Inhibitory Effect of Rehmannia Glutinosa Pharmacopuncture Solution on β-hexosaminidase Release and Cytokine Production via FcεRI signaling in RBL-2H3 Cells
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Background: Type I allergy is involved in allergic asthma, allergic rhinitis, and atopic dermatitis which are accompanied by an acute and chronic allergic inflammatory responses. Rehmannia glutinosa is a traditional medicine in the East Asian region. This study examined whether a Rehmannia Glutinosa pharmacopuncture solution (RGPS) had anti-allergic or anti-inflammatory effects in antigen-stimulated-RBL-2H3 cells. Methods: We determined the effect of RGPS on cell viability using the 3-[4,5-dimethylthiazolyl]-2,5-diphenyltetrazolium bromide (MTT) assay. We also examined the effect of RGPS on the release of β-hexosaminidase and the secretion of IL-4 and TNF-α using ELISA. In addition, we evaluated the effect of RGPS on the mRNA expression of various cytokines; IL-2, IL-3, IL-4, IL-5, IL-13 and TNF-α using RT-PCR. Furthermore, we assessed the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB using Western blotting after RGPS treatment. Results: We found that RGPS (10-4 to 10-1 dilution) did not cause any cytotoxicity. We observed significant inhibition of β-hexosaminidase release and suppression of the protein secretion of IL-4 and TNF-α and mRNA expression of multiple cytokines in antigen-stimulated-RBL-2H3 cells after RGPS treatment. Additionally, RGPS suppressed not only the phosphorylation of MAPKs, but also the transcriptional activation of NF-κB in antigen-stimulated-RBL-2H3 cells. Conclusions: These results suggest that RGPS inhibits degranulation and expression of cytokines including IL-4 and TNF-α via down-regulation of MAPKs and NF-κB activation in antigen-stimulated-RBL-2H3 cells. In conclusion, RGPS may have beneficial effects in the exerting anti-allergic or anti-inflammatory activities.
Abstract
Background: Type I allergy is involved in allergic asthma, allergic rhinitis, and atopic dermatitis which are accompanied by an acute and chronic allergic inflammatory responses. Rehmannia glutinosa is a traditional medicine in the East Asian region. This study examined whether a Rehmannia Glutinosa pharmacopuncture solution (RGPS) had anti-allergic or anti-inflammatory effects in antigen-stimulated-RBL-2H3 cells. Methods: We determined the effect of RGPS on cell viability using the 3-[4,5-dimethylthiazolyl]-2,5-diphenyltetrazolium bromide (MTT) assay. We also examined the effect of RGPS on the release of β-hexosaminidase and the secretion of IL-4 and TNF-α using ELISA. In addition, we evaluated the effect of RGPS on the mRNA expression of various cytokines; IL-2, IL-3, IL-4, IL-5, IL-13 and TNF-α using RT-PCR. Furthermore, we assessed the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB using Western blotting after RGPS treatment. Results: We found that RGPS (10-4 to 10-1 dilution) did not cause any cytotoxicity. We observed significant inhibition of β-hexosaminidase release and suppression of the protein secretion of IL-4 and TNF-α and mRNA expression of multiple cytokines in antigen-stimulated-RBL-2H3 cells after RGPS treatment. Additionally, RGPS suppressed not only the phosphorylation of MAPKs, but also the transcriptional activation of NF-κB in antigen-stimulated-RBL-2H3 cells. Conclusions: These results suggest that RGPS inhibits degranulation and expression of cytokines including IL-4 and TNF-α via down-regulation of MAPKs and NF-κB activation in antigen-stimulated-RBL-2H3 cells. In conclusion, RGPS may have beneficial effects in the exerting anti-allergic or anti-inflammatory activities.
참고문헌 (24)
- Gilfillan, A.M., "Integrated signalling pathways for mast-cell activation", Nat Rev Immunol, 6(3), pp.218~230 (2006)
- Zhang, R.X., "Rehmannia glutinosa: review of botany, chemistry and pharmacology", J Ethnopharmacol, 117(2), pp.199~214 (2008)
- Shin, M.S., "The effects of Rehmanniae Radix on the propulsive peristalsis of the rat's intestine", J Korean Herbol, 16, pp.189~199 (2001)
- Ko, B.E., "Shimmidojeoksan: treating the Heart for apthae", pp.7~ (2003), All That Korean Medicine
- Ha, C.J., "The prevention of alloxan-induced diabetes in mouse and rabbit by Rehmannial Rhizoma water extract", Med Res, pp.135~142 (1979)
- Cao, Z.L., "Effects of radix Rehmanniae and processed radix Rehmanniae on some immune functions in mice", Zhong Yao Tong Bao, 13(10), pp.22~24 (1988)
- Huang, K.C., "The Pharmacology of Chinese Herbs", pp.280~281 (1993), CRC Press, Inc
- Kim, H., "Effect of Rehmannia glutinosa on immediate type allergic reaction", Int J Immunopharmacol, 20(4), pp.231~240 (1998)
- Sung, Y.Y., "Topical application of Rehmannia glutinosa extract inhibits mite allergen-induced atopic dermatitis in NC", Nga mice, pp.134(1), 37~44
- Hansen, M.B., "Reexamination and further development of a precise and rapid dye method for measuring cell growth", cell kill, 1989, pp.119(2), 203~210
- Theoharides, T.C., "The critical role of mast cells in allergy and inflammation", Ann N Y Acad Sci, 1088, pp.78~99 (2006)
- Lorentz, A., "Role of activator protein 1, nuclear factor-kappaB, and nuclear factor of activated T cells in IgE receptor-mediated cytokine expression in mature human mast cells", J Allergy Clin Immunol, 111(5), pp.1062~1068 (2003)
- Frossi, B., "Selective activation of Fyn", PI3K and p38 MAPK regulates IL-4 production in BMMC under nontoxic stress condition, 2007, pp.178(4), 2549~2555
- Gordon, J.R., "Mast cells as a source of multifunctional cytokines", Immunol Today, 11(12), pp.458~464 (1990)
- Higa, S., "Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils", J Allergy Clin Immunol, 111(6), pp.1299~1306 (2003)
- Rivera, J., "Molecular regulation of mast cell activation", J Allergy Clin Immunol, 117(6), pp.1214~1225 (2006)
- Romagnani, S., "The role of lymphocytes in allergic disease", J Allergy Clin Immunol, 105(3), pp.399~408 (2000)
- Koranteng, R.D., "Differential regulation of mast cell cytokines by both dexamethasone and the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580", Clin Exp Immunol, 137(1), pp.81~87 (2004)
- Tanifuji, S., "Licochalcones suppress degranulation by decreasing the intracellular Ca2+ level and tyrosine phosphorylation of ERK in RBL-2H3 cells", Int Immunopharmacol, 10(7), pp.769~776
- Walczak-Drzewiecka, A., "c-Jun N-terminal kinase is involved in mercuric ions-mediated interleukin-4 secretion in mast cells", Int Arch Allergy Immunol, 136(2), pp.181~190 (2005)
- Wong, C.K., "Molecular mechanisms for the release of chemokines from human leukemic mast cell line (HMC)-1 cells activated by SCF and TNF-alpha: roles of ERK, p38 MAPK, and NF-kappaB", Allergy, 61(3), pp.289~297 (2006)
- Jeong, H.J., "Inhibition of TNF-alpha and IL-6 production by Aucubin through blockade of NF-kappaB activation RBL-2H3 mast cells", Cytokine, 18(5), pp.252~259 (2002)
- Ghosh, S., "NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses", Annu Rev Immunol, 16, pp.225~260 (1998)
- Whiteside, S.T., "I kappa B proteins: structure, function and regulation", Semin Cancer Biol, 8(2), pp.75~82 (1997)