해방풍 부위별 용매추출물의 항산화 활성 및 nitric oxide 생성 억제
Antioxidant activities of solvent extracts from different Glehnia Radix parts and their inhibitory effect against nitric oxide production in Raw 264.7 cell
2
조회
0
다운로드
17
인용
0
참고문헌
키워드
초록
In this study, we compared the antioxidant and immuno-modulatory effects of water (HR, HL, HS), 70% ethanol (ER, EL, ES), and 70% methanol (MR, ML, MS) extracts of different parts (root, leaf, and seed) from Glehnia Radix. Yields were 17.40-30.17% for water extract, 11.47-28.67% for 70% ethanol extract, and 10.73-30.57% for 70% methanol extract, respectively. The total polyphenol and flavonoid contents of EL were 10.79 g/100 g and 2.01 g/100 g, respectively. The DPPH and ABTS radical scavenging activities of EL at 1,000 µg/mL were 84.70% and 57.64%, respectively. The superoxide radical scavenging and ferric-reducing antioxidant power of EL at 1,000 µg/mL were 84.05% and 975.28 μM, respectively. Moreover, 70% ethanol and 70% methanol extracts of root from Glehnia Radix significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results suggest that 70% ethanol and 70% methanol extracts of Glehnia Radix leaf may be a useful functional food material in the food industry.
Abstract
In this study, we compared the antioxidant and immuno-modulatory effects of water (HR, HL, HS), 70% ethanol (ER, EL, ES), and 70% methanol (MR, ML, MS) extracts of different parts (root, leaf, and seed) from Glehnia Radix. Yields were 17.40-30.17% for water extract, 11.47-28.67% for 70% ethanol extract, and 10.73-30.57% for 70% methanol extract, respectively. The total polyphenol and flavonoid contents of EL were 10.79 g/100 g and 2.01 g/100 g, respectively. The DPPH and ABTS radical scavenging activities of EL at 1,000 µg/mL were 84.70% and 57.64%, respectively. The superoxide radical scavenging and ferric-reducing antioxidant power of EL at 1,000 µg/mL were 84.05% and 975.28 μM, respectively. Moreover, 70% ethanol and 70% methanol extracts of root from Glehnia Radix significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results suggest that 70% ethanol and 70% methanol extracts of Glehnia Radix leaf may be a useful functional food material in the food industry.
참고문헌 (35)
- Kim SY, "Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice", Kor J Pharmacogn, 30, pp.123~129 (1999)
- 조인숙, "장내 미생물 개선효과가 있는 약용식물소재 탐색", 한국약용작물학회지, 15(1), pp.26~29 (2007), 한국약용작물학회
- 신정혜, "한약재 열수추출물의 항산화 활성", 한국식품영양과학회지, 35(1), pp.7~14 (2006), 한국식품영양과학회
- Kang KJ, "A survey on housewives, consumption pattern and nutrition knowledge about vegetables", Korean J Dietary Culture, 10, pp.377~390 (1995)
- Ham SS, "Development of beverages drinks using mountain edible herbs", J Korean Soc Food Sci Nutr, 26, pp.92~97 (1997)
- 유아름, "생약 추출물의 RAW 264.7 세포를 이용한 면역증강 효과", 한국식품영양과학회지, 41(11), pp.1521~1527 (2012), 한국식품영양과학회
- Kim JH, "Active compounds from Glehniae Radix cum Rhizoma", MS, pp.2008
- Cho SI, "Pharmacological studies on Glehniae Radix and Arisaematis Rhizoma", Kor J Pharmacogn, 16, pp.50~51 (1985)
- Ng TB, "The antioxidant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostellaria heterophylla and Glehnia littoralis", J Ethnopharmacol, 93, pp.285~288 (2004)
- Kim DM, "Comparative study of anti-inflammatory effects on roots of Glehnia littoralis and Saposhnikovia divaricata", MS, pp.2006
- Chawla A, "Macrophage-mediated inflammation in metabolic disease", Nat Rev Immunol, 11, pp.738~749 (2011)
- Cencioni C, "Oxidative stress and epigenetic regulation in ageing and age-related diseases", Int Mol Sci, 14, pp.17643~17663 (2013)
- Sivaranjani N, "Role of reactive oxygen species and antioxidants in atopic dermatitis", J Clin Diagn Res, 7, pp.2683~2685 (2013)
- 임도연, "철쭉 가지 에탄올 추출물 및 용매별 분획물의 항산화 활성과Tyrosinase 저해 활성", 한국약용작물학회지, 21(6), pp.439~443 (2013), 한국약용작물학회[DOI]
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", AM J Enol Vitic, 16, pp.144~158 (1965)
- Jia Z, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Benzie IFF, "The ferric reducing ability of plasma(FRAP)as a measure of"antioxidant power"the FRAP assay", Anal Biochem, 239, pp.70~76 (1996)
- Oyaizu M, "Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr Diet, 44, pp.307~315 (1986)
- 성낙주, "머루 과피 용매추출물의 항산화성 및 아질산염 소거작용", 한국식품영양과학회지, 35(8), pp.961~966 (2006), 한국식품영양과학회
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent", Method Enzymol, 299, pp.152~177 (1999)
- 치용카이, "식용 및 약용버섯의 항산화 및 In vitro 항암 효과", 한국식품영양과학회지, 42(5), pp.655~662 (2013), 한국식품영양과학회
- Wang SY, "Antioxidant properties and phytochemical characteristics of extracts from Lactuca indica", J Agric Food Chem, 51, pp.1506~1512 (2003)
- 권하나, "산겨릅나무 추출물의 항산화 및 간 기능 보호효과", 한국식품영양과학회지, 37(11), pp.1389~1394 (2008), 한국식품영양과학회
- Seo YH, "Electron donationg ability and contents of phenolic compounds, tocopherols and carotenoids in waxy corn(Zea mays L. )", Korean J Food Sci Technol, 31, pp.581~585 (1999)
- Kim HK, "Changes of total polyphenol content and electron donating ability of Aster glehni extracts with different microwave-assisted extraction conditions", Korean J Food Sci Technol, 32, pp.1022~1028 (2000)
- 김형수, "어성초의 추출물에 따른 항산화력 연구", 한국산학기술학회논문지, 14(10), pp.5091~5096 (2013), 한국산학기술학회[DOI]
- Rice-Evans CA, "Structure antioxidant activity relationships of flavonoids and phenolic acid", Free Radic Biol Med, 20, pp.933~956 (1996)
- Stuehr DJ, "Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD-and FMN-containing flavoprotein", Proc Natl Acad Sci USA, 88, pp.7773~7777 (1991)
- McCartney-Francis N, "Supperession of arthritis by an inhibitor of nitric oxide synthase", J Exp Med, 178, pp.749~754 (1993)
- Weisz A, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine", Biochem J, 316, pp.209~215 (1996)
- Omata N, "Increased oxidative stress in childhood atopic dermatitis", Life Sci, 69, pp.223~228 (2001)
- Kim JN, "Effects of Sec-O-glucosylhamaudol, a compound from Peucedanum japonicum Thunb extract on the LPS-Stimulated RAW 264. 7 cell lines", MS, pp.2015