LPS로 유도한 대식세포에서 레드향 가지 (Citrus reticulata 'Kanpei') 추출물의 항염증 활성
Anti-inflammatory Activity of Citrus reticulata 'Kanpei' Branches Extracts in LPS-induced Macrophages
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초록
Consumers’ interest in natural materials has shown a recent increase; this study was conducted for evaluation of antioxidant activity and anti-inflammatory efficacy in order to determine the potential for application of natural materials using hot water (CBD) and 70% EtOH extract (CBE) of Citrus reticulata 'Kanpei' branches. The results of measurement showed that the polyphenol content of CBD and CBE was 372.97 ± 0.79 mg TAE/100 g and 371.85 ± 0.63 mg TAE/100 g, respectively. The antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity of CBD and CBE was greater than 90% at a concentration of 1,000 μg/mL . The result of evaluation showed that the cell viability of CBD and CBE was greater than 90% at a concentration of 100 μg/mL. The nitric oxide production inhibitory activity of CBD and CBE was 25.41% and 29.26% at a concentration of 100 μg/ mL, respectively, compared with the control group. According to the results from measurement of the level of protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), inhibition of iNOS by CBD and CBE was 7.66% and 28.09% at a concentration of 100 μg/mL, respectively, and 11.69% and 35.43% for COX- 2, respectively. Therefore, the findings of this study verified the antioxidant and anti-inflammatory effects of Citrus reticulata 'Kanpei' branches, confirming their potential for use as natural materials.
Abstract
Consumers’ interest in natural materials has shown a recent increase; this study was conducted for evaluation of antioxidant activity and anti-inflammatory efficacy in order to determine the potential for application of natural materials using hot water (CBD) and 70% EtOH extract (CBE) of Citrus reticulata 'Kanpei' branches. The results of measurement showed that the polyphenol content of CBD and CBE was 372.97 ± 0.79 mg TAE/100 g and 371.85 ± 0.63 mg TAE/100 g, respectively. The antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity of CBD and CBE was greater than 90% at a concentration of 1,000 μg/mL . The result of evaluation showed that the cell viability of CBD and CBE was greater than 90% at a concentration of 100 μg/mL. The nitric oxide production inhibitory activity of CBD and CBE was 25.41% and 29.26% at a concentration of 100 μg/ mL, respectively, compared with the control group. According to the results from measurement of the level of protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), inhibition of iNOS by CBD and CBE was 7.66% and 28.09% at a concentration of 100 μg/mL, respectively, and 11.69% and 35.43% for COX- 2, respectively. Therefore, the findings of this study verified the antioxidant and anti-inflammatory effects of Citrus reticulata 'Kanpei' branches, confirming their potential for use as natural materials.
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