본문 바로가기
KERD
논문KCI 등재

저온숙성 복합한약재 추출물의 항산화능, 항균활성 및 NO 생성 억제능

Antioxidant, Antimicrobial and NO Production Inhibitory Activities of Mixed Medicinal Herb Extracts Aged at Low Temperature in Mineral Water

김미경
대한미용학회 (The Korean Society for Investigative Cosmetology)|2020.12|대한미용학회지 (Journal of Investigative Cosmetology)|제16권 제4호|pp. 343-350|DOI 10.15810/jic.2020.16.4.004

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Antioxidant activityAntimicrobial activityTotal polyphenolLow temperature aged extractNO production inhibitory activity

초록

In order to investigate the possibility of using low-temperature aged mixed medicinal herb extract (Angelica gigas Nakai, Rechmannia glutinosa Libosch, Paeonia lactiflora Pall, Cnidium officinale Makino, Scutellaria baicalensis Georgi) as a material for beauty foods and herbal cosmetics, in the present study, we investigated the yield, total polyphenol content, antioxidant and NO production inhibitory effects of low-temperature aged mixed extracts. The mixed medicinal herbs was precipitated in mineral water and aged at low temperatures. After that, the supernatant was MLE and the precipitate was extracted with water (MLWE) and ethanol (MLEE). As a result, the yield of each extract was much higher in MLE (64.96%) than MLWE (8.30%) and MLEE (7.04%), the total polyphenol content was 283.9 mg TAE/g in MLEE, which was 2.2~3.8 times higher than that in MLE and MLWE. Antioxidation measurements showed that the radical scavenging abilities of DPPH (95.1%) and ABTS (96.7%) were significantly higher at 1,000 μg/mL concentration. In antimicrobial activity test, the MLEE showed a high antimicrobial activity against P. acnes. The cytotoxic concentrations of low temperature aged mixed medicinal herb extracts showed cell viability above 90% at concentrations 100 μg/mL and inhibition of NO production by 52.6% at 100 μg/mL for MLEE. From the results of this study, the ethanol extract of low temperature aged mixed medicinal herb showed a significant effect on the total polyphenol content, antioxidant, and inhibition of NO. Therefore, it is believed that the ethanol extract of low temperature aged mixed medicinal herb can be used as a material for beauty foods and herbal cosmetics.

Abstract

In order to investigate the possibility of using low-temperature aged mixed medicinal herb extract (Angelica gigas Nakai, Rechmannia glutinosa Libosch, Paeonia lactiflora Pall, Cnidium officinale Makino, Scutellaria baicalensis Georgi) as a material for beauty foods and herbal cosmetics, in the present study, we investigated the yield, total polyphenol content, antioxidant and NO production inhibitory effects of low-temperature aged mixed extracts. The mixed medicinal herbs was precipitated in mineral water and aged at low temperatures. After that, the supernatant was MLE and the precipitate was extracted with water (MLWE) and ethanol (MLEE). As a result, the yield of each extract was much higher in MLE (64.96%) than MLWE (8.30%) and MLEE (7.04%), the total polyphenol content was 283.9 mg TAE/g in MLEE, which was 2.2~3.8 times higher than that in MLE and MLWE. Antioxidation measurements showed that the radical scavenging abilities of DPPH (95.1%) and ABTS (96.7%) were significantly higher at 1,000 μg/mL concentration. In antimicrobial activity test, the MLEE showed a high antimicrobial activity against P. acnes. The cytotoxic concentrations of low temperature aged mixed medicinal herb extracts showed cell viability above 90% at concentrations 100 μg/mL and inhibition of NO production by 52.6% at 100 μg/mL for MLEE. From the results of this study, the ethanol extract of low temperature aged mixed medicinal herb showed a significant effect on the total polyphenol content, antioxidant, and inhibition of NO. Therefore, it is believed that the ethanol extract of low temperature aged mixed medicinal herb can be used as a material for beauty foods and herbal cosmetics.

참고문헌 (45)

  1. Ahn SW, "Comparison of biological activities on Rehmannia Radix and R. Radix preparata produced in Korea", Kor J Med Crop Sci, 7, pp.257~262 (1999)
  2. Carmichael JW, "Evaluation of a tetrazolium-based semiautomated colorimetric assay : assessment of chemosensitivity testing", Cancer Res, 47, pp.936~942 (1987)
  3. Cheon GY, "Screening antioxidants of Rehmannia glutinosa", pp.2008
  4. Choi JH, "Studies on the antiaging action of Korean ginseng", J Korean Soc Food Nutr, 17, pp.506~515 (1985)
  5. 최명효, "채취시기 및 가공방법에 따른 섬애약쑥의 이화학적 특성과 항산화활성", Food Science and Preservation, 22(3), pp.399~407 (2015), 한국식품저장유통학회
  6. 최상락, "한약 저온숙성비누가 여드름 피부에 미치는 영향", 대한본초학회지, 34(3), pp.37~44 (2019), 대한본초학회[DOI]
  7. 최상락, "西施玉容散 저온숙성비누의 효능연구", 대한본초학회지, 34(6), pp.125~130 (2019), 대한본초학회[DOI]
  8. 최선영, "머루 과피 용매추출물의 항산화성 및 아질산염 소거작용", 한국식품영양과학회지, 35(8), pp.961~966 (2006), 한국식품영양과학회
  9. Farag RS, "Antimicrobial activity of some Egyptian spice essential oils", J Food Prot, 52, pp.665~670 (1989)
  10. nitrate in biological fluids, "Anal Biochem", 1982, 131~138
  11. 하경희, "저온 숙성에 의한 양념 돈육의 물리적 특성", 한국축산식품학회지, 25(4), pp.397~402 (2005), 한국축산식품학회
  12. Hwang SY, "Influences of cold aged dough on the quality of baguette", Korean J Food & Nutr, 11, pp.369~374 (1998)
  13. 정진아, "식용 및 약용 양치식물에서 부위별 추출물의 항산화활성 탐색", 한국자원식물학회지, 20(2), pp.185~192 (2007), 한국자원식물학회
  14. 정윤숙, "저온숙성마늘 농축액을 첨가한 양갱의 품질특성과 항산화 효과", 한국식품영양학회지, 30(1), pp.1~8 (2017), 한국식품영양학회[DOI]
  15. 전선복, "미강(Rice Bran) 에탄올 추출물의 항산화 활성과 티로시나제 저해 활성", 한국미용학회지, 16(2), pp.602~606 (2010), 한국미용학회
  16. 정동선, "천궁(Cnidium officinale Makino) 지상부(잎과 줄기) 추출물의 항균활성", 한국미생물·생명공학회지, 35(1), pp.30~35 (2007), 한국미생물·생명공학회
  17. 정민화, "5종의 한약재 에탄올 추출물의 항염증 및 표피세포 증식 활성", 생명과학회지, 28(9), pp.1022~1029 (2018), 한국생명과학회[DOI]
  18. Kang HH, "Anti-aging in cosmetics", J Cosmet Sci, 23, pp.57~61 (1997)
  19. 강재란, "전처리 방법을 달리한 섬애약쑥 에탄올 추출물의 이화학적 특성", 생명과학회지, 27(1), pp.23~31 (2017), 한국생명과학회
  20. 박성규, "當歸와 黃？의 배합 변화가 DPPH 자유기 소거에 미치는 영향 연구", 대한본초학회지, 21(1), pp.17~24 (2006), 대한본초학회
  21. Kang YH, "The nitrite scavenging and electron donation ability of phenolic compound", Korean J Food Sci Technol, 28, pp.232~239 (1996)
  22. 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
  23. 김정화, "복분자와 당귀 열수추출물의 마우스를 이용한 항암 및 항스트레스 효과", 한국약용작물학회지, 14(4), pp.206~211 (2006), 한국약용작물학회
  24. 김세종, "작약 잎과 줄기의 생리활성 물질 분리 및 동정", 한국약용작물학회지, 15(1), pp.6~11 (2007), 한국약용작물학회
  25. Kim JY, "Understanding of functional foods for nutritional skin care", J Soc Cosmet Scientists Korea, 30, pp.313~320 (2004)
  26. 김석창, "황금으로부터 항산화 활성 성분의 분리", 한국약용작물학회지, 14(4), pp.212~216 (2006), 한국약용작물학회
  27. Koreck A, "The role of innate immunity in the pathogenesisof acne", Dermatol, 206, pp.96~105 (2003)
  28. Lee EY, "Attitude & perceptions on the skin care health food among working women", pp.2005
  29. 이인철, "한약재 복합 추출물의 항산화, 항균 및 항염 효과", 대한본초학회지, 30(5), pp.51~58 (2015), 대한본초학회
  30. Lee JM, "A research on the awareness and satisfaction of the consumers about cosmetic foods", pp.2012
  31. Lee KS, "The encyclopedia of oriental herbal medicine", pp.4657~4663 (1998), Jungdam Pubishing Co.
  32. Manach C, "Bioavailability and bioefficacy of polyphenols in humans", Am J Clin Nutr, 81, pp.230S~242S (2005)
  33. McCartney-Francis N, "Suppression of arthritis by an inhibitor of nitric oxide synthase", J Exp Med, 178, pp.749~754 (1993)
  34. 모정희, "화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색", 한국인체미용예술학회지, 14(4), pp.49~60 (2013), 한국인체미용예술학회
  35. Nathan C, "Nitric oxide synthases: roles, tolls and controls", Cell, 78, pp.915~918 (1994)
  36. 박현숙, "작약 추출물의 구강병원균에 대한 항균성 및 구강암 세포 증식 억제효과", 한국환경보건학회지, 33(1), pp.21~29 (2007), 한국환경보건학회
  37. 박정진, "추출용매에 따른 울금 추출물의 라디칼 소거능 및 항비만 효과", 한국식품영양과학회지, 42(12), pp.1908~1914 (2013), 한국식품영양과학회
  38. Pellegrini N, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylene- benzothazoline-6-sulfonic acid radical cation decolorization assay", Method Enzymol, 299, pp.379~389 (1999)
  39. Prior RL, "Standardized method for the determination of antioxidant capacity and phenolics in foods and dietary supplement", J Agric Food Chem, 53, pp.4290~4302 (2005)
  40. Shao LX, "Effects of the extract from bergamot and boxthorn on the delay of skin aging and hair growth in mice", Zhongguo Zhong Yao Za Zhi, 28, pp.766~772 (2003)
  41. 신지현, "약용식물 5종의 용매별 추출물 및 조성물의 생리활성", 생명과학회지, 28(3), pp.320~330 (2018), 한국생명과학회[DOI]
  42. Sigleton VL, "Colorimetry of total phenolics with phosphomolybdic : phosphotungstic acid reagents", Am J Enol Viticult, 16, pp.144~158 (1965)
  43. Sim Y, "Studies on compositions and activities of seeential oils in Cnidii Rhizoma", pp.2007
  44. Jin YS, "A study on awareness and usage of inner beauty products in women", pp.2014
  45. Youn NR, "A study on the perception and satisfaction of beauty cosmetic food in middle-aged women", pp.2016

관련 논문AI추천

당귀, 작약, 지황, 천궁 혼합 발효물의 항산화, 항주름 및 미백 효과강희철, 민진우, 엄지나, 주광식한국약용작물학회 · 한국약용작물학회지2017 · 제25권 제3호화장료 소재개발을 위한 5종 약용식물 열수추출 혼합물의 항산화성 연구서수정, 이양숙, 장민정대한미용학회 · 대한미용학회지2021 · 제17권 제3호탄산수로 저온숙성한 황금-사물탕 추출물의 항산화 및 항염증 효과김대용, 김미경대한미용학회 · 대한미용학회지2024 · 제20권 제3호RAW 264.7 cell을 이용한 한방복합추출물의 항염증 효능권경아, 유단희, 이인철대한미용학회 · 대한미용학회지2024 · 제20권 제2호藁本의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구서형식, 황보민대한약침학회 · Journal of Pharmacopuncture2011 · 제14권 제1호황토노각플러스 추출물의 항산화, 항염 및 항미생물 효능이지안, 조준희중소기업융합학회 · 융합정보논문지2020 · 제10권 제12호

이 간행물의 다른 논문 (15건)

UVA 조사된 HaCaT각질형성세포에서 큰금계국 메탄올추출물의 광노화에 대한 보호 효과김영철, 이재순, 오창종pp. 317-322왕벚나무 꽃 생물전환추출물이 섬유아세포의 콜라겐 합성 및 발현에 미치는 효과이승지, 박서연pp. 323-330해양 홍조류 에탄올 추출물의 RAW 264.7 세포와 HEKn 세포에서 항염증 효과김세율, 이숙영, 김일철pp. 331-341마키베리(Aristotelia chilensis)와 포도(Vitis labruscana B.) 혼합 추출물의 모발염색 효과김민관, 서은성pp. 351-356애플망고 가지 추출물의 항산화 활성 및 대식세포내의 iNOS, COX-2의 염증인자 발현 저해 효과유단희, 이인철pp. 357-365
원문 보기