배양 인체피부섬유모세포에서 접촉 피부염 알러젠인 니켈에 대한 차즈기 추출물의 보호 효과
Protective Effect of Perilla silkokiana Nakai Extract on Nickel, Allergen of Contact Dermatitis in Cultured Human Skin Fibroblasts
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To clarify the cytotoxicity and protective effect of Perilla silkokiana Nakai (PSN) extract on nickel, an allergen of contact dermatitis (CD), cell viability was analysed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of nickel sulfate (NiSO4). And also, the effect of antioxidant enzyme, catalase (CAT) on NiSO4-induced cytotoxicity was examined. For the protective effect of PSN extract on NiSO4-induced cytotoxicity, Detroit 551 cells were pretreated with 50 or 70 μg/㎖ of PSN extract for 2 h before the treatment of NiSO4. And also, the antioxidative effects of PSN extract were assessed by DPPHradical scavenging activity and the inhibitory activity of lipid peroxidation. In this study, NiSO4 significantly decreased cell viability dose-dependently compared with control. And the XTT50 value was determined at 110μM of NiSO4. In the effect of antioxidant enzyme, catalase effectively prevented NiSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of PSN extract on NiSO4-induced cytotoxicity,PSN extract significantly increased cell viability which was decreased by NiSO4-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity and the inhibitory activity of lipid peroxidation. From these results, it is suggested that the cytotoxicity of NiSO4 was involved in oxidative stress,and also, PSN extract effectively prevented the cytotoxicity induced by allergen of CD, NiSO4 by antioxidative effect. Conclusively, PSN extract may be a putative resources as an antioxidant for oxidative stress-mediated skin disease via allergen of CD.
Abstract
To clarify the cytotoxicity and protective effect of Perilla silkokiana Nakai (PSN) extract on nickel, an allergen of contact dermatitis (CD), cell viability was analysed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of nickel sulfate (NiSO4). And also, the effect of antioxidant enzyme, catalase (CAT) on NiSO4-induced cytotoxicity was examined. For the protective effect of PSN extract on NiSO4-induced cytotoxicity, Detroit 551 cells were pretreated with 50 or 70 μg/㎖ of PSN extract for 2 h before the treatment of NiSO4. And also, the antioxidative effects of PSN extract were assessed by DPPHradical scavenging activity and the inhibitory activity of lipid peroxidation. In this study, NiSO4 significantly decreased cell viability dose-dependently compared with control. And the XTT50 value was determined at 110μM of NiSO4. In the effect of antioxidant enzyme, catalase effectively prevented NiSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of PSN extract on NiSO4-induced cytotoxicity,PSN extract significantly increased cell viability which was decreased by NiSO4-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity and the inhibitory activity of lipid peroxidation. From these results, it is suggested that the cytotoxicity of NiSO4 was involved in oxidative stress,and also, PSN extract effectively prevented the cytotoxicity induced by allergen of CD, NiSO4 by antioxidative effect. Conclusively, PSN extract may be a putative resources as an antioxidant for oxidative stress-mediated skin disease via allergen of CD.
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