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복분자 종자 추출물의 항산화 및 항염증 효과

Antioxidant and anti-inflammatory effects of seed ethanol extracts of Rubus coreanus miquel

이성규|강현|황진우
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2022.06|Journal of Plant Biotechnology|제49권 제2호|pp. 155-161|DOI 10.5010/JPB.2022.49.2.155

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키워드

AntioxidantCytotoxicityAnti-inflammatoryEthanol extractsSeed of Rubus coreanus Miq.
주제분류농학

초록

This study investigated the antioxidant and anti-inflammatory effects of seed ethanol extracts from Rubus coreanus Miquel (SERC). To investigate the antioxidant activity, total polyphenol and flavonoid content, ABTS and DPPH radical scavenging activity, and reducing power were measured. The total polyphenol and flavonoid contents in seed ethanol extracts of R. coreanus Miq. were 4.09 μg gallic acid equivalents (GAE)/mg and 16.25 μg quercetin equivalents (QE)/mg, respectively. DPPH and ABTS radical scavenging activity showed concentration-dependent scavenging activity, and the RC50 values of SERC were 26.68 μg/mL and 39.30 μg/mL, respectively. Moreover, the ferric reducing antioxidant power (FRAP) assay was performed to assess the reducing power, and SERC showed 0.61 ± 0.01 mM FeSO4 E/mg. To measure the anti-inflammatory effect, the cytotoxicity and nitric oxide (NO) production inhibitory efficacy in RAW 264.7 cells was confirmed. SERC showed a NO production inhibitory effect at 500 μg/mL without cytotoxicity. As a result of verifying the antioxidant and anti-inflammatory activity using SERC, its potential as an antioxidant and anti-inflammatory material was confirmed.

Abstract

This study investigated the antioxidant and anti-inflammatory effects of seed ethanol extracts from Rubus coreanus Miquel (SERC). To investigate the antioxidant activity, total polyphenol and flavonoid content, ABTS and DPPH radical scavenging activity, and reducing power were measured. The total polyphenol and flavonoid contents in seed ethanol extracts of R. coreanus Miq. were 4.09 μg gallic acid equivalents (GAE)/mg and 16.25 μg quercetin equivalents (QE)/mg, respectively. DPPH and ABTS radical scavenging activity showed concentration-dependent scavenging activity, and the RC50 values of SERC were 26.68 μg/mL and 39.30 μg/mL, respectively. Moreover, the ferric reducing antioxidant power (FRAP) assay was performed to assess the reducing power, and SERC showed 0.61 ± 0.01 mM FeSO4 E/mg. To measure the anti-inflammatory effect, the cytotoxicity and nitric oxide (NO) production inhibitory efficacy in RAW 264.7 cells was confirmed. SERC showed a NO production inhibitory effect at 500 μg/mL without cytotoxicity. As a result of verifying the antioxidant and anti-inflammatory activity using SERC, its potential as an antioxidant and anti-inflammatory material was confirmed.

참고문헌 (39)

  1. Awika JM, "Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products", Journal of Agricultural and Food Chemistry, 51, pp.6657~6666 (2003)
  2. Benzie IF, "The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power the FRAP assay", Analytical Biochemistry, 239, pp.70~76 (1996)
  3. Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  4. Bogdan C, "Nitric oxide synthase in innate and adaptive immunity: an update", Trends in immunology, 36, pp.161~178 (2015)
  5. 차환수, "복분자딸기의 성숙 단계별 생리활성 비교", 한국식품영양과학회지, 36(6), pp.683~688 (2007), 한국식품영양과학회
  6. Cho YJ, "Comparison of physiological activities between hotwater and ethanol extracts of Bokbunja (Rubus coreanum F.)", Journal of the Korean Society of Food Science and Nutrition, 34, pp.790~796 (2005)
  7. 최종화, "그라비올라잎 열수추출물의 항산화 효능 평가", 한국산학기술학회논문지, 18(6), pp.129~135 (2017), 한국산학기술학회[DOI]
  8. 최미란, "노화촉진마우스를 이용한 복분자의 기억력 감퇴 개선 효과", 한국식품영양과학회지, 41(9), pp.1253~1259 (2012), 한국식품영양과학회
  9. Duh PD, "Action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage", Food and Chemical Toxicology, 37(11), pp.1055~1061 (1999)
  10. Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, 12(2), pp.239~243 (1912)
  11. Hertog MGL, "Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juices", Journal of Agricultural and Food Chemistry, 41, pp.1242~1246 (1993)
  12. Hertog MGL, "Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits", Journal of Agricultural and Food Chemistry, 40, pp.1591~1159 (1992)
  13. 황진우, "Antioxidant and Anti-inflammatory Effects of Hot Water and Ethanol Extracts from Endemic Plants in Indonesia", 대한의생명과학회지, 27(3), pp.161~169 (2021), 대한의생명과학회
  14. 황보현, "산수유의 채취 부위에 따른 기능 성분 분석과 항산화 및 항염증 효과에 관한 연구", 생명과학회지, 31(3), pp.287~297 (2021), 한국생명과학회[DOI]
  15. 장민정, "화장료 소재로서 뜰보리수 과실 추출물의 항산화, 항염증 및 항노화 활성", 대한미용학회지, 17(2), pp.159~167 (2021), 대한미용학회[DOI]
  16. 전혜린, "고지방식이 섭취 마우스를 이용한 복분자종자유의 혈행 개선 효과", 한국식품영양과학회지, 44(8), pp.1105~1113 (2015), 한국식품영양과학회
  17. 강현, "복분자 에탄올 추출물 및 분획물의 항산화 효과", Journal of Plant Biotechnology, 48(4), pp.264~270 (2021), 한국식물생명공학회[DOI]
  18. 이현용, "저온 추출 공정에 의한 마황과 복분자의 면역 활성 증진 효과", 한국약용작물학회지, 13(3), pp.81~86 (2005), 한국약용작물학회
  19. 김현모, "염생식물 도깨비고비의 항산화 및 항염증 효과", Ocean and Polar Research, 43(3), pp.113~126 (2021), 한국해양과학기술원[DOI]
  20. 김인덕, "한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과", KSBB Journal, 23(6), pp.529~534 (2008), 한국생물공학회
  21. 김정화, "복분자와 당귀 열수추출물의 마우스를 이용한 항암 및 항스트레스 효과", 한국약용작물학회지, 14(4), pp.206~211 (2006), 한국약용작물학회
  22. 김준혁, "14종 국화과(Asteraceae) 식물 종자 추출물의 항산화 활성", 생명과학회지, 31(6), pp.543~549 (2021), 한국생명과학회[DOI]
  23. 김지명, "숙성에 따른 토종 복분자 딸기의 특성", 한국식품과학회지, 43(3), pp.341~347 (2011), 한국식품과학회
  24. 김진영, "Change of Phenylpropanoic Acid and Flavonol Contents at Different Growth Stage of Glasswort (Salicornia herbacea L.)", Food Science and Biotechnology, 23(3), pp.685~691 (2014), 한국식품과학회[DOI]
  25. 김성조, "한약탕제찌꺼기 발효퇴비 처리에 따른 복분자과실의 생리활성", 한국자원식물학회지, 24(2), pp.243~252 (2011), 한국자원식물학회
  26. 구강모, "옥수수 수염의 항산화 활성과 기능성분 분석", 한국자원식물학회지, 22(4), pp.323~329 (2009), 한국자원식물학회
  27. 권유리, "추출용매에 따른 모링가(Moringa oleifera Lam.) 잎의 항산화 및 생리활성 효과", 한국식품저장유통학회지, 21(6), pp.831~837 (2014), 한국식품저장유통학회
  28. Lee GW, "Antioxidant Activity of Leaf Extract from Annona muricata", Review of Korea Contents Association, 14(3), pp.43~46 (2016)
  29. 이정은, "갓(Brassica juncea L. Czern.)의 잎과 종자 추출물의 항산화, 미백 및 항주름 효능", 아시안뷰티화장품학술지, 18(3), pp.283~295 (2020), 한국피부과학연구원[DOI]
  30. Lee JW, "Determination of total phenolic compounds from the fruit of Rubus coreanum and antioxidative activity", Journal of the Korean Society of Food Science and Nutrition, 29, pp.943~947 (2000)
  31. 이민정, "고콜레스테롤 식이 투여 마우스에서 복분자 미숙과 추출물과 홍삼농축액 복합 투여의 콜레스테롤 개선 효과", 한국식품영양과학회지, 43(10), pp.1491~1499 (2014), 한국식품영양과학회
  32. 이승은, "항염증 및 항산화 활성 보유 유용 식물 탐색", 한국자원식물학회지, 26(4), pp.441~449 (2013), 한국자원식물학회[DOI]
  33. 이수정, "복분자 미숙과와 홍삼 추출물의 콜레스테롤 개선 효과", 한국식품과학회지, 45(5), pp.628~635 (2013), 한국식품과학회[DOI]
  34. Moncada, S, "The L-arginine-nitric oxide pathway", The New England Journal of Medicine, 329, pp.2002~2012 (1993)
  35. Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", Journal of Ethnopharmacology, 71(1-2), pp.109~114 (2000)
  36. Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology & Medicine, 26(9-10), pp.1231~1237 (1999)
  37. 김진경, "토종 복분자와 외래종 복분자 추출물의 항염증효과 비교", 한국식품과학회지, 39(3), pp.342~347 (2007), 한국식품과학회
  38. 윤인, "복분자 열매에서 항산화활성을 지닌 quercetin의 분리 및 동정", 한국식품과학회지, 35(3), pp.499~502 (2003), 한국식품과학회
  39. 유지송, "대장균과 황색포도상구균에 대한 복분자 추출물의 항균효과분석", 한국식품저장유통학회지, 26(3), pp.360~364 (2019), 한국식품저장유통학회

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