건조방법에 따른 미선나무 잎의 항산화 및 항염증 효과
Antioxidant activities and anti-inflammatory effects of fresh and air-dried Abeliophyllum distichum Nakai leaves
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In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.
Abstract
In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.
참고문헌 (32)
- Maxwell SR, "Prospects for the use of antioxidant therapies", Drugs, 49, pp.345~361 (1995)
- Asensi M, "Natural polyphenols in cancer therapy", Crit Rev Clin Lab Sci, 48, pp.197~216 (2011)
- Kehrer JP, "Free radicals as mediators of tissue injury and disease", Crit Rev Toxicol, 23, pp.21~48 (1993)
- Lawrence T, "Anti-inflammatory lipid mediators and insights into the resolution of inflammation", Nat Rev Immunol, 2, pp.787~795 (2002)
- Choi DS, "Study on the antiinflammatory effect and mechanism of isoegomaketone in vitro and in vivo", pp.2009
- Chae CH, "Functional role of inducible nitric oxide synthase and cyclooxygenase-2 in acute inflammation", Ministry of Health and Welfare, 10~11 (2004)
- 박종필, "Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts", 한국약용작물학회지, 19(5), pp.373~379 (2011), 한국약용작물학회
- 정호경, "LPS로 유도한 RAW 264.7 세포의 염증반응에서 뚝새풀 에탄올추출물의 억제 효과", 한국약용작물학회지, 22(2), pp.98~104 (2014), 한국약용작물학회[DOI]
- 박재호, "미선나무 잎 추출물의 항산화 및 산화적 DNA 손상억제 활성", 대한본초학회지, 26(4), pp.95~99 (2011), 대한본초학회
- Oh H, "Four glycosides from the leaves of Abeliophyllum distichum with inhibitory effects on angiotensin converting enzyme", Phytother Res, 17, pp.811~813 (2003)
- 박광훈, "Anti-Inflammatory Effect of the Extracts from Abeliophyllum distichum Nakai in LPS-Stimulated RAW264.7 Cells", 한국자원식물학회지, 27(3), pp.209~214 (2014), 한국자원식물학회[DOI]
- Xiong Q, "Hepatoprotective activity of phenylethanoids from Cistanche deserticola", Planta Med, 64, pp.120~125 (1998)
- Schlesier K, "Assessment of antioxidant activity by using different in vitro methods", Free Radic Res, 36, pp.177~187 (2002)
- He ZD, "Antioxidant activity of phenylethanoid glycosides from Brandisia hancei", J Ethnopharmacol, 71, pp.483~486 (2000)
- Li J, "Protection of phenylpropanoid glycosides from pedicularis against oxidative hemolysis in vitro", Planta Med, 59, pp.315~317 (1993)
- Xiong Q, "Antioxidative effects of phenylethanoids from Cistanche deserticola", Biol Pharm Bull, 19, pp.1580~1585 (1996)
- Bondet V, "Kinetics and mechanisms of antioxidant activity using the DPPH free radical method", LWT-Food Sci Technol, 30, pp.609~615 (1997)
- Van den Berg R, "Applicability of an improved trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, 66, pp.511~517 (1999)
- Oyaizu M, "Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)
- AOAC, "Official Methods of Analysis", pp.8~35 (1995), Association of Official Analytical Chemists
- Que F, "Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds", Food Res Int, 39, pp.581~587 (2006)
- Ferreira IC, "Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity", Food Chem, 100, pp.1511~1516 (2007)
- Ferreres F, "Improved loquat (Eriobotrya japonica Lindl.) cultivars: Variation of phenolics and antioxidative potential", Food Chem, 114, pp.1109~1127 (2009)
- Bremer B, "Phylogenetics of asterids based on 3 coding and 3 non-coding chloroplast DNA markers and the utility of non-coding DNA at higher taxonomic levels", Mol Phylogenet Evol, 24, pp.274~301 (2002)
- Szabo C, "Role of nitric oxide in endotoxic shock: An overview of recent advances", Ann N Y Acad Sci, 851, pp.422~425 (1998)
- Martel-Pelletier J, "Cyclooxygenase-2 and prostaglandins in articular tissues", Semin Arthritis Rheum, 33, pp.155~167 (2003)
- Weisz A, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferongamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine", Biochem J, 316, pp.209~215 (1996)
- 안상미, "국산 생약 추출물의 항염증 활성 스크리닝", 대한화장품학회지, 40(1), pp.95~108 (2014), 사단법인 대한화장품학회[DOI]
- Kang CH, "Inhibition of lipopolysaccharide-induced iNOS, COX-2, and TNF-<TEX>$\alpha$<", TEX> expression by aqueous extract of Orixa japonica in Raw 264.7 cells via suppression of NF-<TEX>${\kappa}B$<, Trop J Pharm Res, pp.2011, 10[DOI]
- Hur GM, "Hepatic ischemia", reperfusion in rats induces iNOS gene transcription by activation of NF-kappaB, 1999, pp.261, 917~922
- Sato T, "Enhancement of prostaglandin E2 production by epidermal growth factor requires the coordinate activation of cytosolic phospholipase A2 and cyclooxygenase 2 in human squamous carcinoma A431 cells", Prostaglandins, 53, pp.355~369 (1997)
- Huang M, "Non-small cell lung cancer cyclooxygenase-2-dependent regulation of cytokine balance in lymphocytes and macrophages: up-regulation of interleukin 10 and down-regulation of interleukin 12 production", Cancer Res, 58, pp.1208~1216 (1998)