생장조절제와 프라이밍 처리에 의한 지치종자의 발아특성과 단백질 발현 양상에 관한 연구

Abstract

This study was conducted to investigate the quality of seeds, the germination rates and the days required forgermination, to examine the patterns of protein expressions during the germination and to improve the techniques of managingand storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvestingseeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. Thegermination rates, the days required for germination, and the protein expressions were examined with different colors ofseed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundredseed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in whitecolor of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storagedurations. There was no big differences on germination rates between storage temperatures. The plant growth regulator ofGA3 and Kinetin was affected to improve the seed germination. GA3 increased the seed germination clearly at 25 ppm level,while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germinationrate with increasing their levels, whereas KNO3 increased the germination until 100 mM level and then decreasedit with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bandsin the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showingmore and clearer bands were positively related to the germination rates which were different by seed colors, storage temperaturesand durations, and plant growth regulators and primings.