애플망고 가지 추출물의 항산화 활성 및 대식세포내의 iNOS, COX-2의 염증인자 발현 저해 효과
The Antioxidant Activity of Mangifera indica L. var. irwin Branches Extracts and the Inhibitoty Effect of Inflammatory Factors of iNOS, COX-2 in Macrophages
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In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5–dimethyl–thiazol–2–yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and antiinflammation properties and can be used as a natural ingredient of cosmetic materials.
Abstract
In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5–dimethyl–thiazol–2–yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and antiinflammation properties and can be used as a natural ingredient of cosmetic materials.
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