Comparison of Anti-oxidant and Anti-inflammatory Activities of Methanolic Extracts Obtained from Different Parts of Cotoneaster wilsonii Nakai
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Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity (IC50; 15.74 ㎍/㎖), and it also had the highest total phenolic and flavonoid content (220.95 ㎎·GAE/g, and 36.46 ㎎·QE/g extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 ㎎/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.
Abstract
Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity (IC50; 15.74 ㎍/㎖), and it also had the highest total phenolic and flavonoid content (220.95 ㎎·GAE/g, and 36.46 ㎎·QE/g extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 ㎎/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.
참고문헌 (24)
- Bauer G., "Reactive oxygen and nitrogen species: Efficient, selective, and interactive signals during intercellular induction of apoptosis", Anticancer Research, 20, pp.4115~4139 (2000)
- Blois MS., "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Chang CS, "Leaf flavonoids in Cotoneaster wilsonii(Rosaceae) from the island Ulleung-do, Korea", Biochemical Systematics and Ecology, 31, pp.171~179 (2003)
- Chung HY, "Molecular inflammation: Underpinnings of aging and age-related diseases", Ageing Research Reviews, 8, pp.18~30 (2009)
- Yishan Duan, "Antioxidant Activities of n-Butanol and Ethyl Acetate Extracts from Yam (Dioscorea batatas DECNE.)", 한국응용과학기술학회지, 32(4), pp.599~606 (2015), 한국응용과학기술학회
- Elizabeth A. Miles, "Differential anti-inflammatory effects of phenolic compounds from extra virgin olive oil identified in human whole blood cultures", Nutrition, 21, pp.389~394 (2005)
- Green DR, "Pharmacological manipulation of cell death: Clinical applications in sight?", Journal of clinical investigation, 115, pp.2610~2617 (2005)[DOI]
- Hwang SJ, "Anti-inflammatory effects of chlorogenic acid in lipopolysaccharidestimulated RAW264.7 cells", Inflammation Research, 63, pp.81~90 (2014)
- 김찬식, "Inhibitory Effects of Chlorogenic Acid on Aldose Reductase Activity In Vitro and Cataractogenesis in Galactose-fed Rats", Archives of Pharmacal Research, 34(5), pp.847~852 (2011), 대한약학회
- 김혜수, "정금나무 열매의 총 페놀 함량 및 항산화활성 분석", 한국산림과학회지, 102(4), pp.566~570 (2013), 한국산림과학회[DOI]
- Kim YH, "The antioxidant activity and their major antioxidant compounds from Acanthopanax senticosus and A. koreanum", Molecules, 20, pp.13281~13295 (2015)
- 김용식, "울릉도 섬개야광나무의 보전학적 고려", 식물분류학회지, 32(2), pp.3~175 (2002), 한국식물분류학회
- Lim JS, "A faunastic study of insects from is. Ulleungdo and its nearby islands in South Korea", Journal of Asia-Pacific Biodiversity, 6, pp.93~121 (2013)
- Liu XP, "Antioxidant activities of extracts from sarcocarp of Cotoneaster multiflorus", Journal of Chemistry, 2018, pp.4619768~ (2018)[DOI]
- Mosmann T., "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, pp.55~63 (1983)
- Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentine", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- Nordberg J, "Reactive oxygen species, antioxidants, and the mammallan thioredoxin system", Free Radical Biology and Medicine, 31, pp.1287~1312 (2001)
- Omaye ST, "Effect of butylated hydroxytoluene and other antioxidants on mouse lung metabolism", Journal of Toxicology and Environmental Health, 3, pp.829~836 (1997)
- Oyaizu M., "Studies on products of browning reactions:Antioxidative activities of products of browning reaction prepared from glucosamine", Japanese Journal of Nutrition, 44, pp.307~315 (1986)
- Rice-Evans CA, "Structureantioxidant activity relationships of flavonoids and phenolic acids", Free Radical Biology and Medicine, 20, pp.933~956 (1996)
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", American Journal of Enology and Viticulture, 16, pp.144~158 (1965)
- Uysal A, "Chemical and biological insights on Cotoneaster integerrimus: A new(-)-epicatechin source for food and medicinal applications", Phytomedicine, 23, pp.979~988 (2016)
- 이미란, "콜라비 추출물의 항산화 및 항염 효능", 한국응용과학기술학회지, 34(2), pp.189~202 (2017), 한국응용과학기술학회
- Zou Y, "Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in southern China", International Journal of Molecular Sciences, 13, pp.16544~16553 (2012)[DOI]