석류잎 추출액의 항산화 작용과 미백작용
Anti-oxidant Activity and Whitening Activity of Punica granatum Leaf Extracts
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To investigate the possibility of development as a whitening agent using pomegranate (Punica granatum L., Punicaceae)leaf extracts, we measured 1-diphenyl-2-picryl hydroazyl (DPPH) assay, nitroblue tetrazolium/xanthine oxidase (NBT/XO)assay, intracellular reactive oxygen species (ROS) scavenging assay, tyrosinase assay and melittin-induced intracellular Ca2+ mobilization and β-hexosaminidase release in B16 melanoma cells. Pomegranate leaf extracts had dose-dependently anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Pomegranate leaf extracts significantly inhibited purified tyrosinase activity using both substrates, L-3,4-dihydroxy-L-phenylalanine (L-DOPA) and L-tyrosine. On the other hand, pomegranate leaf extracts significantly inhibited melittin-induced intracellular Ca2+ mobilization and β-hexosaminidase release in B16 melanoma cells. Therefore, these results suggest that pomegranate leaf extracts may be useful for the development as whitening agents.
Abstract
To investigate the possibility of development as a whitening agent using pomegranate (Punica granatum L., Punicaceae)leaf extracts, we measured 1-diphenyl-2-picryl hydroazyl (DPPH) assay, nitroblue tetrazolium/xanthine oxidase (NBT/XO)assay, intracellular reactive oxygen species (ROS) scavenging assay, tyrosinase assay and melittin-induced intracellular Ca2+ mobilization and β-hexosaminidase release in B16 melanoma cells. Pomegranate leaf extracts had dose-dependently anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Pomegranate leaf extracts significantly inhibited purified tyrosinase activity using both substrates, L-3,4-dihydroxy-L-phenylalanine (L-DOPA) and L-tyrosine. On the other hand, pomegranate leaf extracts significantly inhibited melittin-induced intracellular Ca2+ mobilization and β-hexosaminidase release in B16 melanoma cells. Therefore, these results suggest that pomegranate leaf extracts may be useful for the development as whitening agents.
참고문헌 (19)
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