EST-SSR 마커를 이용한 인삼 품종과 육성계통의 유전적 다형성 및 유연관계 분석
Analysis of Genetic Polymorphism and Relationship of Korean Ginseng Cultivars and Breeding Lines using EST-SSR Marker
0
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
In this study, Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) analyses were used to clarify the genetic polymorphisms among Korean ginseng cultivars and breeding lines and to classify them into distinct genetic groups. Polymorphic and reproducible bands were produced by 14 primers out of total 30 primers used in this study. Fourteen ESTSSR loci generated a total of 123 bands. Amplified PCR products showed the highly reproducible banding patterns at 110~920 bp. The number of amplified bands for each EST-SSR primers ranged from 2 to 19 with a mean of 8.8 bands. P26and P35 primers showed 13 and 12 banding patterns, respectively. The number of alleles for each EST-SSR locus ranged from 1.67 to 2.00 with a mean of 1.878 alleles. P34 and P60 primers showed the highest and the lowest genetic polymorphism,respectively. Cluster analysis based on genetic similarity estimated by EST-SSR markers classified Korean cultivars and breeding lines into 4 groups. Group included Gopoong and Chunpoong and 9 breeding lines (55%), group included 2breeding lines (10%), group included 3 breeding lines (15%), group included Gumpoong and 3 breeding lines (20%). Consequently,the EST-SSR marker developed in this study may prove useful for the evaluation of genetic diversity and differentiation of Korean ginseng cultivars and breeding lines.
Abstract
In this study, Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) analyses were used to clarify the genetic polymorphisms among Korean ginseng cultivars and breeding lines and to classify them into distinct genetic groups. Polymorphic and reproducible bands were produced by 14 primers out of total 30 primers used in this study. Fourteen ESTSSR loci generated a total of 123 bands. Amplified PCR products showed the highly reproducible banding patterns at 110~920 bp. The number of amplified bands for each EST-SSR primers ranged from 2 to 19 with a mean of 8.8 bands. P26and P35 primers showed 13 and 12 banding patterns, respectively. The number of alleles for each EST-SSR locus ranged from 1.67 to 2.00 with a mean of 1.878 alleles. P34 and P60 primers showed the highest and the lowest genetic polymorphism,respectively. Cluster analysis based on genetic similarity estimated by EST-SSR markers classified Korean cultivars and breeding lines into 4 groups. Group included Gopoong and Chunpoong and 9 breeding lines (55%), group included 2breeding lines (10%), group included 3 breeding lines (15%), group included Gumpoong and 3 breeding lines (20%). Consequently,the EST-SSR marker developed in this study may prove useful for the evaluation of genetic diversity and differentiation of Korean ginseng cultivars and breeding lines.
참고문헌 (37)
- 방경환, "SSR 마커를 이용한 고려인삼 품종 판별기술 개발", 한국약용작물학회지, 19(3), pp.185~190 (2011), 한국약용작물학회
- 방경환, "SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석", 한국약용작물학회지, 19(5), pp.347~353 (2011), 한국약용작물학회
- Bang KH, "Construction of genomic DNA library of Korean ginseng (Panax ginseng C. A. Meyer) and development of sequence-tagged sites", Biological and Pharmaceutical Bulletin, 33, pp.1579~1588 (2010)
- Botstein D, "Construction of a genetic linkage map in man using restriction fragment length polymorphism", American Journal of Human Genetics, 32, pp.314~331 (1980)
- Chao S, "RFLP-based genetic maps of wheat homoeologous group-7 chromosomes", Theoretical and Applied Genetics, 78, pp.495~495 (1989)
- 서상덕, "RAPD 방법을 이용한 국내 수입 인삼(Panax ginseng C. A. meyer)의 다양성 분석", 한국약용작물학회지, 11(5), pp.377~384 (2003), 한국약용작물학회
- Dan NV, "Development and characterization of new microsatellite markers in Panax ginseng C. A. Meyer from BAC end sequences", Conserved Genetics, 11, pp.1223~1212 (2009)
- Fushimi H, "Application of PCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three Ginseng drugs", Biological and Pharmaceutical Bulletin, 20, pp.765~769 (1997)
- Ha WY, "Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD)", Journal of Agricultural and Food Chemistry, 50, pp.1871~1875 (2002)
- Hamada SR, "A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes", Proceedings of the National Academy of Sciences of the United States of America, 79, pp.6465~6469 (1982)
- Dong Su In, "Genetic Relationships of Panax Species by RAPD andISSR Analyses", 한국약용작물학회지, 13(5), pp.249~253 (2005), 한국약용작물학회
- Bo-Bae Kim, "Authentication of korean panax ginseng from Chinease Panax ginseng and Panax quinquefolius by AFLP analysis", Journal of Plant Biotechnology, 7(2), pp.81~86 (2005), 한국식물생명공학회
- ChangkyunKim, "Genetic Diversity and Relationship in Korean Ginseng(Panax schinseng) Based on RAPD Analysis", Genes & Genomics, 25(3), pp.181~188 (2003), 한국유전학회
- Kim OT, "Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences", Plant Biotechnology Reports, 1, pp.163~167 (2007)
- Komatsu K, "Phylogenetic analysis based on 18S rRNA gene and matK gene sequence of Panax vietnamensis and five related species", Planta Medica, 67, pp.461~465 (2001)
- Jei Wan Lee, "Development of DNA markers for identification of Korean ginseng cultivars (Panax ginseng C. A. Meyer)", 박사, pp.2010
- Lim W, "Utilization of RAPD markers to assess genetic diversity of wild populations of North American ginseng (Panax quinquefolius)", Planta Medica, 73, pp.71~76 (2007)
- Lim YP, "Survey of proper primers and genetic analysis of Korean ginseng (Panax ginseng C. A. Meyer) variants using the RAPD technique", Journal of Ginseng Research, 17, pp.153~158 (1993)
- McCouch SR, "Molecular mapping of rice chromosomes", Theoretical and Applied Genetics, 76, pp.815~819 (1988)
- Mullis K, "Specific enzymatic amplification of Dna in vitro: The polymerase chain reaction", Cold Spring Harbor Symposia on Quantitative Biology, 51, pp.263~273 (1986)
- Ngan F, "Molecular authentication of Panax species", Phytochemistry, 50, pp.787~791 (1999)
- Ogbonnaya FC, "Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in the wheat germplasm CN10955", Theoretical and Applied Genetics, 116, pp.891~902 (2008)
- Olsen M, "A common language for physical mapping of the human genome", Science, 245, pp.1434~1435 (1989)
- Paran I, "Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce", Theoretical and Applied Genetics, 85, pp.985~993 (1993)
- Rongwen J, "The use of microsatellite DNA markers for soybean genotype identification", Theoretical and Applied Genetics, 90, pp.43~48 (1995)
- Rural Development Administration, "Standard Cultivation Guidebook for Good Agricultural Practice", 47~117 (2009)
- Shaw PC, "Authentication of Panax species and their adulterants by random-primed polymerase chain reaction", Planta Medica, 61, pp.466~469 (1995)
- Young-Hun Shim, "Molecular Differentiation of Panax Species by RAPD Analysis", Archives of Pharmacal Research, 26(8), pp.601~605 (2003), 대한약학회
- Trautz D, "Simple sequence are ubiquitous repetitive components of eukaryotic genomes", Nucleic Acids Research, 12, pp.4127~4138 (1984)
- Um JY, "Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP", Biological and Pharmaceutical Bulletin, 24, pp.872~875 (2001)
- Vos P, "AFLP: a new techniques for DNA fingerprinting", Nucleic Acids Research, 23, pp.4407~4414 (1995)
- Wen J, "Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA", Molecular Phylogenetics and Evolution, 6, pp.167~177 (1996)
- Williams JGK, "DNA Polymorphism amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6531~6535 (1990)
- Wu KS, "Abundance, polymorphism and genetic mapping of microsatellites in rice", Molecular and General Genetics, 241, pp.225~235 (1993)
- Yang CJ, "Development of an EST-SSR marker in Panax ginseng", Chinese Journal of Agricultural Biotechnology, 5, pp.175~181 (2008)
- Yang DC, "Comparison of ITS (internal transcribed spacer) and 5.8S rDNA sequences among varieties and cultivars in Panax ginseng", Journal of Photoscience, 8, pp.55~60 (2001)
- POPGENE, the user-friendly shareware for population genetic analysis, "Yeh FC", Molecular Biology and Biotechnology Center. University of Alberta, ~ (1997)