추출 용매에 따른 갯질경, 풀고사리, 방기 잎 추출물의 항산화 활성 비교 연구
Comparison of antioxidant activities of extracts of Limonium tetragonum, Gleichenia japonica, Sinomenium acutum leaves with different solvents
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초록
This study was designed to explore the antioxidant activities of water and ethanolextracts of Limonium tetragonum, Gleichenia japonica, and Sinomenium acutum leaves. Measurement of the total flavonoid and polyphenolic compounds showed that ethanol extracts hadhigher amounts than water extracts for all 3 plants. To estimate the antioxidant activities of leafextracts with different solvents, DPPH and ABTS assays were carried out. In both the DPPH andABTS assays for L. tetragonum, the antioxidant effect of water extract was slightly higher thanthat of ethanol extract at low concentration (12.5-50 μg/mL), but the opposite result was observedat 100 μg/mL. In the case of G. japonica, the ethanol extract showed higher antioxidant activitythan the water extract over the entire range of concentrations in both the DPPH and ABTS assays. For the S. acutum extracts, the ethanol extract showed a greater antioxidant effect than the waterextract over the entire concentration range by the ABTS method, but with the DPPH method thewater extract showed a greater effect than the ethanol extract in the concentration range, exceptfor the lowest concentration (12.5 μg/mL). HPLC was performed to analyze the polyphenoliccompounds in the 3 ethanol extracts with testing for 17 polyphenolic compounds. Severalpolyphenolic compounds were detected with the highest amount found for hesperidin. Theseresults imply the feasibility of the development of these leaf extracts as antioxidant agents.
Abstract
This study was designed to explore the antioxidant activities of water and ethanolextracts of Limonium tetragonum, Gleichenia japonica, and Sinomenium acutum leaves. Measurement of the total flavonoid and polyphenolic compounds showed that ethanol extracts hadhigher amounts than water extracts for all 3 plants. To estimate the antioxidant activities of leafextracts with different solvents, DPPH and ABTS assays were carried out. In both the DPPH andABTS assays for L. tetragonum, the antioxidant effect of water extract was slightly higher thanthat of ethanol extract at low concentration (12.5-50 μg/mL), but the opposite result was observedat 100 μg/mL. In the case of G. japonica, the ethanol extract showed higher antioxidant activitythan the water extract over the entire range of concentrations in both the DPPH and ABTS assays. For the S. acutum extracts, the ethanol extract showed a greater antioxidant effect than the waterextract over the entire concentration range by the ABTS method, but with the DPPH method thewater extract showed a greater effect than the ethanol extract in the concentration range, exceptfor the lowest concentration (12.5 μg/mL). HPLC was performed to analyze the polyphenoliccompounds in the 3 ethanol extracts with testing for 17 polyphenolic compounds. Severalpolyphenolic compounds were detected with the highest amount found for hesperidin. Theseresults imply the feasibility of the development of these leaf extracts as antioxidant agents.
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