고려인삼 신품종 ‘천량’ 특이적 DNA 판별 마커 개발
A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers
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초록
This study describes the efficient method for the discrimination of ‘Cheonryang’ in Panax ginseng Meyerusing a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminatingKorean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019,MFGp 0248, and successful identification of ‘Cheonryang’ was achieved from out of 11 Korean ginseng cultivars. Two differentsizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the samesize of amplified DNAs (389 bp), but ‘Cheonryang’ showed a different size. Thus ‘Cheonryang’ can be efficiently distinguishedfrom the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizesof DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shownin five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified inthe other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of ‘Cheonryang’ cultivar from alarge number of samples.
Abstract
This study describes the efficient method for the discrimination of ‘Cheonryang’ in Panax ginseng Meyerusing a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminatingKorean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019,MFGp 0248, and successful identification of ‘Cheonryang’ was achieved from out of 11 Korean ginseng cultivars. Two differentsizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the samesize of amplified DNAs (389 bp), but ‘Cheonryang’ showed a different size. Thus ‘Cheonryang’ can be efficiently distinguishedfrom the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizesof DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shownin five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified inthe other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of ‘Cheonryang’ cultivar from alarge number of samples.
참고문헌 (25)
- Bang KH, "Construction of genomic DNA library of Korean ginseng(Panax ginseng C. A. Meyer)and development of sequence-tagged sites", Biological and Pharmaceutical Bulletin, 33, pp.1579~1588 (2010)
- 방경환, "SSR 마커를 이용한 고려인삼 품종 판별기술 개발", 한국약용작물학회지, 19(3), pp.185~190 (2011), 한국약용작물학회
- Bernet GP, "Applicability of molecular markers in the context of the protection of new varieties of cucumber", Plant Breeding, 122, pp.146~152 (2003)
- Blake TK, "STS-PCR markers appropriate for wheat-barley introgression", Theoretical and Applied Genetics, 93, pp.826~832 (1996)
- Cooke RJ, "Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci", Euphytica, 132, pp.331~341 (2003)
- Erpelding JE, "Transfer of sequence tagged site PCR markers between wheat and barley", Genome, 39, pp.802~810 (1996)
- Hua S, "A simple and rapid technique for the authentication of the ginseng cultivar, Yunpoong, using an SNP marker in a large sample of ginseng leaves", Gene, 487, pp.75~79 (2011)
- 김율호, "STS-CAPS 마커 개발을 통한 110개 한국 콩 품종 판별", 한국육종학회지, 44(3), pp.258~272 (2012), 한국육종학회
- 조익현, "STS 마커를 이용한 고려인삼 품종 및 육성계통 판별", 한국약용작물학회지, 21(5), pp.353~360 (2013), 한국약용작물학회
- 조익현, "미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구", 한국약용작물학회지, 21(2), pp.91~96 (2013), 한국약용작물학회[DOI]
- Gi-Jun Kim, "Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars", 원예과학기술지, 29(3), pp.240~246 (2011), 한국원예학회
- 김영창, "염류 저항성 다수성 인삼 신품종 ‘천량’", 한국육종학회지, 45(4), pp.434~439 (2013), 한국육종학회[DOI]
- Komori T, "A simple method to control the seed purity of japonica hybrid rice varieties using PCR-based markers", Plant Breeding, 123, pp.549~553 (2004)
- 권영선, "AFLP 분석을 이용한 무와 배추의 품종식별", 한국육종학회지, 35(4), pp.319~328 (2003), 한국육종학회
- Lee OR, "Authentication of medicinal plants by SNP-based multiplex PCR", Methods in Molecular Biology, 862, pp.135~147 (2012)
- Seung Jae Lee, "Diversity and Varietal Classification of Hibiscus syriacus L. with the Heterogeneity within Retrotransposon-like Elements", Molecules and Cells, 13(3), pp.362~368 (2002), 한국분자세포생물학회
- Lefebvre V, "Evaluation of genetic distances between pepper inbred lines for cultivar protection purposes : Comparison of AFLP, RAPD and phenotypic data", Theoretical and Applied Genetics, 102, pp.741~750 (2001)
- Li X, "Development of STS and CAPS markers for identification of three tall larkspurs(Delphinium spp. )", Genome, 45, pp.229~235 (2002)
- Noil E, "Utilization of SSR and AFLP markers for the assessment of distinctness in durum wheat", Molecular Breeding, 22, pp.301~313 (2008)
- Olson M, "A common language for physical mapping of the human genome", Science, 245, pp.1434~1435 (1989)
- Rural Development Administration(RDA)., "Standard cultivation guidebook for good agricultural practice" (2009), Rural Development Administration
- Ming-Mao Sun, "SSR 마커에 의한 최근 육성 보급된 한국 벼 품종의 다양성과 품종 판별", 한국육종학회지, 41(2), pp.115~125 (2009), 한국육종학회
- Talbert LE, "Evaluation of"sequence-tagged-site"PCR products as molecular markers in wheat", Theoretical and Applied Genetics, 87, pp.789~794 (1994)
- Wang H, "A PCR-based SNP marker for specific authentication of Korean ginseng(Panax ginseng)cultivar"Chunpoong"", Molecular Biology Reports, 37, pp.1053~1057 (2010)
- Wang H, "Molecular authentication of Panax ginseng and ginseng products using robust SNP markers in ribosomal external transcribed spacer region", Journal of Pharmaceutical and Biomedical Analysis, 55, pp.972~976 (2011)