건조방법에 따른 포도 송이가지의 항산화 효과 및 생리활성 특성
Comparison of the antioxidant effects and physiological activities of grape fruit stem extracts derived with different drying methods
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This study was conducted to examine the utilization potential of grape stems as nutritional supplements, and the physiological functionalities of 70% ethanol extracts from grape fruit stem (GFS) were investigated. Each experimental group was prepared with different methods and included GFSF (GFS prepared with freeze drying), GFSI (GFS prepared with infrared drying), GFSH (GFS prepared with heat air drying), and GFSS (GFS prepared with sun drying). The respective yields of freeze-dried powders for the GFSF, GFSI, GFSH, and GFSS were 59.27%, 57.13%, 58.57%, and 58.87%, respectively. Total polyphenol contents in the GFSF were significantly greater than those in the other extracts, whereas total flavonoid contents in the GFSI were higher than those in the other extracts. The contents of proanthocyanidin-related substances were ranked in the order of GFSF > GFSI > GFSH > GFSS. The thin layer chromatograph (TLC) analysis of catechin showed that the GFSF, GFSI, GFSH, and GFSS were detected in the same band. The electron donating ability with 500 µg/mL (w/v) solutions of GFSF, GFSI, GFSH, and GFSS amounted to 93.14%, 93.07%, 92.64%, and 86.95%, respectively, and the reducing powers (OD 700) were 1.933, 1.765, 1.455, and 1.200 absorbance units, respectively. Additionally, the ABTS radical scavenging ability showed the same tendency that was observed with the electron donating ability and reducing power. The angiotensin-converting enzyme (ACE) inhibitory activity and tyrosinase inhibitory activity with 500 µg/mL (w/v) solutions of GFSF and GFSI were higher than those of GFSH and GFSS. In conclusion, the infrared drying technique is the superior method for the enhancement of biological activity for by-product utilization.
Abstract
This study was conducted to examine the utilization potential of grape stems as nutritional supplements, and the physiological functionalities of 70% ethanol extracts from grape fruit stem (GFS) were investigated. Each experimental group was prepared with different methods and included GFSF (GFS prepared with freeze drying), GFSI (GFS prepared with infrared drying), GFSH (GFS prepared with heat air drying), and GFSS (GFS prepared with sun drying). The respective yields of freeze-dried powders for the GFSF, GFSI, GFSH, and GFSS were 59.27%, 57.13%, 58.57%, and 58.87%, respectively. Total polyphenol contents in the GFSF were significantly greater than those in the other extracts, whereas total flavonoid contents in the GFSI were higher than those in the other extracts. The contents of proanthocyanidin-related substances were ranked in the order of GFSF > GFSI > GFSH > GFSS. The thin layer chromatograph (TLC) analysis of catechin showed that the GFSF, GFSI, GFSH, and GFSS were detected in the same band. The electron donating ability with 500 µg/mL (w/v) solutions of GFSF, GFSI, GFSH, and GFSS amounted to 93.14%, 93.07%, 92.64%, and 86.95%, respectively, and the reducing powers (OD 700) were 1.933, 1.765, 1.455, and 1.200 absorbance units, respectively. Additionally, the ABTS radical scavenging ability showed the same tendency that was observed with the electron donating ability and reducing power. The angiotensin-converting enzyme (ACE) inhibitory activity and tyrosinase inhibitory activity with 500 µg/mL (w/v) solutions of GFSF and GFSI were higher than those of GFSH and GFSS. In conclusion, the infrared drying technique is the superior method for the enhancement of biological activity for by-product utilization.
참고문헌 (32)
- Halliwell B, "Reactive oxygen species and the central nervous system", J Neurochem, 59, pp.1609~1623 (2006)
- 황인택, "잡초 및 농림부산물을 이용한 Biorefinery 기술개발", Weed&Turfgrass Science, 30(4), pp.340~360 (2010), 한국잡초학회
- 최상원, "용매추출 및 화학적 정제법에 의한 고품질의 포도씨유의 제조", Food Science and Preservation, 12(6), pp.600~607 (2005), 한국식품저장유통학회
- Mi-Ae Yoo, "Evaluation of Physicochemical Properties in Different Cultivar GrapeSeed Waste", Food Science and Biotechnology, 13(1), pp.26~29 (2004), 한국식품과학회
- Joe AK, "Resveratrol induces growth inhibition. S-phase arrest, apophysis and changes in biomarker expression in several human cancer cell line", Clin Cancer Res, 8, pp.893~903 (2002)
- Gao X, "Disparate in vitro and in vivo antileukemic effects of resveratrol, a natural polyphenolic compound found in grapes", J Nutr, 132, pp.2076~2081 (2002)[DOI]
- 조용진, "국내산 포도의 부위별 레스베라트를 함량", 한국식품과학회지, 35(2), pp.306~308 (2003), 한국식품과학회
- 조지은, "건조방법과 감마선 조사에 따른 포도가공부산물의 색상 변화 및 항산화활성", 한국식품영양과학회지, 39(12), pp.1826~1831 (2010), 한국식품영양과학회
- 육홍선, "건조방법에 따른 포도 가공부산물의 품질특성", 한국식품영양과학회지, 39(9), pp.1353~1358 (2010), 한국식품영양과학회
- Dewanto V, "Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity", J Agric Food Chem, 50, pp.3010~3014 (2002)[DOI]
- Saleh ES, "Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples", Food Chem, 114, pp.1271~1277 (2008)
- Sun B, "Critical factors of vanillin assay for catechins and proanthocyanidins", J Agric Food Chem, 46, pp.4267~4274 (1998)[DOI]
- Andriambeloson E, "Natural dietary polyphenolic compounds cause endothelium-dependent vasorelaxation in rat thoracic aorta", J Nutr, 128, pp.2324~2333 (1998)[DOI]
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)[DOI]
- Arabshahi-Delouee S, "Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves", Food Chem, 102, pp.1233~1240 (2007)[DOI]
- Re R, "Antioxidant activity applying an improved ABTS radial cation decolorization assay", Free Radical Biol Med, 26, pp.1231~1237 (1999)[DOI]
- Cushman DW, "Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung", Biochem Pharmacol, 20, pp.1637~1648 (1971)[DOI]
- 정다솜, "건조방법에 따른 포도씨의 항산화 활성의 변화", Food Science and Preservation, 23(1), pp.1~6 (2016), 한국식품저장유통학회
- 차재영, "함초(Salicornia herbacea L.)의 채취 시기별 이화학적 특성 탐색", 생명과학회지, 16(4), pp.683~690 (2006), 한국생명과학회
- da Silva JMR, "Procyanidin dimers and trimers from grape seeds", Phytochemistry, 30, pp.1259~1264 (1991)[DOI]
- 김교연, "원료삼의 증삼 및 건조 조건별 홍삼의 품질 및 기능성", 한국식품과학회지, 39(5), pp.494~499 (2007), 한국식품과학회
- Kim JH, "Comparison of the chemial compositions of Korean and Chinese safflower (Carthamus tinctororius L.) seed", Korean J Food Sci Technol, 31, pp.912~918 (1999)
- 강호경, "저장 전 열처리가 저온 저장 중 '신고'배의 과피 흑변과 페놀성 화합물의 함량 변화에 미치는 영향", Horticulture, Environment, and Biotechnology, 44(2), pp.197~200 (2003), 한국원예학회
- Xu C, "Phenolic compounds and antioxidant properties of different grape cultivars grown in China", Food Chem, 119, pp.1557~1565 (2010)[DOI]
- Cosme F, "Effect of various proteins on different molecular weight proanthocyanidin fractions of red wine during wine fining", Am J Enol Vitic, 60, pp.74~81 (2009)
- 정햇님, "전처리 및 건조방법에 따른 가시오갈피생약재의 품질변화", 한국약용작물학회지, 18(2), pp.98~104 (2010), 한국약용작물학회
- 김연주, "건조방법을 달리한 감말랭이의 이화학적 및 관능적 품질 특성", 한국식품과학회지, 41(1), pp.64~68 (2009), 한국식품과학회
- 남산, "건조 방법에 따른 산사과 추출물의 항산화 및 생리활성", Food Science and Preservation, 23(2), pp.246~251 (2016), 한국식품저장유통학회
- Osawa T, "Postharvest Biochemistry of Plant Food Material in the Tropics", pp.241~251 (1994), Japan Scientific Societies Press
- Luximon-Ramma A, "Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula", J Agric Food Chem, 50, pp.5042~5047 (2002)[DOI]
- Kim KM, "Chemical structure of angiotensin converting enzyme inhibitor isolated from onion flesh", Food Sci Biotechnol, 8, pp.329~332 (1999)
- Kameda K, "Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity", J Nat Prod, 50, pp.680~683 (1987)[DOI]