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Comparison of γ-aminobutyric acid and isoflavone aglycone contents, to radical scavenging activities of high-protein soybean sprouting by lactic acid fermentation with Lactobacillus brevis

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주제분류식품과학

초록

In this study, soy-powder yogurt (SPY) with enhanced levels of γ-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and 491.25 µg/g in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

Abstract

In this study, soy-powder yogurt (SPY) with enhanced levels of γ-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and 491.25 µg/g in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

참고문헌 (45)

  1. 조대윤, "Analysis of the isoflavone content, antioxidant activity, and SDS-PAGE of cheese analogs produced with different proteolysis and soymilk residue contents", Applied Biological Chemistry, 58(4), pp.501~509 (2015), 한국응용생명화학회
  2. 구성철, "Biochemical and proteomic analysis of soybean sprouts at different germination temperatures", Applied Biological Chemistry, 58(3), pp.397~407 (2015), 한국응용생명화학회
  3. 이진환, "Changes in Phenolic Compounds (Isoflavones and Phenolic acids) and Antioxidant Properties in High-Protein Soybean (Glycine max L., cv. Saedanbaek)for Different Roasting Conditions", Applied Biological Chemistry, 56(5), pp.605~612 (2013), 한국응용생명화학회
  4. Huang X, "Kinetic changes of nutrients and antioxidant capacities of germinated soybean (Glycine max. L) and mung bean (Vigna radiata L.) with germination time", Food Chem, 143, pp.268~276 (2014)
  5. Matsuyama A, "Characterization of glutamate decarboxylase mediating <TEX>$\gamma$<", TEX>-amino butyric acid increase in the early germination stage of soybean (Glycine max L. Merr), 2009, pp.107, 538~543
  6. Wang F, "Isoflavone, <TEX>$\gamma$<", TEX>-aminobutyric acid contents and antioxidant activities are significantly increased during germination of three Chinese soybean cultivars, 2015, pp.14, 596~604
  7. Xu JG, "Changes in <TEX>$\gamma$<", TEX>-aminobutyric acid content and related enzyme activities in Jindou 25 soybean (Glycine max L.) seeds during germination, 2014, pp.55, 341~346
  8. Paucar-Menacho LM, "Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivars BRS 258", Food Res Int, 43, pp.1856~1865 (2010)
  9. Cevallos-Casals BA, "Impact of germination on phenolic content and antioxidant activity of 13 edible seed species", Food Chem, 119, pp.1485~1490 (2010)
  10. Park KB, "Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract", Bioresour Technol, 98, pp.1675~1679 (2007)
  11. Chen C, "Study on the conditions to brew rice vinegar with high content of <TEX>$\gamma$<", TEX>-amino butyric acid by response surface methology, 2009, pp.87, 334~340
  12. Thuwapanichayanan R, "Enhancement of <TEX>$\gamma$<", TEX>-aminobutyric acid in germinated paddy by soaking in combination with anaerobic and fluidized bed heat treatment, 2015, pp.95, 55~62
  13. Narayan VS, "Metabolism, enzymology and possible role of 4-aminobutyrate in higher plants", Phytochemistry, 29, pp.367~375 (1990)
  14. Guo Y, "Effects of soaking and aeration treatment on <TEX>$\gamma$<", TEX>-aminobutyric acid accumulation in germinated soybean (Glycine max L.), 2011, pp.232, 787~795
  15. Komatsuzaki N, "Production of <TEX>$\gamma$<", TEX>-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods, 2005, pp.22, 497~504
  16. Komatsuzaki N, "Effect of soaking and gaseous treatment on GABA content in germinated brown rice", J Food Eng, 78, pp.556~560 (2007)
  17. Wang HF, "Comparison of bioactive components in GABA tea and green tea produced in Taiwan", Food Chem, 96, pp.648~653 (2006)
  18. Diana M, "Free amino acid profile of Spanish artisanal cheeses: Importance of gamma-aminobutyric acid (GABA) and ornithine content", J Food Compos Anal, 35, pp.94~100 (2014)
  19. Park KB, "Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium Lactobacillus brevis OPK-3", Bioresour Technol, 98, pp.312~319 (2007)
  20. Yokoyama S, "Production of <TEX>$\gamma$<", TEX>-aminobutyric acid from alcohol distillery lees by Lactobacillus brevis IFO-12005, 2002, pp.93, 95~97
  21. Jeng KC, "Effect of microbial fermentation on content of strain, GABA, and polyphenolics in Pu-Erh tea", J Agric Food Chem, 55, pp.8787~8792 (2007)
  22. Lin SD, "Bioactive components and antioxidant properties of <TEX>$\gamma$<", TEX>-aminobutyric acid (GABA) tea leaves, 2012, pp.46, 64~70
  23. Komatsuzaki N, "Effect of soaking and gaseous treatment on GABA content in germinated brown rice", J Food Eng, 78, pp.556~560 (2007)
  24. 김남연, "Characterization of Soybean Fermented by Aflatoxin Non-producing Aspergillus oryzae and γ-Aminobutyric Acid Producing Lactobacillus brevis", Applied Biological Chemistry, 57(6), pp.703~708 (2014), 한국응용생명화학회
  25. 황정은, "잠재적인 생균제제 Lactobacillus plantarum P1201을 이용한 항산화 활성이 증진된 두유-요구르트 제조", 한국식품과학회지, 46(5), pp.556~565 (2014), 한국식품과학회[DOI]
  26. Kumar V, "Changes in lipoxygenase isozymes and trypsin inhibitor activity in soybean during germination at different temperatures", Food Chem, 99, pp.563~568 (2006)
  27. Shi H, "Comprehensive profiling of isoflavones, phytosterols, tocopherols, minerals, crude protein, lipid, and sugar during soybean (Glycine max) germination", J Agric Food Chem, 58, pp.4970~4976 (2010)
  28. Kurata K, "Orally administered L-ornithine elevates brain L-ornithine levels and has an anxiolytic-like effect in mice", Nutr Neurosci, 14, pp.243~248 (2011)
  29. Sugino T, "L-ornithine supplementation attenuates physical fatigue in healthy volunteers by modulating lipid and amino acid metabolism", Nutr Res, 28, pp.738~743 (2008)
  30. Pinho O, "Effect of temperature on evolution of free amino acid and biogenic amine contents during storage of azeitao cheese", Food Chem, 75, pp.287~291 (2001)
  31. Ko CY, "Gamma-aminobutyric acid production in black soybean milk by Lactobacillus brevis FPA 3709 and the antidepressant effect of the fermented product on a forced swimming rat model", Process Biochem, 48, pp.559~568 (2013)
  32. Chung HJ, "Effects of steeping and anaerobic treatment on GABA (<TEX>$\gamma$<", TEX>-amino butyric acid) content in germinated waxy hull-less barley, 2009, pp.42, 1712~1716
  33. Bouche N, "GABA in plants: just a metabolite?", Trends Plant Sci, 9, pp.111~115 (2004)
  34. Streeter JG, "Anaerobic accumulation of <TEX>$\gamma$<", TEX>-aminobutyric acid and alanine in radish leaves (Raphanus sativus L.), 1972, pp.49, 572~578
  35. Bai Q, "Effects of components in culture medium on glutamate decarboxylase activity and <TEX>$\gamma$<", TEX>-aminobutyric acid accumulation in foxtail millet (Seratia italica L.) during germination, 2009, pp.116, 152~157
  36. Makno Y, "Stimulation of <TEX>$\gamma$<", TEX>-amino butyric acid production in vine-ripe tomato (Lycopersicon esculentum Mill.) fruits under modified atmospheres, 2008, pp.56, 7189~7193
  37. Lin PY, "Bioactive compounds in legumes and their germinated products", J Agric Food Chem, 54, pp.3807~3814 (2006)
  38. Yu O, "Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues", J Plant Physiol, 124, pp.781~793 (2000)
  39. Hahlbrock K, "Physiology and molecular biology of phenylpropanoid metabolism", Annu Rev Plant Physicol, 40, pp.347~369 (1989)
  40. Devi MKA, "Functional attributes of soybean seeds and products, with reference to isoflavone content and antioxidant activity", Food Chem, 114, pp.771~776 (2009)
  41. Rice-Evans C, "Antioxidant properties of phenolic compounds", Trends Plant Sci, 2, pp.152~159 (1997)
  42. Chien HL, "Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria", Food Microbiol, 23, pp.772~778 (2006)
  43. Chung IM, "Effect of processing, fermentation, and aging treatment to content and profile of phenolic compounds in soybean seed, soy curd and soy paste", Food Chem, 127, pp.960~967 (2011)
  44. Youn KS, "Optimization of the roasting temperature and time for preparation of coffee-like maize beverage using the response surface methodology", LWT-Food Sci Technol, 46, pp.305~310 (2012)
  45. Juan MY, "Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715", Food Microbiol, 27, pp.589~591 (2010)

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