Helicobacter pyJori의 신속한 진단을 위한 연구

Abstract

Optimal conditions for the rapid diagnosis of Helicobacter pνlori were determined. Bacterial strains were isolated from biopsy where the u1cer was observed with endoscope. Morphological and cultural properties of the isolates were determined. Efficiency and srnsitivity of several diagnostic tests were compared. ulcer tissues as well as pure cultures of H. pylori showed positive reaction within 5 min in urease test broth or semi-solid agar containing 10% urea and 0.1% phenol red. Urease test was highly reproducible and sensitive. Urease A gene extracted from pure culture and tissue was amplified by nested PCR using HEPY 1⁄2 and HEPY3⁄4 primers. Electrophoresis of PCR product confirmed that DNAs extracted from tissues and isolates which show positive reaction in urease test contain Urease A gene. Among several diagnostic methods used in general, urease test was recommended as a convenient, cheap and easy method.