숙성 은행 외종피 추출물의 항균 및 항산화 활성
Antioxidant and Antimicrobial Activities of Extracts of Ripened Ginko biloba Outer Seedcoat
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The present study was carried out to evaluate the antioxidant and antimicrobial activities of extracts (hot water, 50% ethanol and 70% ethanol) of dried ripened Ginko biloba outer seedcoat (RGBO). Microorganism used for exeriments was three gram-positive bacteria such as Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Propionibacterium acnes (P. acnes), and two gram-negative bacteria, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). The bioassay applied for determining the antimicrobial effects of RGBO extracts included the disc diffusion assay. Antimicrobial activities of RGBO extracts were observed in all microbial species except for E. coli. Especially ethanol extracts were higher activity than water extract against microorganism. Antioxidant activities of the RGBO extracts were determined by DPPH and ABTS radical scavenging assays and SOD-like activity. The results indicated that antioxidant activities (DPPH, ABTS scavenging and SOD-like activities) were higher in water extract (DPPH: < 100 ug/ml, ABTS: < 500 ug/ml, SOD-like: 250 ug/ml) of RGBO. The order of antioxidant activities in RGBO extracts was water extract > 50% ethanol extract > 70% ethanol extract. These data suggest that extract of RGBO have antimicrobial and antioxidant activities. Therefore, it is considered that Ginko Biloba research range and selection of functional material can broden.
Abstract
The present study was carried out to evaluate the antioxidant and antimicrobial activities of extracts (hot water, 50% ethanol and 70% ethanol) of dried ripened Ginko biloba outer seedcoat (RGBO). Microorganism used for exeriments was three gram-positive bacteria such as Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Propionibacterium acnes (P. acnes), and two gram-negative bacteria, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). The bioassay applied for determining the antimicrobial effects of RGBO extracts included the disc diffusion assay. Antimicrobial activities of RGBO extracts were observed in all microbial species except for E. coli. Especially ethanol extracts were higher activity than water extract against microorganism. Antioxidant activities of the RGBO extracts were determined by DPPH and ABTS radical scavenging assays and SOD-like activity. The results indicated that antioxidant activities (DPPH, ABTS scavenging and SOD-like activities) were higher in water extract (DPPH: < 100 ug/ml, ABTS: < 500 ug/ml, SOD-like: 250 ug/ml) of RGBO. The order of antioxidant activities in RGBO extracts was water extract > 50% ethanol extract > 70% ethanol extract. These data suggest that extract of RGBO have antimicrobial and antioxidant activities. Therefore, it is considered that Ginko Biloba research range and selection of functional material can broden.
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