is suggested that Rosemary extract was effective in the protection of H2O2-induced cytotoxicity by antioxidative activity and inhibition of melanogenesis.
참고문헌 (24)
- Sankar D, "Effect of Sesame Oil on Diuretics or β-blocker in the modulation of Blood Pessure, Anthropometry, Lipid Profile, and Redox status", Yale J. Biol. Med, 79(1), pp.19~26 (2006)
- Zullyt B, "Antioxidant Mechanism is involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in Rats", Mediators Inflamm, pp.658~673 (2007)
- Kim HM, "Lavender oil inhibits inter mediate-type Allergic reaction in mice and rats", British J. Pharmacol, 51, pp.221~226 (1999)
- Cavanagh HMA, "Biological Activities of Lavender Essential Oil", Phytother Res, 16(4), pp.301~308 (2002)
- Husdon R, "The value of lavender for rest and act ivity in the elderly patient", Compl. Ther. Med, 4, pp.52~57 (1996)
- Itai T, "Psychological effects of aromatherapy on chronic hemodiallsis patients", Psychiatry Clin. Neurosci, 54(4), pp.393~397 (2000)
- Atsumi T, "Smelling lavender and rosemary increases free radical scaveng ing activity and decreases cortisol level in saliva", Psych. Res, 150(1), pp.89~96 (2007)
- Buckle J, "The role of aromatherapy in nursing care", Nurs Clin. Nor. Am, 36(1), pp.57~72 (2001)
- Parviz O, "Antistreptococcal and Antioxidant Activity of Essential Oil from Matricarea chamomile L", Biol. Sci, 2(2), pp.155~160 (2007)
- Lerner AB, "Biochemistry of mela nin formation", Physiol. Rev, 30, pp.92~126 (1984)
- Koiso I, "Development of Recent Melanin Control Agents and Whitening Cosmetics", Fragrance J, 18(6), pp.39~41 (1990)
- Kim YS, "Oligodendroglial cell death in duced by oxygen radicals and its prdu tionby catalase", J. Neurosci. Res, 29, pp.100~106 (1991)
- Jesberger JA, "Oxygen free radicals and brain dysfunction", Int. J. Neurosci, 57, pp.1~17 (1991)
- 한승호, "국내 자생 식물 추출물의 항산화 활성 및 항균효과", 한국약용작물학회지, 14(1), pp.49~55 (2006), 한국약용작물학회
- Chun-Whan Choi, "Antioxidant Constituents and a New Triterpenoid Glycoside from Flos Lonicerae", Archives of Pharmacal Research, 30(1), pp.1~7 (2007), 대한약학회
- Wang L, "Distinctive antioxidant and anti inflammatory effects of flavonols", J. Agric. Food Chem, 54(26), pp.9798~9804 (2006)
- Li YL, "A flavonoid glyco side isolated from Smilax china L. rhi zome in vitro anticancer cell lines", J. Ethnopharmacol, 113(1), pp.115~124 (2007)
- Gates MA, "A prospective study of dietary flavonoid intake and in cidence of epithelial ovarian cancer", Int. J. cancer, 121(10), pp.2225~2232 (2007)
- Michikawa M, "Oxygen radical induced neurotoxicity in spinal cord neuron cultures", J. Neurosci. Res, 37, pp.62~70 (1994)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: Application to pro liferation and cytotoxic assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- Blois MS, "Antioxidant determination by the use of a stable free radica", Nature, 26, pp.1199~1200 (1958)
- Marklund S, "Involvement of super oxide anion radical in the oxidation of pyrogallol and a convenient assay for su peroxide dismutase", Eur. J. Biochem, 47, pp.468~474 (1974)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melano ma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- Choi BW, "Screening of the tyrosinase inhibitors from marine algae and medicinal plants", Kor. J. Pharmacogn, 29, pp.237~242 (1988)