天麥湯의 알레르기 염증반응 및 기전탐색에 관한 연구
Experimental Study about the Pathway of Inflammatory Allergic Reaction of Cheonmaec-tang
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Objetives The purpose of this study was to examine the pathway of anti-allergic effects of Cheonmaec-tang (CMT). Methods We examined the cell viability, β-hexosaminidase release, pro-inflammatory cytokines secretion and mRNA expressions, nuclear factor-kappa B (NF-κB) (p65) activation, inbibitor kappa B-alpha (IκB-α) degradation, and MAPKs activation in RBL-2H3 cells pre-treated by CMT of 2.0 ㎎/㎖, 4.0 ㎎/㎖ separately. Results We observed that CMT reduced the secretion of β-hexosaminidase, TNF-α, IL-4 and the expression of COX-2 mRNA in RBL-2H3 cells. Futhermore, CMT inhibited the levels of activation of NF-κB (p65) protein, ERK MAPK, and degradation of IκB-α in RBL-2H3 cells. Conclusoins These results show that CMT has an anti-histamine effect and inhibitory effect of NF-κB (p65) through regulation of IκB-α degradation. These suggest that CMT could be used as an anti-allergic medicine.
Abstract
Objetives The purpose of this study was to examine the pathway of anti-allergic effects of Cheonmaec-tang (CMT). Methods We examined the cell viability, β-hexosaminidase release, pro-inflammatory cytokines secretion and mRNA expressions, nuclear factor-kappa B (NF-κB) (p65) activation, inbibitor kappa B-alpha (IκB-α) degradation, and MAPKs activation in RBL-2H3 cells pre-treated by CMT of 2.0 ㎎/㎖, 4.0 ㎎/㎖ separately. Results We observed that CMT reduced the secretion of β-hexosaminidase, TNF-α, IL-4 and the expression of COX-2 mRNA in RBL-2H3 cells. Futhermore, CMT inhibited the levels of activation of NF-κB (p65) protein, ERK MAPK, and degradation of IκB-α in RBL-2H3 cells. Conclusoins These results show that CMT has an anti-histamine effect and inhibitory effect of NF-κB (p65) through regulation of IκB-α degradation. These suggest that CMT could be used as an anti-allergic medicine.
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