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추출방법에 따른 하니베리의 항산화 활성 비교 평가 연구

Comparative Evaluation of Extraction and Processing Methods on Antioxidative Contents and Radical Scavenging Activity of Honeyberry

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초록

Honeyberry (Lonicera caerulea) is rich in phenolic acids and flavonoids which is beneficial effect on health. The present study was aimed to investigate antioxidative effectiveness of honeyberry extracts with different solvents and temperature. The samples of honeyberry extract were prepared in four different extraction conditions, which were 25℃ water (HW25), 60℃ water (HW60), 25% ethanol (HE25), and 70% ethanol (HE70). ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), radical scavenging activity, reducing power and TPC (total polyphenol contents), TFC (total flavonoid contents) and TAC (total anthocyanin contents) were significantly higher in ethanol extracts than in water extracts of honeyberry. Unlike another results, DPPH (2,2-Diphenyl-1-picrylhydrazyl) was significantly higher in water extracts than in ethanol extracts of honeyberry. All of TPC (179.63 mg GAE/g), TFC (213.67 RE/g) and TAC (94.01 mg/L) were the highest in HE25, and both ABTS radical scavenging activity (54.99%) and reducing power (0.41) were the highest in HE25. However DPPH (2,2-Diphenyl-1-picrylhydrazyl) was the highest in HW60. In conclusion, 25% ethanol extracts of honeyberry increased in radical scavenging activities (Reducing power and ABTS), TPC, TFC and TAC. Our results suggest that honeyberry 25% ethanol extracts might be exploited for functional foods and nutraceutical applications.

Abstract

Honeyberry (Lonicera caerulea) is rich in phenolic acids and flavonoids which is beneficial effect on health. The present study was aimed to investigate antioxidative effectiveness of honeyberry extracts with different solvents and temperature. The samples of honeyberry extract were prepared in four different extraction conditions, which were 25℃ water (HW25), 60℃ water (HW60), 25% ethanol (HE25), and 70% ethanol (HE70). ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), radical scavenging activity, reducing power and TPC (total polyphenol contents), TFC (total flavonoid contents) and TAC (total anthocyanin contents) were significantly higher in ethanol extracts than in water extracts of honeyberry. Unlike another results, DPPH (2,2-Diphenyl-1-picrylhydrazyl) was significantly higher in water extracts than in ethanol extracts of honeyberry. All of TPC (179.63 mg GAE/g), TFC (213.67 RE/g) and TAC (94.01 mg/L) were the highest in HE25, and both ABTS radical scavenging activity (54.99%) and reducing power (0.41) were the highest in HE25. However DPPH (2,2-Diphenyl-1-picrylhydrazyl) was the highest in HW60. In conclusion, 25% ethanol extracts of honeyberry increased in radical scavenging activities (Reducing power and ABTS), TPC, TFC and TAC. Our results suggest that honeyberry 25% ethanol extracts might be exploited for functional foods and nutraceutical applications.

참고문헌 (57)

  1. 고윤정, "항산화 활성 증진을 위한 민들레 잎의 열수추출 조건의 최적화", 한국식품과학회지, 40(3), pp.283~289 (2008), 한국식품과학회
  2. 김명기, "노니 부정근으로부터 유효성분의 최적 추출조건", 한국식품과학회지, 48(2), pp.111~116 (2016), 한국식품과학회[DOI]
  3. 박혜진, "추출 온도와 시간이 옻나무 추출물의 항산화활성에 미치는 영향", 한국식품영양과학회지, 42(11), pp.1776~1782 (2013), 한국식품영양과학회
  4. 손종연, "밀, 쌀, 현미 및 보리로 제조한 시판 국수류의 항산화 특성", 한국외식산업학회지, 10(2), pp.95~103 (2014), 한국외식산업학회
  5. 이지영, "댕댕이 나무 추출물을 유효성분으로 함유하는 화장료 조성물", 특허출원번호 10-2014-0078865, ~ (2014)
  6. 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
  7. 정세현, "약재 추출물을 첨가한 노인용 기능성 양갱의 품질 특성 및 항산화 효과", 한국식품영양과학회지, 44(9), pp.1304~1310 (2015), 한국식품영양과학회
  8. 정중근, "한국산 고구마의 기관별, 부위별, 품종별, 조리방법에 따른 총 폴리페놀 함량과 항산화 활성에 관한 연구", 한국외식산업학회지, 9(2), pp.7~19 (2013), 한국외식산업학회
  9. 조애경, "장미, 동백 및 맨드라미꽃의 항산화 활성", 한국외식산업학회지, 11(1), pp.21~33 (2015), 한국외식산업학회
  10. 조성훈, "댕댕이 나무 열매 추출물을 유효성분으로 함유하는 허혈성 뇌혈관질환의 예방 또는 개선용 약학적 조성물 또는 건강식품", 특허출원번호 WO2013141581 A1, ~ (2013)
  11. Blois, M.S., "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  12. Borriello, A., "Dietary polyphenols: focus on resveratrol, a promising agent in the prevention of cardiovascular diseases and control of glucose homeostasis", Nutrition, Metabolism & Cardiovascular Diseases, 20(8), pp.618~625 (2010)
  13. Bouayed, J., "Exogenous antioxidantsdouble-edged swords in cellular redox state: health beneficial effects at physiologic doses versus deleterious effects at high doses", Oxidative medicine and cellular longevity, 3(4), pp.228~237 (2010)
  14. Bridle, P., "Anthocyanins as natural food colours—selected aspects", Food Chemistry, 58(1), pp.103~109 (1997)
  15. Caprioli, G., "Blue honeysuckle fruit (Lonicera caerulea L.) from eastern Russia: phenolic composition, nutritional value and biological activities of its polar extracts", Food Function, 7(4), pp.1892~1903 (2016)
  16. 조성빈, "오미자 색소 추출물의 가열 변색에 대한 속도론적 연구", 한국식품과학회지, 35(1), pp.23~27 (2003), 한국식품과학회
  17. Chou, S.T., "Antioxidative activity and safety of 50% ethanolic red bean extract (Phaseolus radiatus L. var. Aurea)", Journal of food science, 68(1), pp.21~25 (2003)
  18. Davis, W., "Determination of flavanones in citrus fruits", Analytical Chemistry, 19(7), pp.476~478 (1947)
  19. Decker, E.A., "Differences in the antioxidant mechanism of carnosine in the presence of copper and iron", Journal of Agricultural and Food Chemistry, 40(5), pp.756~759 (1992)
  20. Duh, P.D., "Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free-radical and active oxygen", Journal of the American Oil Chemists' Society, 75(4), pp.455~461 (1998)
  21. Fomenko, S.E., "Hepatorotective activity of honeysuckle fruit extract in carbon tetrachloride intoxicated rats", Eksperimental'naia i klinicheskaia farmakologiia, 77(10), pp.26~30 (2014)
  22. Francis, F.J., "Food colorants: anthocyanins", Critical Reviews in Food Science & Nutrition, 28(4), pp.273~314 (1989)
  23. Huang, D., "The chemistry behind antioxidant capacity assays", Journal of agricultural and food chemistry, 53(6), pp.1841~1856 (2005)
  24. 황초롱, "열처리 온도에 따른 더덕과 도라지의 화학성분과 항산화활성", 한국식품영양과학회지, 40(6), pp.798~803 (2011), 한국식품영양과학회
  25. Ito, N., "Studies on antioxidants: their carcinogenic and modifying effects on chemical carcinogenesis", Food and Chemical Toxicology, 24(10-11), pp.1071~1082 (1986)
  26. Jang, E., "Antioxidant effect of omija (Schizandra chinesis Baillon) extracts", Journal of the Korean Society of Food Science and Nutrition, 12(5), pp.372~376 (1996)
  27. Jin, X. H., "Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo", Experimental Eye Research, 82(5), pp.860~867 (2006)
  28. Jurgonski, A., "An anthocyanin-rich extract from Kamchatka honeysuckle increases enzymatic activity within the gut and ameliorates abnormal lipid and glucose metabolism in rats", Nutritions, 29(6), pp.898~902 (2013)
  29. Jurikova, T., "Phenolic profile of edible honeysuckle berries (genus lonicera) and their biological effects", Molecules, 17(1), pp.61~79 (2011)
  30. Kawashima, S., "The possible role of lipoperoxide in aging", Nagoya journal of medical science, 32(2), pp.303~326 (1970)
  31. Kumaran, A., "In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India", Food Science and Technology, 40(2), pp.344~352 (2007)
  32. Lee, J., "Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study", Journal of AOAC International, 88(5), pp.1269~1278 (2005)
  33. Leng, P., "Anthocyanin accumulation in apple and peach shoots during cold acclimation", Scientia Horticulturae, 83(1), pp.43~50 (2000)
  34. Małodobry, M., "Evaluation of the yield and some components in the fruit of blue honeysuckle (Lonicera caerulea var. edulis Turcz. Freyn.)", Folia Horticulturae, 22(1), pp.45~50 (2010)
  35. Meir, S., "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves", Journal of agricultural and food chemistry, 43(7), pp.1813~1819 (1995)
  36. Oszmiański, J., "Effect of dried powder preparation process on polyphenolic content and antioxidant activity of blue honeysuckle berries (Lonicera caerulea L. var. kamtschatica)", Food Science and Technology, 67, pp.214~222 (2016)
  37. Palikova, I., "Constituents and antimicrobial properties of blue honeysuckle: a novel source for phenolic antioxidants", Journal of agricultural and food chemistry, 56(24), pp.11883~11889 (2008)
  38. Palikova, I., "Protectivity of blue honeysuckle extract against oxidative human endothelial cells and rat hepatocyte damage", Journal of agricultural and food chemistry, 57(15), pp.6584~6589 (2009)
  39. Raudsepp, P., "The antioxidative and antimicrobial properties of the blue honeysuckle (Lonicera caerulea L.), Siberian rhubarb (Rheum rhaponticum L.) and some other plants, compared to ascorbic acid and sodium nitrite", Food Control, 31(1), pp.129~135 (2013)
  40. Re, R., "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology & Medicine Med, 26(9-10), pp.1231~1237 (1999)
  41. Sánchez-Moreno, C., "Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems", Food science and technology, 8(3), pp.121~137 (2002)
  42. Santangelo, C., "Polyphenols, intracellular signalling and inflammation", Annali dell'Istituto Superiore di Sanità, 43(4), pp.394~405 (2007)
  43. Schieber, M., "ROS function in redox signaling and oxidative stress", Current Biology, 24(10), pp.453~462 (2014)
  44. Shi, J., "Antioxidant capacity of extract from edible flowers of Prunus mume in China and its active components", Food Science and Technology, 42(2), pp.477~482 (2009)
  45. Siddhuraju, P., "Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp", Food chemistry, 79(1), pp.61~67 (2002)
  46. Swain, T., "The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents", Journal of the Science of Food and Agriculture, 10(1), pp.63~68 (1959)
  47. Thao, N.L.T., "Effect of ethanol on the anthocyanin extraction from the purple rice of Vietnam", Journal of Food and Nution Sciences, 3(1-2), pp.45~48 (2015)
  48. Thongchai, W., "Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2, 2′-diphenyl-1-picrylhydrazyl assay", Food chemistry, 112(2), pp.494~499 (2009)
  49. Turkmen, N., "The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables", Food chemistry, 93(4), pp.713~718 (2005)
  50. Wang, W.D., "Degradation kinetics of anthocyanins in blackberry juice and concentrate", Journal of Food Engineering, 82, pp.271~275 (2007)
  51. Wiseman, H., "Dietary influences on membrane function: importance in protection against oxidative damage and disease", The Journal of Nutritional Biochemistry, 7(1), pp.2~15 (1996)
  52. Wu, S., "Inhibitory effects of blue honeysuckle (Lonicera caerulea L) on adjuvant-induced arthritis in rats: Crosstalk of anti-inflammatory and antioxidant effects", Journal of Functional Foods, 17, pp.514~523 (2015)
  53. Wu, T., "Honeysuckle anthocyanin supplementation prevents diet-induced obesity in C57BL", 6 mice, 2013, pp.4(11), 1654~1661
  54. Xiang, Z., "Scavenging and antioxidant properties of compound derived from chlorogenic acid in South-China honeysuckle", Food Science and Technology, 41(7), pp.1189~1203 (2008)
  55. Zhao, B., "Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease", Neurochemical Research, 34(4), pp.630~638 (2009)
  56. Zhao, L., "Antioxidant Activities and Major Bioactive Components of Consecutive Extracts from Blue Honeysuckle (Lonicera Caerulea L.) Cultivated in China", Journal of Food Biochemistry, 39(6), pp.653~662 (2015)
  57. Zhou, Y., "Natural Polyphenols for Prevention and Treatment of Cancer", Nutrients, 8(8) (2016)

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