벌나무 추출물의 황생포도상구균에 대한 항균 효과와 항산화 활성
Antibacterial, Antioxidant Activities of Acer tegmentosum Maxim Ethanol Extract Against Staphylococcus aureus
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This study investigates the antibacterial and antioxidant activities of the ethanol extract of Acer tegmentosum Maxim (EATM) against Staphylococcus aureus. The total polyphenol and flavonoid content, 1,1-diphenyl-2- picryl hydrazyl (DPPH) and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging effect, and reducing power of the ferric reducing antioxidant power (FRAP) method were measured to verify the antioxidant activity of the EATM. The antibacterial activity against S. aureus using the EATM was verified by the paper disc method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The total polyphenol and flavonoid contents were 266.66 μg GAE/mg and 6.46 μg QE/mg, respectively. The DPPH and ABTS radical scavenging activity showed a concentration-dependent scavenging activity. The RC50 values of the EATM were 21.49 and 12.81 μg/mL, respectively. A FRAP analysis was conducted for evaluating the reducing power of the EATM and an efficacy of 0.73 ± 0.19 mM FeSO4 E/mg was observed. The antibacterial activity of EATM against S. aureus, determined using the paper disc method, showed an inhibitory ring of 3 mm at 2 mg. The MIC was confirmed at a concentration of ≥ 16 mg/mL, while the MBC was confirmed at 32 mg/mL. As the EATM shows antioxidant and antibacterial activities against S. aureus, it can be used as an effective antidote against atopic dermatitis
Abstract
This study investigates the antibacterial and antioxidant activities of the ethanol extract of Acer tegmentosum Maxim (EATM) against Staphylococcus aureus. The total polyphenol and flavonoid content, 1,1-diphenyl-2- picryl hydrazyl (DPPH) and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging effect, and reducing power of the ferric reducing antioxidant power (FRAP) method were measured to verify the antioxidant activity of the EATM. The antibacterial activity against S. aureus using the EATM was verified by the paper disc method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The total polyphenol and flavonoid contents were 266.66 μg GAE/mg and 6.46 μg QE/mg, respectively. The DPPH and ABTS radical scavenging activity showed a concentration-dependent scavenging activity. The RC50 values of the EATM were 21.49 and 12.81 μg/mL, respectively. A FRAP analysis was conducted for evaluating the reducing power of the EATM and an efficacy of 0.73 ± 0.19 mM FeSO4 E/mg was observed. The antibacterial activity of EATM against S. aureus, determined using the paper disc method, showed an inhibitory ring of 3 mm at 2 mg. The MIC was confirmed at a concentration of ≥ 16 mg/mL, while the MBC was confirmed at 32 mg/mL. As the EATM shows antioxidant and antibacterial activities against S. aureus, it can be used as an effective antidote against atopic dermatitis
참고문헌 (38)
- Adachi Y, "The effect of antibiotics on the production of superantigen from staphylococcus isolated from atopic dermatitis", Journal of Dermatological Science, 28, pp.76~83 (2002)
- Awika JM, "Screening methods to measure antioxidant activity of sorghum(Sorghum bicolor)and sorghum products", Journal of Agricultural and Food Chemistry, 51, pp.6657~6666 (2003)
- Benzie IF, "The ferric reducing ability of plasma(FRAP)as a measure of antioxidant power the FRAP assay", Analytical Biochemistry, 239, pp.70~76 (1996)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Breuer K, "Staphylococcus aureus : colonizing features and influence of an antibacterial treatment in adults with atopic dermatitis", British Journal of Dermatology, 147, pp.55~61 (2002)
- 최준혁, "벌나무 열수 및 에탄올 추출물의 항산화 활성 및 알코올 분해능", 한국식품영양과학회지, 42(3), pp.378~383 (2013), 한국식품영양과학회
- 최종화, "그라비올라잎 열수추출물의 항산화 효능 평가", 한국산학기술학회논문지, 18(6), pp.129~135 (2017), 한국산학기술학회[DOI]
- Duh PD, "Action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage", Food and Chemical Toxicology, 37(11), pp.1055~1061 (1999)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, 12(2), pp.239~243 (1912)
- Hertog MGL, "Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juices", Journal of Agricultural and Food Chemistry, 41, pp.1242~1246 (1993)
- 홍보경, "산겨릅나무 줄기추출물의 생리활성 및 유효성분 분리", 한국약용작물학회지, 15(4), pp.296~303 (2007), 한국약용작물학회
- 허종문, "산겨릅나무 줄기에서 이소프렌계 화합물의 분리", 생약학회지, 38(1), pp.67~70 (2007), 한국생약학회
- 황진우, "Antioxidant and Anti-inflammatory Effects of Hot Water and Ethanol Extracts from Endemic Plants in Indonesia", 대한의생명과학회지, 27(3), pp.161~169 (2021), 대한의생명과학회
- 황윤하, "아토피피부염 소아의 피부집락균 중 Staphylococcus aureus 의 빈도와 항생제 감수성에 관한 연구", Allergy Asthma & Respiratory Diseases, 5(1), pp.21~26 (2017), 대한 소아알레르기 호흡기학회[DOI]
- 황보현, "산수유의 채취 부위에 따른 기능 성분 분석과 항산화 및 항염증 효과에 관한 연구", 생명과학회지, 31(3), pp.287~297 (2021), 한국생명과학회[DOI]
- 장민정, "화장료 소재로서 뜰보리수 과실 추출물의 항산화, 항염증 및 항노화 활성", 대한미용학회지, 17(2), pp.159~167 (2021), 대한미용학회[DOI]
- 강현, "복분자 에탄올 추출물 및 분획물의 항산화 효과", Journal of Plant Biotechnology, 48(4), pp.264~270 (2021), 한국식물생명공학회[DOI]
- 김진영, "Change of Phenylpropanoic Acid and Flavonol Contents at Different Growth Stage of Glasswort (Salicornia herbacea L.)", Food Science and Biotechnology, 23(3), pp.685~691 (2014), 한국식품과학회[DOI]
- 구강모, "옥수수 수염의 항산화 활성과 기능성분 분석", 한국자원식물학회지, 22(4), pp.323~329 (2009), 한국자원식물학회
- 권하나, "산겨릅나무 추출물의 항산화 및 간 기능 보호효과", 한국식품영양과학회지, 37(11), pp.1389~1394 (2008), 한국식품영양과학회
- 권유리, "추출용매에 따른 모링가(Moringa oleifera Lam.) 잎의 항산화 및 생리활성 효과", 한국식품저장유통학회지, 21(6), pp.831~837 (2014), 한국식품저장유통학회
- Lee BH, "A Study of the Effect of Tacrolimus Ointment on the Staphylococcal Colonization in Atopic Dermatitis Patients", Korean Journal of Dermatology, 42(10), pp.1294~1303 (2004)
- Lee GW, "Antioxidant Activity of Leaf Extract from Annona muricata", Review of Korea Contents Association, 14(3), pp.43~46 (2016)
- 이정은, "갓(Brassica juncea L. Czern.)의 잎과 종자 추출물의 항산화, 미백 및 항주름 효능", 아시안뷰티화장품학술지, 18(3), pp.283~295 (2020), 한국피부과학연구원[DOI]
- Lee SH, "Study for Treatment of Atopic Dermatitis in Oriental Medical Prescription", Journal of Korean Medicine, 15, pp.56~69 (2006)
- 이수정, "벌나무(Acer tegmentosum Maxim) 추출물의 항산화 활성 및 고지방식이를 급이한 흰쥐의 지방간 개선 효과", 한국가축위생학회지, 42(2), pp.43~51 (2019), 한국동물위생학회[DOI]
- Leung DYM, "Atopic dermatitis", The Lancet, 361, pp.151~160 (2003)
- Matsui K, "Comparative study of staphylococcus aureus isolated from lesional and non-lesional skin of atopic dermatitis patients", Microbiology and Immunology, 44, pp.945~947 (2000)
- 민택기, "아토피피부염 환자에서 혈청 비타민 D 농도와 메티실린 저항성 황색포도구균 피부 집락화의 연관성", Allergy Asthma & Respiratory Diseases, 1(2), pp.138~143 (2013), 대한 소아알레르기 호흡기학회
- Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", Journal of Ethnopharmacology, 71(1-2), pp.109~114 (2000)
- Ogawa T, "Comparative study of staphylococcal flora on the skin surface of atopic dermatitis patients and healthy subjects", The Journal of Dermatology, 21, pp.453~460 (1994)
- 박성진, "벌나무 잎의 영양성분 및 이화학 특성", Culinary Science & Hospitality Research, 22(8), pp.27~38 (2016), (사)한국조리학회[DOI]
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology & Medicine, 26(9-10), pp.1231~1237 (1999)
- Rice-Evans CA, "The relative antioxidant activities of plant-derived polyphenolic flavonoids", Free radical research, 22(4), pp.375~383 (1995)
- 신인철, "산겨릅나무 추출물의 이화학적 특성과 암세포 성장 억제 효과", Applied Biological Chemistry, 49(4), pp.322~327 (2006), 한국응용생명화학회
- Soares T, "The first serine protease inhibitor from Lasiodora sp", (Araneae : Theraphosidae)hemocytes. Process Biochemistry, 46, pp.2317~2321 (2011)
- Yim YS, "A Study on the Evaluation of the Staphylococcal Exotoxins and Staphylococcal Enterotoxin A-specific IgE Antibody in Childhood Atopic Dermatitis", Korean Journal of Dermatology, 40(6), pp.607~615 (2002)
- Yun HJ, "Clinical Study of Atopic Dermatitis; the Classification of Oriental Medical Clinical type and Treatment", Journal of Korean Oriental Medicine, 22(2), pp.10~21 (2001)