농도별 올레인산이 골격근 근원세포 증식과 분화에 미치는 영향
Effects of Oleic Acid of Different Concentrations on Myoblast Proliferation and Myogenic Differentiation
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본 연구는 올레인산(Oleic acid)이 근육위성세포에 미치는 영향을 분석하기 위해 근원세포(Myoblast)의 활동 을 in vitro수준에서 확인하였다. 구체적으로 올레인산이 근원세포의 증식과 근관세포(Myotube)로의 분화에 미치는 영향을 확인하고 이와 관련한 주요 신호인자를 연구하였다. 근원세포(C2C12)를 배양 후 올레산을 처 치한 결과, 농도별(0, 100, 300, 500μM) 근원세포 생존률에서는 통계적인 차이를 확인하지 못하였으나, 근원 세포의 수, 근관세포의 융합지수, 사이즈, PGC-1α의 mRNA발현량은 올레인산의 농도 의존적으로 유의미 하게 증가됨을 확인하였다. 이 결과는 올레인산이 근원세포의 증식과 분화를 촉진하고 분화된 근관세포의 미토콘드리아 생합성을 증가시킴을 보여준다. 결론적으로 본 연구는 골격근 회복 및 재생과 연관하여 올레 인산의 잠재적 임상적 가능성을 세포학적으로 입증한 것이라 할 수 있다.
Abstract
The purpose of this study was to investigate the effects of oleic acid on myoblast proliferation, myogenic differentiation and mRNA expression of PGC-1α in differentiated myotubes. The level of myoblasts viability was not different in all myoblasts treated with oleic acid of different concentrations (0, 100, 300, 500μM). Myoblsts number, myotube fusion index and width, mRNA expression of PGC-1α were increased in a dose dependent manner, confirming that oleic acid promotes myoblasts activities possibility via mitochondrial biogenesis. These results provide a piece of cellular evidence that oleic acid could be considered as a potential therapeutic agent to promote satellite cell-mediated skeletal muscle regeneration following muscle injury.
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