Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
BACKGROUND/OBJECTIVE: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 μg/mL. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with 300 μg/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 μg/mL of HBC and HBK (P < 0.01). Treatment with 300 μg/mL of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with 300 μg/mL of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.
Abstract
BACKGROUND/OBJECTIVE: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 μg/mL. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with 300 μg/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 μg/mL of HBC and HBK (P < 0.01). Treatment with 300 μg/mL of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with 300 μg/mL of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.
참고문헌 (40)
- Yang BY, "Protective effect of procyanidin B2 against CCl4-induced acute liver injury in mice", Molecules, 20, pp.12250~12265 (2015)
- Lu Y, "Protective effect of wedelolactone against CCl4-induced acute liver injury in mice", Int Immunopharmacol, 34, pp.44~52 (2016)
- Sun H, "The protective role of hydrogen-rich saline in experimental liver injury in mice", J Hepatol, 54, pp.471~480 (2011)
- Berasain C, "Inflammation and liver cancer : new molecular links", Ann N Y Acad Sci, 1155, pp.206~221 (2009)
- Zhao H, "Protective effect of anthocyanin from Lonicera caerulea var. edulis on radiationinduced damage in mice", Int J Mol Sci, 13, pp.11773~11782 (2012)
- Petersen F, "The effects of polyunsaturated fatty acids and antioxidant vitamins on atrial oxidative stress, nitrotyrosine residues, and connexins following extracorporeal circulation in patients undergoing cardiac surgery", Mol Cell Biochem, 433, pp.27~40 (2017)
- Szajdek A, "Bioactive compounds and health-promoting properties of berry fruits : a review", Plant Foods Hum Nutr, 63, pp.147~156 (2008)
- Svarcova I, "Berry fruits as a source of biologically active compounds : the case of Lonicera caerulea", Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 151, pp.163~174 (2007)
- Palíková I, "Protectivity of blue honeysuckle extract against oxidative human endothelial cells and rat hepatocyte damage", J Agric Food Chem, 57, pp.6584~6589 (2009)
- Jin XH, "Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo", Exp Eye Res, 82, pp.860~867 (2006)
- Zdarilová A, "Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts", Food Chem Toxicol, 48, pp.1555~1561 (2010)
- Jurgoński A, "An anthocyanin-rich extract from Kamchatka honeysuckle increases enzymatic activity within the gut and ameliorates abnormal lipid and glucose metabolism in rats", Nutrition, 29, pp.898~902 (2013)
- Park SI, "Therapeutic effects of blue honeysuckle on lesions of hyperthyroidism in rats", Am J Chin Med, 44, pp.1441~1456 (2016)
- Chen L, "Phytochemical properties and antioxidant capacities of various colored berries", J Sci Food Agric, 94, pp.180~188 (2014)
- Svobodová A, "Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes", Arch Dermatol Res, 300, pp.225~233 (2008)
- Vostálová J, "Lonicera caerulea fruits reduce UVA-induced damage in hairless mice", J Photochem Photobiol B, 128, pp.1~11 (2013)
- Choi JY, "Protective effect of the fruit hull of Gleditsia sinensis on LPS-induced acute lung injury is associated with Nrf2 activation", Evid Based Complement Alternat Med, 2012, pp.974713~ (2012)
- Kim J, "A protective role of nuclear factor-erythroid 2-related factor-2(Nrf2)in inflammatory disorders", Mutat Res, 690, pp.12~23 (2010)
- Wu PS, "Anti-inflammatory activity of 8-hydroxydaidzein in LPS-stimulated BV2 microglial cells via activation of Nrf2-antioxidant and attenuation of Akt", NF-κB-inflammatory signaling pathways, as well as inhibition of COX-2activity, 2018, pp.66, 5790~5801
- Bueno-Silva B, "Brazilian red propolis effects on peritoneal macrophage activity : nitric oxide, cell viability, pro-inflammatory cytokines and gene expression", J Ethnopharmacol, 207, pp.100~107 (2017)
- Kim DO, "Quantification of polyphenolics and their antioxidant capacity in fresh plums", J Agric Food Chem, 51, pp.6509~6515 (2003)
- Abdallah H, "Isolation of major phenolics from Launaea spinosa and their protective effect on HepG2 cells damaged with t-BHP", Pharm Biol, 54, pp.536~541 (2016)
- 최경희, "국내산 아로니아의 이화학 분석 및 항산화 활성", 한국식품영양과학회지, 44(8), pp.1165~1171 (2015), 한국식품영양과학회
- 홍승범, "위도가 다른 재배지역에서 생육한 검정콩의 안토시아닌 및 이소플라본 함량 변이", 한국환경농학회지, 29(2), pp.129~137 (2010), 한국환경농학회
- Surh YJ, "Cancer chemoprevention with dietary phytochemicals", Nat Rev Cancer, 3, pp.768~780 (2003)
- Kensler TW, "Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway", Annu Rev Pharmacol Toxicol, 47, pp.89~116 (2007)
- Zhang H, "Acrolein induces heme oxygenase-1 through PKC-δ and PI3K in human bronchial epithelial cells", Am J Respir Cell Mol Biol, 38, pp.483~490 (2008)
- Apopa PL, "Phosphorylation of Nrf2 in the transcription activation domain by casein kinase 2(CK2)is critical for the nuclear translocation and transcription activation function of Nrf2 in IMR-32neuroblastoma cells", J Biochem Mol Toxicol, 22, pp.63~76 (2008)
- Rushworth SA, "Role of protein kinase C δ in curcumin-induced antioxidant response element-mediated gene expression in human monocytes", Biochem Biophys Res Commun, 341, pp.1007~1016 (2006)
- Zimmermann K, "Activated AMPK boosts the Nrf2", HO-1 signaling axis--A role for the unfolded protein response, 2015, pp.88, 417~426
- Wang X, "Seabuckthorn berry polysaccharide extracts protect against acetaminophen induced hepatotoxicity in mice via activating the Nrf-2", HO-1-SOD-2 signaling pathway, 2018, pp.38, 90~97
- Dinkova-Kostova AT, "Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2enzymes that protect against carcinogens and oxidants", Proc Natl Acad Sci U S A, 99, pp.11908~11913 (2002)
- Lu SC, "Regulation of glutathione synthesis", Mol Aspects Med, 30, pp.42~59 (2009)
- Gozzelino R, "Mechanisms of cell protection by heme oxygenase-1", Annu Rev Pharmacol Toxicol, 50, pp.323~354 (2010)
- Poss KD, "Reduced stress defense in heme oxygenase 1-deficient cells", Proc Natl Acad Sci U S A, 94, pp.10925~10930 (1997)
- Yet SF, "Absence of heme oxygenase-1 exacerbates atherosclerotic lesion formation and vascular remodeling", FASEB J, 17, pp.1759~1761 (2003)
- Franklin CC, "Structure, function, and post-translational regulation of the catalytic and modifier subunits of glutamate cysteine ligase", Mol Aspects Med, 30, pp.86~98 (2009)
- Ross D, "NAD(P)H : quinone oxidoreductase 1(NQO1) : chemoprotection, bioactivation, gene regulation and genetic polymorphisms", Chem Biol Interact, 129, pp.77~97 (2000)
- Somani SM, "Interaction of exercise and ethanol on antioxidant enzymes in brain regions of the rat", Alcohol, 13, pp.603~610 (1996)
- Su D, "Phenolic-rich lychee (Litchi chinensis Sonn.) pulp extracts offer hepatoprotection against restraint stress-induced liver injury in mice by modulating mitochondrial dysfunction", Food Funct, 7, pp.508~515 (2016)
