분자지표를 이용한 고려인삼의 유전적 특성 비교
Comparative Genetic Characteristics of Korean Ginseng using DNA Markers
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초록
The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study,18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei’s genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.
Abstract
The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study,18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei’s genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.
참고문헌 (32)
- Bai D, "Genetic diversity in north America ginseng(Panax quinquefolius L. )grown in Ontario detected by RAPD analysis", Genome, 40, pp.111~115 (1997)
- 방경환, "해부형태적 특징과 SCAR Marker를 이용한 백출의 기원식물 판별", 한국약용작물학회지, 12(1), pp.53~59 (2004), 한국약용작물학회
- Bang KH, "Construction of genomic DNA library of Korean ginseng(Panax ginseng C. A. Meyer) and development of sequence-tagged sites", Biological and Pharmaceutical Bulletin, 33, pp.1579~1588 (2010)
- 방경환, "SSR 마커를 이용한 고려인삼 품종 판별기술 개발", 한국약용작물학회지, 19(3), pp.185~190 (2011), 한국약용작물학회
- 방경환, "EST-SSR 마커를 이용한 인삼 품종과 육성계통의 유전적 다형성 및 유연관계 분석", 한국약용작물학회지, 20(4), pp.277~285 (2012), 한국약용작물학회[DOI]
- Bernet GP, "Applicability of molecular markers in the context of protection of new varieties of cucumber", Plant Breeding, 122, pp.146~152 (2003)
- Boehm CL, "Organization of American and Asian ginseng germplasm using randomly amplified polymorphic DNA(RAPD)marker", Journal of the American Society for Horticultural Science, 124, pp.252~256 (1999)
- Cui XM, "Authentication of Panax notoginseng by 5S-rRNA spacer domain and random amplified polymorphic DNA(RAPD)analysis", Planta Medica, 69, pp.584~586 (2003)
- Devos KM, "The use of random amplified polymorphic DNA markers in wheat", Theoretical and Applied Genetics, 84, pp.567~572 (1992)
- Hamada SR, "A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes", Proceedings of National Academy of Science of the United States of America, 79, pp.6465~6469 (1982)
- 강희완, "Fingerprinting of Diverse Genomes Using PCR with Universal Rice Primers Generated from Repetitive Sequence of Korean Weedy Rice", Molecules and Cells, 13(2), pp.281~287 (2002), 한국분자세포생물학회
- Kang HW and Kang KK., "Genomic characterization of Oryza species-specific CACTA-like transposon element and its application for genomic fingerprinting of rice varieties", MOLECULAR BREEDING, 21, pp.283~292 (2008), SPRINGER
- Nam Hoon Kim, "EST-SSR Marker Sets for Practical Authentication of All Nine Registered Ginseng Cultivars in Korea", Journal of Ginseng Research, 36(3), pp.298~307 (2012), 고려인삼학회
- Kwon WS, "Comparisons of growth, crude saponin, ginsenosides, and anthocyanins in superior lines of Panax ginseng C. A. Meyer", Journal of Ginseng Research, 23, pp.219~228 (1991)
- Kwon WS, "Breeding process and characteristics of Yunpoong, a new variety", Journal of Ginseng Research, 24, pp.1~751 (2000)
- Kwon WS, "Characteristics of flowering and fruiting in new varieties and lines of Panax ginseng C. A. Meyer", Journal of Ginseng Research, 25, pp.41~44 (2001)
- 권우생, "인삼(Panax ginseng C.A. Meyer) 신품종 고풍의 육성경과 및 생육특성", Journal of Ginseng Research, 27(2), pp.8~91 (2003), 고려인삼학회
- Jei-Wan Lee, "Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)", Journal of Ginseng Research, 35(1), pp.52~59 (2011), 고려인삼학회
- Lewontin RC., "The apportionment of human diversity", Evolutionary Biology, 6, pp.381~398 (1972)
- Lim W, "Utilization of RAPD markers to assess genetic diversity of wild populations of north American ginseng(Panax quinquefolium)", Planta Medica, 73, pp.71~76 (2007)
- Ministry of Food and Drug Safety(MFDS)., "State of health functional foods in 2012", Ministry of Food and Drug Safety, ~ (2013)
- Mullis KB, "Specific enzymatic amplification of DNA in vitro : The polymerase chain reaction", Cold Spring Harbor Symposia on Quantitative Biology, 51, pp.263~273 (1986)
- 임순영, "인삼(Panax ginsneg C.A. Meyer) 수집종의 주요 특성 및 유연관계 분석", 한국육종학회지, 42(5), pp.488~494 (2010), 한국육종학회
- Rural Development Administration(RDA)., "Standard cultivation guidebook for good agricultural practice", Rural Development Administration, 47~117 (2009)
- 서상덕, "RAPD 방법을 이용한 국내 수입 인삼(Panax ginseng C. A. meyer)의 다양성 분석", 한국약용작물학회지, 11(5), pp.377~384 (2003), 한국약용작물학회
- Young-HunShim, "Molecular Differentiation of Panax Species by RAPD Analysis", Archives of Pharmacal Research, 26(8), pp.601~605 (2003), 대한약학회
- Tautz D, "Simple sequences are ubiquitous repetitive components of eukaryotic genomes", Nucleic Acids Research, 12, pp.4127~4138 (1984)
- Williams JGK, "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6531~6535 (1990)
- Young Hua Xu, "Genetic Diversity and Genetic Structures in Ginseng Landraces (Cultivars) by SRAP Analysis", 한국약용작물학회지, 18(3), pp.180~185 (2010), 한국약용작물학회
- Yang CJ, "Development of an EST-SSR marker in Panax ginseng", Chinese Journal of Agricultural Biotechnology, 5, pp.175~181 (2008)
- 양덕춘, "PCR-aided RFLP기술을 이용한 인삼의 DNA분석", Journal of Ginseng Research, 27(3), pp.10~150 (2003), 고려인삼학회
- Yeh FC, "POPGENE, the user-friendly shareware for population genetic analysis", Molecular Biology and Biotechnology Center, University of Alberta (1997)