트립신 처리에 따른 적송잎 추출물의 항산화 활성 및 항균 효과
Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract
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Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and ABTS + radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.
Abstract
Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and ABTS + radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.
참고문헌 (24)
- Yang SJ, "Change of Korean Ginseng Components with High Temperature and Pressure Treatment", Korean J Food Sci Technol, 98(4), pp.521~525 (2006)
- 권상철, "황기 효소분해물 열수추출액의 이화학적 특성 및 항산화 활성", 한국식품영양과학회지, 39(3), pp.406~413 (2010), 한국식품영양과학회
- 박민경, "단백질 분해 효소 처리가 청국장의 항산화 활성에 미치는 영향", 한국식품영양과학회지, 40(2), pp.327~333 (2011), 한국식품영양과학회
- 장영선, "포도씨 추출물의 항산화 효과 및 소화효소 저해 효과", 한국식품영양과학회지, 39(6), pp.783~788 (2010), 한국식품영양과학회
- 김선영, "참죽나무 새순 추출물의 항균 활성과 피부 보습효과에 관한 연구", 대한화장품학회지, 36(4), pp.315~321 (2010), 사단법인 대한화장품학회
- 성현철, "사춘기 및 성인 아토피피부염 환자에서 스탬프형 배지를 이용한 황색 포도알균의 정량적인 배양", 대한피부과학회지, 45(7), pp.673~679 (2007), 대한피부과학회
- 양선아, "참소리쟁이 뿌리 추출물 및 분획의 항균 활성과 항생제 증강 활성", 한국약용작물학회지, 25(6), pp.375~380 (2017), 한국약용작물학회[DOI]
- 강성림, "적송잎 추출물의 혈행 및 지질개선 효과", 생명과학회지, 19(4), pp.508~513 (2009), 한국생명과학회
- Kim YS, "Volatile components and antibacterial effects of pine needle (Pinus densiflora S. and Z.) extracts", Food Microbiol, 22, pp.37~45 (2005)[DOI]
- Hsu TY, "Anti-oxidant activity and effect of Pinus morrisonicola Hay. on the survival of leukemia cell line U937", Phytomedicine, 12, pp.663~669 (2005)[DOI]
- Sanders KM, "A Case for Interstitial Cells of Cajal as Pacemakers and Mediators of Neurotransmission in the Gastrointestinal Tract", Gastroenterology, 111, pp.492~515 (1996)[DOI]
- Ward SM, "Mutation of the proto?oncogene c?kit blocks development of interstitial cells and electrical rhythmicity in murine intestine", J Physiol, 480, pp.91~97 (1994)[DOI]
- Tokutomi N, "Rhythmic Cl-current and physiological roles of the intestinal c-kit-positive cells", Pflugers Arch, 431, pp.169~177 (1995)[DOI]
- Thomson L, "Interstitial cells of Cajal generate a rhythmic pacemaker current", Nat Med, 4, pp.848~851 (1998)[DOI]
- Bagchi D, "Protective Effects of Grape Seed Proanthocyanidins and Selected Antioxidants against TPA-Induced Hepatic and Brain Lipid Peroxidation and DNA Fragmentation, and Peritoneal Macrophage Activation in Mice", Gen Pharmacol, 30, pp.771~776 (1998)[DOI]
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Davies R, "The catalysis of the N-nitrosation of secondary amines by nitrosophenols", Food Chem, 6, pp.115~122 (1980)[DOI]
- Blois MS, "Antioxidant Determinations by the Use of a Stable Free Radical", nature, 181, pp.1199~1200 (1958)[DOI]
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)[DOI]
- Lee YJ, "Protective Effects of Ginkgo Biloba Leaf Extract (GBE) against 1,2,4-benzenetriol Induced Toxicity in Vitro", J Environ Health Sci, 27, pp.124~130 (2001)
- 모정희, "화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색", 한국인체미용예술학회지, 14(4), pp.49~60 (2013), 한국인체미용예술학회
- Kang SH, "Identification of Anti-oxidative, Skin Whitening and Anti- wrinkle Constituents from the Leaves of Pinus densiflora and Sargassum Muticum", pp.2015
- 김보영, "의약품 및 개인위생·생활용품 중 보존제 함유량 분석", 한국환경보건학회지, 42(1), pp.53~60 (2016), 한국환경보건학회
- Hwang SH, "Preservation of Scutellariae Radix Extract for Cosmetics", pp.2009