Hydrogen Peroxide로 손상된 배양 인체피부섬유모세포에 대한 함초추출물의 항산화 및 항독효과에 관한 연구
Antioxidative and Detoxic Effects of Salicornia herbacea L. Extract in Cultured Human Skin Fibroblast Damaged by Hydrogen Peroxide
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This study aims to evaluate the antioxidative effect and the detoxic effect of Salicornia herbacea L. (SH) extract. For the examining of antioxidative effect on SH extract, the cell viability of freeze-dried SH (FSH) extract or cold-dried SH (CSH) extract was measured after the cultured human skin fibroblasts (Detroit 551) were pretreated with SH extract for 2 hours before the treatment of hydrogen peroxide (H_2O_2) and also the inhibitory activity of lipid peroxidation was assessed. In the detoxic effect of SH extract, the cell viability was measured by colorimetric assay after Detroit 551 cells were pretreated with FSH extract or CSH extract before the treatment of H_2O_2. In this study, H2O2 significantly decreased cell viability in dose-dependently and the XTT_90 and XTT_50 values were determined at 15 μM and 40 uM of H_2O_2, respectively. In the protective effect of FSH extract against H_2O_2-treated group, FSH extract significantly increased cell viability, compared with H_2O_2-treated group, and also FSH extract showed the inhibitory activity of lipid peroxidation. While, in the detoxic effect of SH extract, FSH extract significantly increase cell viability which were decreased by methylmercuric chloride (MMC)-induced cytotoxicity, but CSH extract did not show any protective effect. From these results, it is suggested that H_2O_2 was highly toxic and SH extract such as FSH extract was effective in the prevention of H_2O_2- or MMC-induced cytotoxicity by antioxidative effect or detoxic effect. Conclusively, FS extract may be a useful agent for the beauty materials by the protective effect on a bleaching agent, H_2O_2 or the heavy metal as the pigment of color make-up product.
Abstract
This study aims to evaluate the antioxidative effect and the detoxic effect of Salicornia herbacea L. (SH) extract. For the examining of antioxidative effect on SH extract, the cell viability of freeze-dried SH (FSH) extract or cold-dried SH (CSH) extract was measured after the cultured human skin fibroblasts (Detroit 551) were pretreated with SH extract for 2 hours before the treatment of hydrogen peroxide (H_2O_2) and also the inhibitory activity of lipid peroxidation was assessed. In the detoxic effect of SH extract, the cell viability was measured by colorimetric assay after Detroit 551 cells were pretreated with FSH extract or CSH extract before the treatment of H_2O_2. In this study, H2O2 significantly decreased cell viability in dose-dependently and the XTT_90 and XTT_50 values were determined at 15 μM and 40 uM of H_2O_2, respectively. In the protective effect of FSH extract against H_2O_2-treated group, FSH extract significantly increased cell viability, compared with H_2O_2-treated group, and also FSH extract showed the inhibitory activity of lipid peroxidation. While, in the detoxic effect of SH extract, FSH extract significantly increase cell viability which were decreased by methylmercuric chloride (MMC)-induced cytotoxicity, but CSH extract did not show any protective effect. From these results, it is suggested that H_2O_2 was highly toxic and SH extract such as FSH extract was effective in the prevention of H_2O_2- or MMC-induced cytotoxicity by antioxidative effect or detoxic effect. Conclusively, FS extract may be a useful agent for the beauty materials by the protective effect on a bleaching agent, H_2O_2 or the heavy metal as the pigment of color make-up product.
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