거봉포도 가지 추출물의 항산화 및 항염증 효능
Antioxidant and Anti-Inflammatory Effects of ‘Kyoho’ Grape (Vitis labruscana L.) Branches Extract
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The antioxidant and anti-inflammatory activity of 70% ethanol extracts of 'Kyoho' grape branches (Vitis labruscana L.) was examined in order to develop a new natural material. First, an evaluation of total polyphenol content and antioxidant activity of 70% ethanol extracts of 'Kyoho' grape branches was performed. The result of measuring the total polyphenol content of 70% ethanol extracts of 'Kyoho' grape branches was 862.41±1.09 mg/100 g. Electron donating ability and 2’-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability were confirmed for evaluation of the antioxidant activity of 70% ethanol extracts of 'Kyoho' grape branches. The results of measurement of antioxidant activity at a concentration of 1,000 µg/mL confirmed that the electron donating ability was 93.09% and the ABTS radical scavenging ability was 99.71%. The MTT assay was performed using RAW 264.7 cells for evaluation of cell viability; the result showed more than 99% at a concentration of 500 µg/mL. Nitric oxide (NO) production inhibitory activity was measured, and Western blot was performed in lipopolysaccharide-induced RAW 264.7 cells for confirmation of the anti-inflammatory activity. The NO production inhibitory activity of 70% ethanol extracts of 'Kyoho' grape branches showed an inhibitory effect of 28.74% at a concentration of 500 µg/mL. Western blot was performed for confirmation of inflammation-related factors iNOS and expression of COX-2 protein. The result confirmed that expression of iNOS and COX-2 protein was inhibited in a concentration-dependent manner. Based on the results described above, the antioxidant and anti-inflammatory activity of 'Kyoho' grape branches, as well as its use as a natural material was confirmed.
Abstract
The antioxidant and anti-inflammatory activity of 70% ethanol extracts of 'Kyoho' grape branches (Vitis labruscana L.) was examined in order to develop a new natural material. First, an evaluation of total polyphenol content and antioxidant activity of 70% ethanol extracts of 'Kyoho' grape branches was performed. The result of measuring the total polyphenol content of 70% ethanol extracts of 'Kyoho' grape branches was 862.41±1.09 mg/100 g. Electron donating ability and 2’-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability were confirmed for evaluation of the antioxidant activity of 70% ethanol extracts of 'Kyoho' grape branches. The results of measurement of antioxidant activity at a concentration of 1,000 µg/mL confirmed that the electron donating ability was 93.09% and the ABTS radical scavenging ability was 99.71%. The MTT assay was performed using RAW 264.7 cells for evaluation of cell viability; the result showed more than 99% at a concentration of 500 µg/mL. Nitric oxide (NO) production inhibitory activity was measured, and Western blot was performed in lipopolysaccharide-induced RAW 264.7 cells for confirmation of the anti-inflammatory activity. The NO production inhibitory activity of 70% ethanol extracts of 'Kyoho' grape branches showed an inhibitory effect of 28.74% at a concentration of 500 µg/mL. Western blot was performed for confirmation of inflammation-related factors iNOS and expression of COX-2 protein. The result confirmed that expression of iNOS and COX-2 protein was inhibited in a concentration-dependent manner. Based on the results described above, the antioxidant and anti-inflammatory activity of 'Kyoho' grape branches, as well as its use as a natural material was confirmed.
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