Sophora Flavescens Suppresses Degranulation and Pro-inflammatory Cytokines Production through the Inhibition of NF-κB (p65) Activation in the RBL-2H3 cells
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Sophora flavescens, as a traditional herbal medicine, has been used to treat with a variety of disesases. In previous reports, S. flavescens and sophoraflavanone G (a prenylated flavonoid from S. flavescens) inhibited cytokines productions in LPS-induced Raw 264.7 macrophages cells and BV2 microglial cells. We examined on the anti-allergic effect of S. flavescens on the PMA plus A23187-induced rat leukemia (RBL-2H3) cells. S. flavescens inhibited the release of β-hexosaminidase and productions and expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-4 and cyclooxygenase (COX)-2 in a dose-dependent manner on stimulated RBL-2H3 cells, however, S. flavescens not affect cell viability. The protein expression level of nuclear factor (NF)-κB (p65) was decreased in the nucleus and suppressed the degradation of inhibitory protein IκB-α protein, the activation of extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK) by S. flavescens. These results suggest that S. flavescens could be involved anti-allergic effect by control of NF-κB (p65) translocation into the nucleus through inhibition of IκB-α degradation and suppression of pro-inflammatory cytokines expression.
Abstract
Sophora flavescens, as a traditional herbal medicine, has been used to treat with a variety of disesases. In previous reports, S. flavescens and sophoraflavanone G (a prenylated flavonoid from S. flavescens) inhibited cytokines productions in LPS-induced Raw 264.7 macrophages cells and BV2 microglial cells. We examined on the anti-allergic effect of S. flavescens on the PMA plus A23187-induced rat leukemia (RBL-2H3) cells. S. flavescens inhibited the release of β-hexosaminidase and productions and expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-4 and cyclooxygenase (COX)-2 in a dose-dependent manner on stimulated RBL-2H3 cells, however, S. flavescens not affect cell viability. The protein expression level of nuclear factor (NF)-κB (p65) was decreased in the nucleus and suppressed the degradation of inhibitory protein IκB-α protein, the activation of extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK) by S. flavescens. These results suggest that S. flavescens could be involved anti-allergic effect by control of NF-κB (p65) translocation into the nucleus through inhibition of IκB-α degradation and suppression of pro-inflammatory cytokines expression.
참고문헌 (46)
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