UVA 조사된 HaCaT각질형성세포에서 큰금계국 메탄올추출물의 광노화에 대한 보호 효과
Protective Effects of Coreopsis lanceolate L. Methanol Extract on UVA-induced Photoaging in HaCaT Keratinocytes
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초록
In order to investigate the protective effects of Coreopsis lanceolate L. methanol extract (CLME) on UVA-induced photoaging in HaCaT human keratinocytes, type I procollagen production, hyaluronic acid (HA) production, and reactive oxygen species (ROS) were investigated in UVA-irradiated HaCaT cells. Treatment with 100 μg/mL CLME increased type I procollagen production by 16.6% (p<0.001), 14.0% (p<0.001), and 26.9% (p<0.001) in 10, 20, and 30 mJ/cm2 UVA-irradiated HaCaT cells, respectively, which were higher than the formation of ascorbic acid (AA) (5.9%, 7.2%, and 17.6%, respectively). Similarly, treatment with 100 μg/mL CLME increased the HA production by 27.3% (p<0.001), which was higher than that of AA (24.9%, p<0.001), in 30 mJ/cm2 UVA-irradiated HaCaT cells. Furthermore, treatment with 100 μg/mL CLME decreased the ROS production by 24.2% (p<0.001), which was higher than that of AA (17.2%, p<0.001), in 30 mJ/cm2 UVA-irradiated HaCaT cells. The results obtained in this study showed that CLME has protective ability on UVA-induced photoaging in HaCaT cells.
Abstract
In order to investigate the protective effects of Coreopsis lanceolate L. methanol extract (CLME) on UVA-induced photoaging in HaCaT human keratinocytes, type I procollagen production, hyaluronic acid (HA) production, and reactive oxygen species (ROS) were investigated in UVA-irradiated HaCaT cells. Treatment with 100 μg/mL CLME increased type I procollagen production by 16.6% (p<0.001), 14.0% (p<0.001), and 26.9% (p<0.001) in 10, 20, and 30 mJ/cm2 UVA-irradiated HaCaT cells, respectively, which were higher than the formation of ascorbic acid (AA) (5.9%, 7.2%, and 17.6%, respectively). Similarly, treatment with 100 μg/mL CLME increased the HA production by 27.3% (p<0.001), which was higher than that of AA (24.9%, p<0.001), in 30 mJ/cm2 UVA-irradiated HaCaT cells. Furthermore, treatment with 100 μg/mL CLME decreased the ROS production by 24.2% (p<0.001), which was higher than that of AA (17.2%, p<0.001), in 30 mJ/cm2 UVA-irradiated HaCaT cells. The results obtained in this study showed that CLME has protective ability on UVA-induced photoaging in HaCaT cells.
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