신유․지실 녹용약침이 뇌혈류역학에 미치는 영향
Effects of Phamacopuncture Therapy Using Cervi Pantotrichum Cornu at BL23․BL52 on the Cerebral Hemodynamics in Rats
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The present study was designed to investigate the effects of Phamacopuncture therapy using Cervi Pantotrichum Cornu (PC) at BL23․BL52 on the regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, then the related mechanisms were also investigated. In addition, the present author also investigated the effects of phamacopuncture therapy at BL23․BL52 on the rCBF in cerebral ischemic rats. The results in normal rats were as follows ; PC (3 ㎎/㎏ and 5 ㎎/㎏) at BL23․BL52 significantly increased rCBF but decreased MABP. This result suggests that PC at BL23․BL52 significantly increased rCBF by dilating pial arterial diameter. Increase of PC (5 ㎎/㎏)-induced rCBF was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase and methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. Decrease of PC (5 ㎎/㎏)-induced MABP was significantly increased by pretreatment with methylene blue but was decreased by pretreatment with indomethacin. These results suggested that the action of PC (5 ㎎/㎏) was mediated by guanylate cyclase. The results in cerebral ischemic rats were as follows ; The rCBF was significantly and stably increased by PC (5 ㎎/㎏) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In conclusion, these results suggest that phamacopuncture therapy using Carthami flos at BL23․BL52 can increase rCBF in normal state, and improve stability of rCBF in ischemic state. In addition, the present author also suggest that related mechanisms are involved in guanylate cyclase pathway.
Abstract
The present study was designed to investigate the effects of Phamacopuncture therapy using Cervi Pantotrichum Cornu (PC) at BL23․BL52 on the regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, then the related mechanisms were also investigated. In addition, the present author also investigated the effects of phamacopuncture therapy at BL23․BL52 on the rCBF in cerebral ischemic rats. The results in normal rats were as follows ; PC (3 ㎎/㎏ and 5 ㎎/㎏) at BL23․BL52 significantly increased rCBF but decreased MABP. This result suggests that PC at BL23․BL52 significantly increased rCBF by dilating pial arterial diameter. Increase of PC (5 ㎎/㎏)-induced rCBF was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase and methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. Decrease of PC (5 ㎎/㎏)-induced MABP was significantly increased by pretreatment with methylene blue but was decreased by pretreatment with indomethacin. These results suggested that the action of PC (5 ㎎/㎏) was mediated by guanylate cyclase. The results in cerebral ischemic rats were as follows ; The rCBF was significantly and stably increased by PC (5 ㎎/㎏) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In conclusion, these results suggest that phamacopuncture therapy using Carthami flos at BL23․BL52 can increase rCBF in normal state, and improve stability of rCBF in ischemic state. In addition, the present author also suggest that related mechanisms are involved in guanylate cyclase pathway.
참고문헌 (38)
- 全國韓醫科大學 本草學敎授, "本草學", pp.545~546 (1999), 永林社
- 김진규, "Inhibitory effects of Cervi Pantotrichum Cornu herbal acupuncture on type II collagen-induced arthritis", 대한침구학회지, 19(6), pp.155~170 (2002), 대한침구학회
- 全國韓醫科大學 鍼灸經穴學敎室, "鍼灸學(上)", pp.489~490 (1991), 集文堂
- 의과대학 신경해부학교수, "증례를 통한 임상신경해부", pp.191~ (1998), 한우리
- Kety, S.S, "The nitrous oxide method for the man ; theory, procedure and normal values", J. Clin Invest, 27, pp.476~483 (1948)
- 대한신경외과학회, "신경외과학", pp.276~279 (1997), 중앙문화사
- 이경은, "허혈, 재관류 손상에서 뇌조직 아민 변동과 Free Radical과의 관련성", 대한신경과학회지, 8(1), pp.2~8 (1990)
- 통계청, "사망원인 통계 연보" (2008), 통계청
- Glanz, M, "Functional electrostimulation in poststroke rehabilitation ; a meta-analysis of the randomized controlled trials", Arch. Phys. Med. Rehabil, 77(6), pp.549~553 (1996)
- 정현우, "석창포가 혈압 및 국소 뇌혈류량에 미치는 영향", 대한본초학회지, 14(2), pp.81~88 (1999)
- 김남순, "소회향이 흰쥐의 국소뇌혈류량 및 평균혈압에 미치는 영향", 동의생리병리학회지, 21(3), pp.652~657 (2007), 대한동의병리학회
- 최재영, "흰쥐의 중뇌동맥 폐쇄후 當歸芍藥散 투여가 여성호르몬 및 뇌위축에 미치는 영향", 한방성인병학회지, 4(1), pp.70~154 (1998)
- 유영대, "전침자극과 운동치료가 허혈성 뇌졸중 백서모델의 신경계에 미치는 영향", 동의생리병리학회지, 20(4), pp.1014~1020 (2006)
- 김재홍, "腎兪, 太谿의 冬蟲夏草 藥鍼과 經口 投與가 좌측 腎臟 제거 白鼠에 대한 腎臟 Aquaporin-2 蛋白 發現과 腎臟機能에 미치는 影響", 대한침구학회지, 22(1), pp.61~76 (2005), 대한침구학회
- 최원확, "加味獨活寄生湯의 經口投與와 志室·懸鍾 電鍼 倂用 施術이 흰 쥐의 骨多孔症에 미치는 影響", 대한침구학회지, 23(3), pp.1~19 (2006), 대한침구학회
- 이준무, "腎兪, 志室의 八味地黃湯藥鍼處理가 토끼, 기니어 픽 및 Rat의 血液象에 미치는 영향", 大韓藥鍼學會誌, 2(1), pp.63~71 (1999)
- 이현진, "녹용약침이 백서의 제2형 Collagen 유발 관절염에서 iNOS 발현과 NO 생성 억제에 미치는 영향", 대한침구학회지, 25(5), pp.105~116 (2008), 대한침구학회
- 김영태, "鹿茸 및 鹿茸藥鍼이 동물의 成長과 知能發達에 미치는 影響", 대한침구학회지, 18(5), pp.122~134 (2001)
- 姜起遠, "鹿茸四斤丸藥鍼 및 經口投與가 흰쥐의 成長과 知能發達에 미치는 影響", 대한침구학회지, 20(6), pp.7~ (2003), 대한침구학회
- 김성옥, "鹿茸藥鍼이 국소 뇌허혈 모델 백서에서 방사형 미로과제학습 및 뇌신경보호에 미치는 영향", pp.2005
- 변부형, "유전독성시험에 의한 녹용약침의 안전성 연구", 대한본초학회지, 20(2), pp.1~6 (2005), 대한본초학회
- Iwamoto, J, "Methylene blue inhibits hypoxic cerebral vasodilation in awake sheep", J. Appl Physiol, 73(6), pp.2226~2232 (1992)
- Wang, Q, "Comparison of the effects of NG-nitro-L-arginine and indomethacin on the hypercapnic cerebral blood flow increase in rats", Brain Res., 641(2), pp.257~264 (1994)
- Choi, D.W, "Glutamate neurotoxicity and disease of the nervous system", J. Neuron., 1, pp.623~634 (1988)
- Cain, B.S, "Tumor necrosis factor-alpha and interleukin-1beta synergistically depress human myocardial function", Crit Care Med, 27(7), pp.1309~1318 (1999)
- Hayashi, Y, "Immunohistochemical investigation of caspase-1 and effect of caspase-1 inhibitor in delayed neuronal death after transient cerebral ischemia", Brain Res, 893, pp.113~120 (2001)
- Chen, S.T, "A model of focal ischemic stoke in the rat. reproducible extension cortical infarction", Stroke, 17, pp.738~743 (1986)
- Longa, E.Z, "Reversible middle cerebral artery occlusion without craniectomy in rats", Stroke, 20(1), pp.84~91 (1989)
- 이혜정, "약침학 개론 및 임상", pp.13~15 (1999), 일중사
- 한상원, "鹿茸藥鍼이 骨形成에 미치는 영향", 대한침구학회지, 18(5), pp.135~146 (2001)
- 김우겸, "인체의 생리", pp.30~47 (1985), 서울대학교 출판부
- Guyton, "의학생리학(제10판)", pp.170~175 (2002), 정담사
- 김경환, "이우주의 약리학 강의(제4판)", pp.82~120 (1998), 의학문화사
- 성호경, "생리학(제6판)", pp.110~ (1996), 의학문화사
- Palmer, P.M.J, "Nitric oxide release accounts for the biology activity of endothelium derived relaxing factor", Nature, 327, pp.524~526 (1990)
- Shibuki, K, "Endogenous nitric oxide release required for long term synaptic depression in the cerebellum", Nature, 349, pp.326~328 (1991)
- 곽륭찬, "도해뇌신경외과학", pp.343~351 (1992), 제일의학사
- McCord, J.M, "Mechanisms of disease ; oxygen-derived free radicals in postischemic tissue injury", New Eng J. Med., 312, pp.159~163 (1985)