백차 열수추출물의 자외선 조사로 형성된 피부주름 개선 효과
Improving Effects of White Tea Water Extract on Skin Wrinkle Induced by UVB Irradiation in Hairless Mice
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To investigate the improving effects of white tea water extract on skin wrinkle, skin aging animal model was produced by the irradiation of UVB to the backs of hairless mice for 12 weeks. And then experimental materials were applied topically for 4 weeks. Six to seven weeks female SKH-1 hairless mice were divided into five groups including normal (N: no UVB), control (C: UVB + saline), positive control (PC: UVB + 0.01% retinoic acid), experimental 1 (E1: UVB + 1% white tea water extract) and experimental 2 (E2: UVB + 2% white tea water extract) groups, five animals each group. Wrinkles for the C group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the PC, E1 and E2 groups were formed as a pattern of shallow furrows and thin crests. Collagen fibers in dermis of the C group were broken severely and the density was sparse. Whereas collagen fibers in the dermis of the PC, E1 and E2 groups were almost intact with a regular arrangement. Elastosis was found in the dermis of C group with the infiltration of degenerated and clotted elastin. As for the amount of matrix metalloproteinase-3 (MMP-3) expression, PC, E1 and E2 groups were significantly lower than the C group. Therefore, white tea water extract could be used practically for the inhibition or improvement of skin wrinkle induced by UVB irradiation in hairless mice.
Abstract
To investigate the improving effects of white tea water extract on skin wrinkle, skin aging animal model was produced by the irradiation of UVB to the backs of hairless mice for 12 weeks. And then experimental materials were applied topically for 4 weeks. Six to seven weeks female SKH-1 hairless mice were divided into five groups including normal (N: no UVB), control (C: UVB + saline), positive control (PC: UVB + 0.01% retinoic acid), experimental 1 (E1: UVB + 1% white tea water extract) and experimental 2 (E2: UVB + 2% white tea water extract) groups, five animals each group. Wrinkles for the C group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the PC, E1 and E2 groups were formed as a pattern of shallow furrows and thin crests. Collagen fibers in dermis of the C group were broken severely and the density was sparse. Whereas collagen fibers in the dermis of the PC, E1 and E2 groups were almost intact with a regular arrangement. Elastosis was found in the dermis of C group with the infiltration of degenerated and clotted elastin. As for the amount of matrix metalloproteinase-3 (MMP-3) expression, PC, E1 and E2 groups were significantly lower than the C group. Therefore, white tea water extract could be used practically for the inhibition or improvement of skin wrinkle induced by UVB irradiation in hairless mice.
참고문헌 (31)
- 권미화, "백작약 열수추출물의 피부노화 억제 효과( I ) -염증 및 피부장벽 손상 완화 효과를 중심으로", 환경독성보건학회지, 24(2), pp.159~167 (2009), 환경독성보건학회
- 김미지, "한국산 녹차, 우롱차 및 홍차 음료의 cadmium 제 거작용에 관한 연구", 한국영양식량학회지, 23(5), pp.784~791 (1994)
- 석태식, "Hairless 마우스에서 LED 광 조사의 피부주름 개선효과", 대한미용학회지, 6(4), pp.347~356 (2010), 대한미용학회
- 이경옥, "백차 열수추출물의 자외선 조사에 의한 피부염증 및 피부장벽손상 완화 효과", 한국현미경학회지, 41(3), pp.197~204 (2011), 한국현미경학회
- 한국식품의약품안전청, "화장품 기준 및 시험방법(고시 2009-158)" (2009), 한국식품의 약품안전청
- AOAC, "Association of official analytical chemists official methods of analysis (12th)", 127~130 (1995)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Camouse MM, "Topical application of green and white tea extracts provides protection from solar-simulated ultraviolet light in human skin", Exp Dermatol, 18(6), pp.522~526 (2009)
- Chiba K, "Characteristics of skin wrinking and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin", Skin Pharmacol Appl Skin Physiol, 16(4), pp.242~251 (2003)
- Fisher GJ, "Pathophysiology of premature skin aging induced by ultraviolet light", N Engl J Med, 337(20), pp.1419~1428 (1997)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J. Biochem Chem, 12, pp.239~249 (1912)
- Gadow AV, "Comparison of the antioxidant activity of rooibos tea (Aspalathus linearis) with green, Oolong and black teas", Food Chem, 60(1), pp.1977~1997 (1997)
- Gilberto SR, "Potent antimutagenic activity of white tea in comparison with green tea in the Salmonella assay", Gen Tox En Mutagen, 495(1), pp.61~74 (2001)
- Ho CT, "Antioxidative effect of polyphenol extract prepared from various Chinese teas", Prev Med, 21(4), pp.520~525 (1992)
- Honda A, "Interleukin-1 and macrophage migration inhibitory factor (MIF) in dermal fibroblasts mediate UVA-induced matrix metalloproteinase- 1 expression", J Dermatol Sci, 49(1), pp.63~72 (2008)
- Katiyar SK, "Green tea polyphenol (-)- epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress", Carcinog, 22(2), pp.287~294 (2001)
- Kim J, "Protective effects of (-)-epigallocatechin-3-gallate on UVA- and UVBinduced skin damage", Skin Pharmacol Appl Skin Physiol, 14, pp.11~19 (2001)
- Kim MS, "expression after single or repeated exposure to infrared radiation in human skin", Mech Ageing Dev, 127(12), pp.875~882 (2006)
- Kinsella JE, "Possible mechanism for the protective role of antioxidants in wine and plant foods", Food Technol, 47, pp.85~89 (1993)
- 이해준, "자외선 B 조사 마우스에서 피부손상에 대한 홍삼의 효과", Journal of Ginseng Research, 30(4), pp.194~198 (2006), 고려인삼학회
- Manzocco M, "Antioxidant properties of tea extracts as affected by processing", Lebenson Wiss Technol, 31(7), pp.694~698 (1998)
- Moloney SJ, "The hairless mouse model of photoaging: evaluation of the relationship between dermal elastin, collagen, skin thickness and wrinkles", Photochem Photobiol, 56, pp.505~511 (1992)
- Niway Y, "Antioxidant action of natural health products and Chinese herbs", Inflammation, 10(1), pp.79~91 (1986)
- Pasquali-Ronchetti I, "Elastic fiber during development and ging", Microsc Res Tech, 38, pp.428~435 (1997)
- Petersen MJ, "Ultraviolet A irradiation stimulates collagenase production in cultured human fibroblasts", J Invest Dermatol, 99(4), pp.440~444 (1992)
- Rimoldi D, "Chantes in gene expression by 193-and 248-nm excimer laser radiation in cultured human fibroblasts", Rad Res, 131(3), pp.325~331 (1992)
- Shahidi F, "Antioxidants in food and food antioxidants", Nahr, 44(3), pp.158~163 (2000)
- Shirley BW, "Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway", Trends in Plant Sci, 11(1), pp.377~382 (1996)
- Vayalil PK, "Green tea polyphenols prevent ultraviolet light-induced oxidative damage and matrix metalloproteinases expression in mouse skin", J Invest Dermatol, 122(6), pp.1480~1487 (2004)
- Yokozawa T, "In vitro and in vivo studies on the radical- scavenging activity of tea", J Agric Food Chem, 46(4), pp.2143~2150 (1998)
- Zhu QY, "Antioxidative activities of Oolong tea", J Agric Food Chem, 50(23), pp.6929~6934 (2009)