Random Amplified Polymorphic DNA 분석에 의한 Helicobacter pylori의 분자적 형별 분석

Abstract

This study was undertaken to detennine molecular types and genetic diversity of Helicobacter pylori isolate obtained from various gastroduodenal diseases. Antral biopsies were taken for culture from 90 patients with chronic gastritis gastric ulcer and duodenal ulcer at the time of endoscopy. H. pylori was identified by typical gram stain morphological and biochemical tests. UreA of the candidates were Amplified using PCR, and 30 strainswere identified as H. pylori from 90 specimens. RAPD fmgerprinting was performed by 3 primers (OPA-11, 5’-CAATCGCCGT -3’; OPA-18, 5’-AGGTGACCGT -3’ OPA-19, 5’-CAAACGTCGG-3’Operon Technologies, Atlanta, GA). Relationship of the strains shown by DNA band pattem was analyzed numerically and the result were expressed as phensgram. All isolates were divided into five molecular types (Ⅰ-V) at similarity (S) value of 0.4; 11 strains (36.6%), 6 strains (20.0%), 5 strains (16.7%), 5 strains (16.7%) and 3 strains (10.0%) belonged to type Ⅱ, Ⅰ, Ⅲ V and Ⅳ, respectively. The mean S values of all isolates, type Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were 0.62, 0.60, 0.66, 0.58 and 0.55, respectively. H. pylori isolates had high level of genetic diversity. The RAPD molecular types of H. pylori were not disease-specific since the types were diverse in the is이ates from various gastroduodenal diseases.