연 부산물 혼합 추출물의 항산화 효능
Antioxidant Activity of Nelumbo nucifera Byproducts Mixed Extract
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In the current study, measurment of the physiological activity and cytotoxites of cosmetic material from pressure heating water extract from Nelumbo nucifera byproducts (shoot, leaves, seedpod) with different extract mixing ratios (ME1, 1:1:1; ME2, 2:1:1; ME3, 1:2:1; ME4, 1:1:2; ME5, 1:2:2:) was performed. The highest contents of total polyphenol and flavonoid compounds were 114.33 mg/g and 259.01 mg/g in ME1. The results of the radical scavenging of ABTS and DPPH, showed that highest scavenging rates, 97.34% and 99.81%, respectively, were detected in ME3 at a concentration of 1,000 ㎍/mL. All extracts exhibited more than 98% ABTS radical scavenging ability at a concentration of 2,000 ㎍/mL, and in measurement of the radical scavenging activity of DPPH, ME1, and ME2, the highest scavenging rates were 93.28% and 93.39%, respectively, at a concentration of 1,000 ㎍/mL. The highest level of superoxide dismutase (SOD)-like activity, 37.65%, was observed for ME1 at a concentration of 2,000 ㎍/mL; the highest inhibition activities, 61.52% and 61.30%, were observed for tyrosinase inhibition in ME2 and ME1. The highest activity, 71.05%, was observed for collagneae inhibition activities in ME1, and the highest astringent effect, 50.64%, was observed in ME3. Cytotoxicity testing of the extract mixture on RAW264.7 macrophages and HaCaT keratinocytes was performed using the MTT assay. Results regarding cell toxicity of RAW264.7 macrophages and HaCaT keratinocytes demonstrated that cell survival was not reduced at a concentration of 25~100 ㎍/mL. Compared with the LPS-treated group, production of NO was reduced by ME1 by approximately 14.55% at a concentration of over 200 ㎍/mL. These results demonstrated the exceptional anti-aging, antioxidant activity of ME1 and ME2. Therefore, it can be concluded that the mixture of shoots, leaves and seedpod of N. nucifera, has potential for use as functional ingredients for improvement of beauty, food, and skin health.
Abstract
In the current study, measurment of the physiological activity and cytotoxites of cosmetic material from pressure heating water extract from Nelumbo nucifera byproducts (shoot, leaves, seedpod) with different extract mixing ratios (ME1, 1:1:1; ME2, 2:1:1; ME3, 1:2:1; ME4, 1:1:2; ME5, 1:2:2:) was performed. The highest contents of total polyphenol and flavonoid compounds were 114.33 mg/g and 259.01 mg/g in ME1. The results of the radical scavenging of ABTS and DPPH, showed that highest scavenging rates, 97.34% and 99.81%, respectively, were detected in ME3 at a concentration of 1,000 ㎍/mL. All extracts exhibited more than 98% ABTS radical scavenging ability at a concentration of 2,000 ㎍/mL, and in measurement of the radical scavenging activity of DPPH, ME1, and ME2, the highest scavenging rates were 93.28% and 93.39%, respectively, at a concentration of 1,000 ㎍/mL. The highest level of superoxide dismutase (SOD)-like activity, 37.65%, was observed for ME1 at a concentration of 2,000 ㎍/mL; the highest inhibition activities, 61.52% and 61.30%, were observed for tyrosinase inhibition in ME2 and ME1. The highest activity, 71.05%, was observed for collagneae inhibition activities in ME1, and the highest astringent effect, 50.64%, was observed in ME3. Cytotoxicity testing of the extract mixture on RAW264.7 macrophages and HaCaT keratinocytes was performed using the MTT assay. Results regarding cell toxicity of RAW264.7 macrophages and HaCaT keratinocytes demonstrated that cell survival was not reduced at a concentration of 25~100 ㎍/mL. Compared with the LPS-treated group, production of NO was reduced by ME1 by approximately 14.55% at a concentration of over 200 ㎍/mL. These results demonstrated the exceptional anti-aging, antioxidant activity of ME1 and ME2. Therefore, it can be concluded that the mixture of shoots, leaves and seedpod of N. nucifera, has potential for use as functional ingredients for improvement of beauty, food, and skin health.
참고문헌 (51)
- AOAC, "Official method of analysis", pp.19~ (2005), Association of official analytical chemists
- Arjun P, "Phytochemical analysis and anticancer activity of Nelumbo nucifera extracts", J Acad Indus, 1, pp.81~85 (2012)
- Arnao MB, "The hydrophilic and lipophilic contribution to total antioxidant activity", Food Chem, 73, pp.239~244 (2001)[DOI]
- Baskaran R, "Neferine from Nelumbo nucifera modulates oxidative stress and cytokines production during hypoxia in human peripheral blood mononuclear cells", Biomed Pharmacother, 93, pp.730~736 (2017)[DOI]
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Boligon AA, "Technical evaluation of antioxidant activity", Med Chem, 4, pp.517~522 (2014)
- Cabanes J, "Kojic acid, a cosmetic skin whitening agent, is a slow binding inhibitor of catecholase activity of tyrosinase", J Pharm Pharmacol, 46, pp.982~985 (1994)[DOI]
- Cha JY, "Effect of potato polyphenolics on lipid peroxidantion in rats", J Korean Soc Food Sci Nutr, 28, pp.1131~1136 (1999)
- Chen Y, "Roburic acid suppresses NO and IL-6 production via targeting NF-κB and MAPK pathway in RAW264. 7 cells", Inflammation, 40, pp.1959~1966 (2017)
- 조영제, "Phellodendron amurense의 미백물질을 이용한 화장품 특성", Journal of Applied Biological Chemistry, 54(2), pp.108~113 (2011), 한국응용생명화학회
- 최소라, "이용부위 및 채취시기에 따른 꾸지뽕나무 메탄올 추출물의 항산화성", 한국약용작물학회지, 17(2), pp.115~120 (2009), 한국약용작물학회
- Chung BS, "Illustration great encyclopedia of herb medicine", pp.2208~ (1990), Younglim Book Centre
- Drmina NS, "Collagenolytic enzymes synthesized by microorganism", Mikrobiologiia, 65, pp.293~304 (1996)
- Dragsted LO, "Antioxidant actions of polyphenols in humans", Int J Vitam Nutr Res, 73, pp.112~119 (2003)[DOI]
- El-Mahmoudy A, "Thymoquinone suppresses expression of inducible nitric oxide synthase in rat macrophages", Int Immunopharmacol, 11, pp.1603~1611 (2002)[DOI]
- Fisher GJ, "Pathophysiology of premature skin aging induced by ultraviolet light", N Engl J Med, 337, pp.1419~1428 (1997)[DOI]
- Vincent J, "Mammalian monophenol monooxygenase(tyrosinase): purification, properties, and reactions catalyzed", Methods Enzymol, 142, pp.154~165 (1987)[DOI]
- Higuchi M, "Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L–arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages", J Immunol, 144, pp.1425~1431 (1990)[DOI]
- Jeon M, "Enzymatic production of rhapontigenin and its application for skin whitening agent", Master, Department of Knowledge-based Technology and Energy, pp.2009
- Jiang XL, "Flavonoid glycosides and alkaloids from the embryos of Nelumbo nucifera seeds and their antioxidant activity", Fitoterapia, 25, pp.184~190 (2018)[DOI]
- Jung SW, "Screening of tyrosinase inhibitor from plants", Korean J Food Sci Technol, 27, pp.891~896 (1995)
- Kang YH, "Studies on the physiological functionally of pine needle and mugwort extracts", Korean J Food Sci Technol, 27, pp.978~984 (1995)
- Kameyama K, "Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1(TRP 1), dopachrome tautormerase(TRP 2)and a melanogenic inhibitor", J Invest Dermatol, 100, pp.126~131 (1993)[DOI]
- Kashiwada Y, "Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids", Bioorg Med Chem, 13, pp.443~448 (2005)[DOI]
- Kim CM, "The encyclopedia of oriental herbal medicine Ⅱ", pp.4623~ (1997), Jeongdam
- Kim IH, "Drug phytology", pp.152~ (1981), Jinmyong Publishing Inc
- Kim JE, "The comparison study of antioxidant and anti-inflammatory effects for raw or fermented lotus (Nelumbo nucifera) leaf extracts", Doctor, Department of Beauty industry, pp.2021
- Kim SH, "Research of traditional herbal medicinals for anti-aging inhibition effect of wrinkle effect in the skin", Kor J Oriental Pathol, 22, pp.691~698 (2008)
- 이진태, "함초의 생리활성기능과 화장품소재로서의 응용", 대한본초학회지, 17(2), pp.6~60 (2002), 대한본초학회
- 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교", 한국약용작물학회지, 12(3), pp.191~202 (2004), 한국약용작물학회
- Lundberg IE, "The role of cytokines, chemokines and adhesion molecules in the pathogenesis of idiopathic, inflammatory myopathies", Current Rheumatology Rep, 2, pp.216~224 (2000)
- Marklund S, "Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~474 (1975)
- Maski H, "Active oxygen scavenging activity of plant extracts", Biol Pharm Bull, 18, pp.162~166 (1995)[DOI]
- Mukherjee PK, "The sacred lotus (Nelumbo nucifera) : phytochemical and therapeutic profile", J Pharm Pharmacol, 61, pp.407~422 (2009)[DOI]
- Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)[DOI]
- 오수정, "함초와 발효 함초 추출물의 화장품 소재로서 생리활성 비교연구", 아시안뷰티화장품학술지, 9(4), pp.1~8 (2011), 한국피부과학연구원
- Ohkuma S, Tatsumi, "Studies on the ventiation system in Nelumbo nucifera Gaertner; growth pattern and gas pressure in lacunae", Rep Tokai Br Cro Sci Japan, 113, pp.23~24 (1992)
- Ono Y, "Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats", J Ethnopharmacol, 106, pp.238~244 (2006)
- Ortonne JP, "Photoprotective porperites of skin melanin", Br J Dermatal, 146, pp.7~10 (2002)
- 박성혜, "혈류개선 효능을 지닌 한약재의 영양성분 분석 및 항산화 활성에 관한 연구", 디지털융복합연구, 14(10), pp.563~570 (2016), 한국디지털정책학회[DOI]
- Pellegrin N, "Screening of dietary carotenoid rich fruit extracts for antioxidant activities applying 2, 2’-azinobis(2-ethylbenzothiazoline-6-sulfonic acid)radical cation de-colorization assay", Method Enzymol, 299, pp.379~389 (1999)[DOI]
- Perron NR, "A review of the antioxidant mechanisms of polyphenol compounds related to iron binding", Cell Biochem Biophys, 53, pp.75~100 (2009)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)[DOI]
- Ryu JH, "Inhibitory activity of plant on nitric oxide synthesis in lipopolysaccharide(LPS)-activated macrophage", Phytother Res, 17, pp.485~489 (2003)[DOI]
- Tsuji N, "implication through selective inhibition of elastase activity", Photochem Photobiol, 74, pp.283~290 (2001)[DOI]
- -bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate reduction by xanthine oxidase, "Anal Biochem", 1997, 206~209, 10.1006[DOI]
- Walsh LJ, "Mast cells and oral inflammation", Critical Reviews in Oral Biology & Med, 14, pp.188~198 (2003)[DOI]
- Wünsch E, "Zur quantitativen bestimmung der collagenase", Hoppe Seylers Z Physiol Chem, 333, pp.149~151 (1963)[DOI]
- Yagi A, "Inhibition of mushroom-tyrosinase by aloe extract", Planta Medica, 53, pp.515~517 (1987)[DOI]
- 양호철, "백련(白蓮)과 홍련(紅蓮)의 부위별 영양성분", 한국식품과학회지, 39(1), pp.14~19 (2007), 한국식품과학회
- 유단희, "연잎 추출물의 항산화 활성 및 멜라노마 세포(B16F10)에서 MITF, TRP-1, TRP-2, tyrosinase의 발현 저해 효과", 생명과학회지, 25(10), pp.1115~1123 (2015), 한국생명과학회