미성숙 맥문동 열매 추출물의 항노화 및 항산화 활성

Abstract

Our study investigated the anti-wrinkle, whitening, astringent, antioxidant, cytotoxicity, and inhibition of nitric oxide (NO) inhibitory effects of immature Liriope platyphylla fruits as a cosmeceutical using the following extracts: (1) Hot water extract of L. platyphylla immature fruits (HWLIF) using a high pressure extractor; (2) Water extract of L. platyphylla immature fruits (WLIF) using a reflux extract; and (3) 80% ethanol extract of L. platyphylla immature fruits (ELIF) using a reflux extract. Polyphenol compounds were the highest in WLIF at 73.57 mg·TA/g. The highest amount of flavonoid compounds were detected in ELIF at 25.51 mg·QC/g. At a concentration of 2000 ㎍/mL, ELIF showed the highest collagenase inhibition rate of 35.28% and tyrosinase inhibition rate of 36.3%. The elastase inhibition activity was relatively high in WLIF at 24.76%, and the astringent effect was observed in HWLIF. The ABTS (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was over 64% for all the three extracts, with WLIF showing the highest activity at 69.03%. The ELIF exhibited a DPPH (1,1-Diphenyl-2- picrylhydrazyl) radical scavenging activity of 104.81%, which was higher than the positive control L-ascorbic acid (98.23%). Furthermore, ELIF showed the highest superoxide dismutase (SOD)-like activity at 42.85%. In the MTT (3-[4,5-dimethylythiazole-2-yl]-2,5 diphenyl tetrazolium bromide) assay in RAW264.7 macrophages, the cytotoxicity of the immature L. platyphylla fruits extracts were confirmed to be non-cytotoxic. When treated with 200 ㎍/mL MTT, the cell viability in ELIF decreased by approximately 15.7%. The inhibition rate of NO production showed that all extracts had an anti-inflammatory effect. The WLIF, treated with 200 ㎍/mL MTT, showed the best anti-inflammatory effect with approximately 30% reduction in NO production. In conclusion, the extracts from immature L. platyphylla fruits were not cytotoxic. The ethanol extract (ELIF) demonstrated the most potent anti-aging, antioxidant, and anti- inflammatory activities. Therefore, immature L. platyphylla fruits could be potentially used in recycling resources and as a natural functional ingredient in cosmoceuticals.