인체피부섬유아세포에서 녹차∙백차∙홍차 열수추출물의 MMP-2 활성 및 MMP-1 유전자 발현 저해능
Inhibitory Efficacy of Water-Soluble Extracts from Green, White and Black Teas on MMP-2 Activity and MMP-1 Gene Expression in Human Dermal Fibroblasts
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This study was conducted to evaluate the inhibitory efficacy of water-soluble extracts from green tea (GT), white tea (WT) and black tea (BT) on MMP-2 activity and MMP-1 gene expression in human dermal fibroblasts. In the experiment of MMP-2 activity using a zymography, at the concentrations of 12.5, 25, and 50μg/mL, EGCG decreased it by 15.4, 24.8(p⁄0.05), and 36.1% (p⁄0.01), GT decreased it by 17.1, 27.2 (p⁄0.01), and 38.7% (p⁄0.01), WT decreased it by 1.7,17.6 (p⁄0.05), and 21.2% (p⁄0.05), and BT decreased it by 37.8 (p⁄0.01), 41.5 (p⁄0.001), and 47.5% (p⁄0.001),respectively, compared to the control group. The order of high to low sequence for the inhibitory efficacy on MMP-2activity was BT¤GT¤EGCG¤WT. In the experiment of MMP-1 gene expression using a RT-PCR, at the concentrations of 12.5, 25, and 50 μg/mL, EGCG significantly (p⁄0.001) decreased it by 71.1, 80.6, and 86.9%, GT significantly (p⁄0.001) decreased it by 35.4, 77.6, and 84.6%, WT significantly (p⁄0.001) decreased it by 32.7, 33.1, and 58.4%, and BT significantly (p⁄0.001) decreased it by 49.2, 81.4, and 88.2%, respectively, compared to the control group. The order of high to low sequence for the inhibitory efficacy on MMP-1 gene expression was BT¤EGCG¤GT¤ WT. These results showed that water-soluble extracts from GT, WT and BT had a high inhibitory efficacy on the collagen degradation in human dermal fibroblasts. Especially black tea, which is fermented tea, showed the highest efficacy among the teas tested in this experiment. even better efficacy than EGCG.
Abstract
This study was conducted to evaluate the inhibitory efficacy of water-soluble extracts from green tea (GT), white tea (WT) and black tea (BT) on MMP-2 activity and MMP-1 gene expression in human dermal fibroblasts. In the experiment of MMP-2 activity using a zymography, at the concentrations of 12.5, 25, and 50μg/mL, EGCG decreased it by 15.4, 24.8(p⁄0.05), and 36.1% (p⁄0.01), GT decreased it by 17.1, 27.2 (p⁄0.01), and 38.7% (p⁄0.01), WT decreased it by 1.7,17.6 (p⁄0.05), and 21.2% (p⁄0.05), and BT decreased it by 37.8 (p⁄0.01), 41.5 (p⁄0.001), and 47.5% (p⁄0.001),respectively, compared to the control group. The order of high to low sequence for the inhibitory efficacy on MMP-2activity was BT¤GT¤EGCG¤WT. In the experiment of MMP-1 gene expression using a RT-PCR, at the concentrations of 12.5, 25, and 50 μg/mL, EGCG significantly (p⁄0.001) decreased it by 71.1, 80.6, and 86.9%, GT significantly (p⁄0.001) decreased it by 35.4, 77.6, and 84.6%, WT significantly (p⁄0.001) decreased it by 32.7, 33.1, and 58.4%, and BT significantly (p⁄0.001) decreased it by 49.2, 81.4, and 88.2%, respectively, compared to the control group. The order of high to low sequence for the inhibitory efficacy on MMP-1 gene expression was BT¤EGCG¤GT¤ WT. These results showed that water-soluble extracts from GT, WT and BT had a high inhibitory efficacy on the collagen degradation in human dermal fibroblasts. Especially black tea, which is fermented tea, showed the highest efficacy among the teas tested in this experiment. even better efficacy than EGCG.
참고문헌 (10)
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