천혜향 잎 추출물의 항산화 활성 및 RAW 264.7 세포에서의 iNOS와 COX-2의 발현 저해 효과
Antioxidant Activities and iNOS, COX-2 Expression Inhibition on RAW 264.7 Cell of ((C. unshiu × C. sinensis) × C. reticulata) × (C. reticulate × C. sinensis) Leaves
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In this study, evaluation of antioxidant and anti-inflammatory activity was performed to confirm the potential for use of Cheonhyehyang leaves as a natural ingredient in cosmetics. Extraction of Cheonhyehyang leaves was performed using hot water extracts (CLD) and 70% ethanol extracts (CLE). The total polyphenol content of CLD and CLE was 210.21 ± 0.19 mg TAE/100 g and 204.14 ± 0.50 mg TAE/100 g, respectively. Electron donating ability was confirmed after measurement, verifying the effectiveness of CLD (79.00%) and CLE (78.91%) at a concentration of 1,000 μg/ mL. The ABTS radical scavenging capacity for CLD and CLE was 99.91% and 99.69% at a concentration of 1,000 μg/mL, respectively. Assessment of cell viability in RAW 264.7 cells performed using an MTT assay showed greater than 90% at a concentration of 100 μg/mL. For assessment of anti-inflammatory activity, production of nitric oxide was inhibited using RAW 264.7 cells induced using lipopolysaccharide; the results showed a decrease according to concentration. Measurement of the inhibitory effects of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was performed using western blot. The result of treatment with CLD and CLE confirmed that expression of iNOS and COX-2 protein was suppressed in a concentration-dependent manner. In conclusion, the findings of this study confirmed the antioxidant and anti-inflammatory effects of CLD and CLE, as well as their potential for use as natural materials.
Abstract
In this study, evaluation of antioxidant and anti-inflammatory activity was performed to confirm the potential for use of Cheonhyehyang leaves as a natural ingredient in cosmetics. Extraction of Cheonhyehyang leaves was performed using hot water extracts (CLD) and 70% ethanol extracts (CLE). The total polyphenol content of CLD and CLE was 210.21 ± 0.19 mg TAE/100 g and 204.14 ± 0.50 mg TAE/100 g, respectively. Electron donating ability was confirmed after measurement, verifying the effectiveness of CLD (79.00%) and CLE (78.91%) at a concentration of 1,000 μg/ mL. The ABTS radical scavenging capacity for CLD and CLE was 99.91% and 99.69% at a concentration of 1,000 μg/mL, respectively. Assessment of cell viability in RAW 264.7 cells performed using an MTT assay showed greater than 90% at a concentration of 100 μg/mL. For assessment of anti-inflammatory activity, production of nitric oxide was inhibited using RAW 264.7 cells induced using lipopolysaccharide; the results showed a decrease according to concentration. Measurement of the inhibitory effects of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was performed using western blot. The result of treatment with CLD and CLE confirmed that expression of iNOS and COX-2 protein was suppressed in a concentration-dependent manner. In conclusion, the findings of this study confirmed the antioxidant and anti-inflammatory effects of CLD and CLE, as well as their potential for use as natural materials.
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