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갯방풍 지상부 용매 분획물의 비표적 대사체 프로파일링 및 항산화 활성

Antioxidant Activity and Non-Targeted Metabolic Profiling of Solvent Fractions of Glehnia littoralis Shoots

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Background: The soft petiole of the Glehnia littoralis shoot is used as a raw material for food. It contains specific ingredients such as polyacetylene and coumarin. However, basic research considering their safety verification, efficacy, and ingredient identification is needed. Methods and Results: In this study, GC/MS compound profiling and LC/UVD were performed to evaluate the antioxidant activity of G. littoralis shoot extract fractions, and to identify the compounds contained therein. Total phenol and flavonoid content as well as antioxidant activity were maximum in the ethyl acetate layer, and a significant correlation was confirmed between total phenol content and antioxidant activity. GC/MS compound profiling, revealed a total of more than 40 compounds, and active ingredients such as coumarin-based phellopterin unique to G. littoralis and polyacetylene-based falcarinol (panaxinol) were qualitatively analyzed. The highest coumarin content was found in the ethyl acetate layer. Conclusions: In this study, the antioxidant activity and compounds contained in the shoot (soft petiole) fractions of G. littoralis were confirmed. These basic data may contribute to expanding the material value of G. littoralis in the future.

Abstract

Background: The soft petiole of the Glehnia littoralis shoot is used as a raw material for food. It contains specific ingredients such as polyacetylene and coumarin. However, basic research considering their safety verification, efficacy, and ingredient identification is needed. Methods and Results: In this study, GC/MS compound profiling and LC/UVD were performed to evaluate the antioxidant activity of G. littoralis shoot extract fractions, and to identify the compounds contained therein. Total phenol and flavonoid content as well as antioxidant activity were maximum in the ethyl acetate layer, and a significant correlation was confirmed between total phenol content and antioxidant activity. GC/MS compound profiling, revealed a total of more than 40 compounds, and active ingredients such as coumarin-based phellopterin unique to G. littoralis and polyacetylene-based falcarinol (panaxinol) were qualitatively analyzed. The highest coumarin content was found in the ethyl acetate layer. Conclusions: In this study, the antioxidant activity and compounds contained in the shoot (soft petiole) fractions of G. littoralis were confirmed. These basic data may contribute to expanding the material value of G. littoralis in the future.

참고문헌 (44)

  1. Budzynska B, "Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice", Psychopharmacology, 232, pp.931~942 (2015)
  2. Chandra S, "Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops : A comparative study", Evidence-Based Complementary and Alternative Medicine, 2014, pp.253875~ (2014)[DOI]
  3. Chang WS, "Structure-activity relationship of coumarins in xanthine oxidase inhibition", Anticancer Research, 15, pp.1969~1973 (1995)
  4. 최종일, "칠면초 분획물의 항산화 활성", 한국식품영양과학회지, 38(2), pp.131~135 (2009), 한국식품영양과학회
  5. 최선주, "사과꽃잎 추출물의 용매 분획으로부터 기능성 소재의 탐색", KSBB Journal, 26(2), pp.165~171 (2011), 한국생물공학회
  6. 추병길, "갯방풍 자생지의 식생구조 및 군락특성에 관한 연구", 한국환경복원기술학회지, 11(6), pp.38~48 (2008), 한국환경복원기술학회
  7. Clarke G, "High correlation of 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest", Antioxidants, 2013, pp.2~ (2013)[DOI]
  8. de la Cruz JF, "Glehnia littoralis root extract induces G0", G1 phase cell cycle arrest in the MCF-7 human breast cancer cell line, 2015, pp.16, 8113~8117
  9. Esmaeili AK, "Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pratense L. (Red Clover)", BioMed Research International, 2015, pp.643285~ (2015)[DOI]
  10. Hacke ACM, "Ethyl acetate fraction of Cymbopogon citratus as a potential source of antioxidant compounds", New Journal of Chemistry, 42, pp.3642~3652 (2018)
  11. Hanzhi L, "Nutrient sensing and the oxidative stress response", Trends in Endocrinology and Metabolism, 28, pp.449~460 (2017)
  12. Helbling-Leclerc A, "Beyond DNA repair and chromosome instability-Fanconi anaemia as a cellular senescence-associated syndrome", Cell Death and Differentiation, 28, pp.1159~1173 (2021)
  13. 장귀영, "열처리와 추출방법에 따른 몇 가지 약초의 항산화 활성", 한국식품영양과학회지, 41(7), pp.914~920 (2012), 한국식품영양과학회[DOI]
  14. 전상민, "참취 추출물과 용매분획물의 항산화 활성", 한국식품영양과학회지, 41(9), pp.1197~1204 (2012), 한국식품영양과학회
  15. Kasote DM, "Significance of antioxidant potential of plants and its relevance to therapeutic applications", International Journal of Biological Sciences, 11, pp.982~991 (2015)
  16. 김보민, "배초향 에틸아세테이트 분획의 산화방지 및 항염증 활성", 한국식품과학회지, 49(3), pp.331~337 (2017), 한국식품과학회[DOI]
  17. 방미희, "국산 건대추의 외형 등급별 품질특성 연구", 한국약용작물학회지, 28(6), pp.455~462 (2020), 한국약용작물학회
  18. 김경철, "굴거리 열매 추출물의 폴리페놀 함량과 항산화 활성", 공학기술논문지, 12(4), pp.149~155 (2019), 공학기술연구원[DOI]
  19. Kim SJ, "Screening for superoxide dismutase-like compounds and its activators in extracts of fruits and vegetables", Bioscience, Biotechnology, and Biochemistry, 58, pp.2263~2265 (1994)
  20. 윤성탁, "갯방풍의 지리적 분포와 자생지 특성", 한국약용작물학회지, 13(4), pp.171~177 (2005), 한국약용작물학회
  21. 김원백, "산복사나무(Prunus davidiana (Carriere) Franch.) 열매 분획 추출물의 항산화 및 미백 효능", 한국식품영양과학회지, 41(10), pp.1363~1370 (2012), 한국식품영양과학회
  22. Kontogiorgis CA, "Synthesis and antiinflammatory activity of coumarin derivatives", Journal of Medicinal Chemistry, 48, pp.6400~6408 (2005)
  23. 권경철, "잣나무 잎 추출물의 생리활성 및 주름개선 효능", 한국약용작물학회지, 29(2), pp.117~123 (2021), 한국약용작물학회[DOI]
  24. 권강무, "구증구포 도라지 Ethyl Acetate 분획물의 예쁜 꼬마선충 내의 항산화 효과", 생약학회지, 51(4), pp.325~331 (2020), 한국생약학회[DOI]
  25. 이병국, "갯방풍(Glehnia littoralis Schmidt et Miquiel)에서 캘러스 유도 및 식물체 재분화에 미치는 생장조절제의 효과", 한국자원식물학회지, 17(2), pp.153~160 (2004), 한국자원식물학회
  26. 이민수, "우리나라 해안에 서식하는 염생식물의 분포 특징", 바다, 24(1), pp.139~159 (2019), 한국해양학회[DOI]
  27. 이연리, "Antioxidative Activities of the Ethyl Acetate Fraction from Heated Onion (Allium cepa)", Food Science and Biotechnology, 16(6), pp.1041~1045 (2007), 한국식품과학회
  28. Li H, "Effects of the Chinese herb component phellopterin on the increase in cytosolic free calcium in PC12 cells", Drug Development Research, 68, pp.79~83 (2007)
  29. Marklund S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, 47, pp.469~474 (1974)
  30. Micco RD, "Cellular senescence in ageing : From mechanisms to therapeutic opportunities", Nature Reviews Molecular Cell Biology, 22, pp.75~95 (2020)
  31. Min BM, "On the accumulation of minerals with the plant species in a reclaimed land", Korean Journal of Ecology, 13, pp.9~18 (1990)
  32. Ministry of Food and Durg Safety(MFDS), "Food code 2021", Ministry of Food and Drug Safety, 1~17 (2021)
  33. Naranjo JD, "Regenerative medicine approaches for age-related muscle loss and sarcopenia : A mini-review", Gerontology, 63, pp.580~589 (2017)
  34. 오영선, "층층갈고리둥굴레 (Polygonatum sibiricum Redoute) 뿌리줄기와 잎의 증숙 시간과 증숙 온도에 따른 생리활성 변이 연구", 한국약용작물학회지, 28(5), pp.331~338 (2020), 한국약용작물학회[DOI]
  35. Ozgen M, "Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(abts) method to measure antioxidant capacity of Selected small fruits and comparison to ferric reducing antioxidant power(FRAP) and 2,2'-diphenyl-1-picrylhydrazyl(DPPH) methods", Journal of Agricultural and Food Chemistry, 54, pp.1151~1157 (2006)
  36. Park JH, "Clinical applications of antioxidants", Hanyang Medical Reviews, 33, pp.130~136 (2013)
  37. Park JW., "Antioxidative activities and activecompounds in the roots of turkestan rose(Rosa rugosa Thunberg var. rugosa)", Ph.D., pp.2006
  38. 박미혜, "Analysis of Antioxidant and Anti-Inflammatory Activities of Solvent Fractions from Rhynchosia nulubilis Cultivated with Ganoderma lucidum Mycelium", Preventive Nutrition and Food Science, 22(4), pp.365~371 (2017), 한국식품영양과학회[DOI]
  39. Ryu SY, "Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi", Korean Jounrnal of Pesticide Science, 5, pp.26~35 (2001)
  40. Seo YK, "Study on the components of Glehniae Radix", Korean Journal of Pharmacognosy, 7, pp.233~235 (1976)
  41. Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent", Methods in Enzymology, 299, pp.152~178 (1999)
  42. 엄영란, "염생식물 갯방풍의 화학적 성분연구", 대한화학회지, 54(6), pp.701~706 (2010), 대한화학회
  43. Yoon TS, "Anti-inflammatory activity of Methylene chloride fraction from Glehnia littoralis extract via suppression of NF-κB and mitogen-activated protein kinase activity", Journal of Pharmacological Sciences, 112, pp.46~55 (2010)
  44. Zargoosh Z, "Effects of ecological factors on the antioxidant potential and total phenol content of Scrophularia striata Boiss", Scientific Reports, 9, pp.16021~ (2019)[DOI]

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