PGC-1α 단백질이 생쥐 근육세포의 글리코겐 분해 및 해당효소의 발현을 조절한다
The Expression of PGC-1α Protein Regulates Glycogenolytic and Glycolytic Enzyme in Mouse Skeletal Muscle Cell
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본 연구는 골격근 세포인 C2C12 myotube에 ppargc1a(PGC-1α 유전자) mRNA를 silence시키는 기법으로 PGC-1α의 발현을 부분적으로 억제하여 글리코겐 분해 및 해당 효소의 발현에 어떠한 영향을 미치는지 확인하는 것이다. C2C12 골격근 세포에 PGC-1α의 과발현을 유도하기 위하여 Ad-PGC-1α adenovirus를 처치하였으며, PGC-1α의 발현을 억제하기 위하여 ppargc1a mRNA를 silence 시키는 기법을 이용하였다. 골격근 세포에 PGC-1α의 과발현을 유도한 결과, PFK의 발현이 empty vector를 처치한 그룹보다 0.4배 감소한 것으로 나타났다. 골격근 세포에 PGC-1α의 발현을 부분적으로 억제시킨 결과 phosphorylase kinase, glycogen phosphorylase 및 PFK는 empty vector를 처치한 그룹보다 각각 2배, 1.4배, 1.5배 증가한 것으로 나타났다. 본 연구의 결과는 단기간 지구성 운동 후 빠르게 증가하는 PGC-1α가 골격근 세포 내 글리코겐 분해효소 및 해당효소를 간접적으로 조절하여 글리코겐 절약효과를 유도할 수 있음을 증명하였다.
Abstract
The purpose of this study was to evaluate the role of PGC-1α on the expression of glycolytic and glycogenolytic enzyme in C2C12 mouse skeletal muscle cell. PGC-1α overexpression in C2C12 myotube was achieved by PGC-1α encoded adenovirus. When PGC-1α was overexpressed, PFK expression was suppressed 0.4 fold compared to control, empty vector treated cell. Contrary to PGC-1α overexpression, silencing ppargc1a mRNA (suppressed PGC-1α protein expression) induced 2, 1.4 and 1.5 fold increase in phosphorylase kinase, glycogen phosphorylase, and PFK protein expression respectively. In summary, down regulation of expression of the glycogenolytic and glycolytic enzymes in muscle that mediates a slowing of muscle glycogen depletion appears to be through the PGC-1α expression.
참고문헌 (21)
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