미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구
Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species
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초록
This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as ‘AA’, ‘BA’ and ‘AB’,respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.
Abstract
This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as ‘AA’, ‘BA’ and ‘AB’,respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.
참고문헌 (24)
- Bakker FT, "Mitochondrial and chloroplast DNA-based phylogeny of Pelargonium(Geraniaceae)", American Journal of Botany, 4, pp.727~734 (2000)
- 방경환, "ISSRs 마크에 의한 고려 인삼의 분자적 인증과 유전적 다형현상", 생명과학회지, 14(3), pp.425~428 (2004), 한국생명과학회
- 방경환, "SSR 마커를 이용한 고려인삼 품종 판별기술 개발", 한국약용작물학회지, 19(3), pp.185~190 (2011), 한국약용작물학회
- 방경환, "SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석", 한국약용작물학회지, 19(5), pp.347~353 (2011), 한국약용작물학회
- 방경환, "EST-SSR 마커를 이용한 인삼 품종과 육성계통의 유전적 다형성 및 유연관계 분석", 한국약용작물학회지, 20(4), pp.277~285 (2012), 한국약용작물학회[DOI]
- Choi HK, "A phylogenetic analysis of Panax(Araliaceae): Integrating cpDNA restriction site and nuclear rDNA ITS sequence data", Plant Systematics and Evolution, 224, pp.109~120 (2000)
- Duminil J, Pemonge MH and Petit RJ., "A set of 35 consensus primer pairs amplifying genes and introns of plant mitochondrial DNA", Molecular Ecology Notes, 4, pp.428~430 (2002)
- Guo YL, "Molecular phylogeny of Oryzeae(Poaceae) based on DNA sequences from chloroplast, mitochondrial, and nuclear genomes", American Journal of Botany, 99, pp.1548~1558 (2005)
- Ha WY, "Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism(AFLP) and directed amplification of minisatellite region DNA(DAMD)", Journal of Agricultural and Food Chemistry, 7, pp.1871~1875 (2002)
- Hu SY., "The genus Panax(ginseng) in Chinese medicine", Economic Botany, 30, pp.11~28 (1976)
- 김율호, "STS-CAPS 마커 개발을 통한 110개 한국 콩 품종 판별", 한국육종학회지, 44(3), pp.258~272 (2012), 한국육종학회
- Dong Su In, "Genetic Relationships of Panax Species by RAPD andISSR Analyses", 한국약용작물학회지, 13(5), pp.249~253 (2005), 한국약용작물학회
- Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences, "Kim OT", Plant Biotechnology Reports, 163~167 (2007)
- Lee C, "Phylogeny of Panax using trnC-trnD intergenic region and the utility of trnC-trnD in interspecific studies of plants", Molecular Phylogenetics Evolution, 31, pp.894~903 (2004)
- McClean PE, "Mitochondrial DNA sequence divergence among lycopersicon and related Solanum species", Genetics, 3, pp.649~667 (1986)
- Merckx V, "Phylogeny and evolution of Burmanniaceae(Dioscoreales) based on nuclear and mitochondrial data", American Journal of Botany, 11, pp.1684~1698 (2006)
- Ngan F, "Molecular authentication of Panax species", Phytochemistry, 50, pp.787~791 (1999)
- Park MJ, "Molecular identification of Korean ginseng by amplification refractory mutation system-PCR", Food Research International, 5, pp.568~574 (2006)
- Schaefer H, "Phylogenetic relationships in the order Cucurbitales and a new classification of the gourd family(Cucurbitaceae)", Taxonomy, 60, pp.122~138 (2011)
- Young-HunShim, "Molecular Differentiation of Panax Species by RAPD Analysis", Archives of Pharmacal Research, 26(8), pp.601~605 (2003), 대한약학회
- Shim YH, "Identification of Panax species in the herbal medicine preparations using gradient PCR method", Biological and Pharmaceutical Bulletin, 28, pp.671~676 (2005)
- Um JY, "Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP", Biological and Pharmaceutical Bulletin, 24, pp.872~875 (2001)
- Wang H, "Molecular identification of the Korean ginseng cultivar “Chunpoong” using the mitochondrial nad7 intron 4 region", Mitochondrial DNA, 20, pp.41~45 (2009)
- Wen J, "Phylogeny and biogeography of Panax L.(the ginseng genus, Araliaceae): Inferences from ITS sequences of nuclear ribosomal DNA", Molecular Phylogenetics and Evolution, 6, pp.167~177 (1996)