단일클론항체를 사용한 타액 내 Streptococcus mutans 검출
Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구의 목적은 Streptococcus mutans의 Ag I/II와 glucosyltransferase에 대해 개발된 단일클론항체를 사용한 타액내 Streptococcus mutans 검출의 효용성을 평가하기 위함이다. 세 군(성인, 미성년자, 그리고 교정치료중인 미성년자)을대상으로 DMFT, Dentocult-SM strip mutans를 이용한 타액내의 colony density (CFU/mL), polymerase chain reaction을이용한 Sreptococcus mutans와 Sreptococcus sobrinus의 존재여부, 그리고 4가지 단일클론항체를 사용한 타액내 Streptococcus mutans 검출 실험을 시행하였다. 그 결과 Streptococcus mutans가 Streptococcus sobrinus보다 치아우식 형성에 더 주요하게 작용한다는 것을 입증하였으며, glucosyltransferases (gtf B, gtf C, gtf D)와 Ag I/II에 대한 단일클론항체들의 Streptococcus mutans를 검출해 내는 능력이 Dentocult-SM strip mutans나 polymerase chain reaction을사용한 검출법보다 뛰어남을 보였다.
Abstract
The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans insaliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) inStreptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), theresults of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using Dentocult-SMstrip mutans, polymerase chain reaction was performed to test whether Streptococcus mutans and Streptococcussobrinus were present, and Streptococcus mutans detecting tests performed in their saliva using four types ofmonoclonal antibody were collected. In conclusion, it was demonstrated that the Streptococcus mutants plays more important role in forming dentalcaries compared to Streptococcus sobrinus, and that the monoclonal antibodies against glucosyltransferases(gtf B, gtf C, gtf D) and Ag I/II of Streptococcus mutans are superior in detecting Streptococcus mutans toDentocult-SM strip mutans or polymerase chain reaction.
참고문헌 (31)
- Petersen PE, "Challenges to improvement of oral health in the 21st century the approach of the WHO Global Oral Health Programme", Int Dent J, 54, pp.329~343 (2004)
- "Korea National Oral Health Survey" (2012)[URL]
- Keyes PH, "Mechanisms of Hard Tissue Destruction", pp.261~283 (1963), Academy Press
- Selwitz RH, "Dental caries", Lancet, 369, pp.51~59 (2007)
- Marsh PD, "Microbiological aspects of the chemical control of plaque and gingivitis", J Dent Res, 71, pp.1431~1438 (1992)
- Ajdic′ D, "Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen", Proc Natl Acad Sci USA, 99, pp.14434~14439 (2002)
- Jenkinson HF, "Structure, function and immunogenicity of streptococcal antigen I", II polypeptides, 1997, pp.23, 183~190
- Taubman MA, "The scientific and publichealth imperative for a vaccine against dental caries", Nat Rev Immunol, 6, pp.555~563 (2006)
- "Rationale and Evidence for the International Caries Detection and Assessment System (ICDAS II)"[URL]
- Köhler B, "The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age", Oral Microbiol Immunol, 3, pp.14~17 (1988)
- Okada M, "PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children", J Med Microbiol, 51, pp.443~447 (2002)
- Okada M, "Longitudinal studies on dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-schoolchildren", J Med Microbiol, 54, pp.661~665 (2005)
- Jenkinson HF, "Structure, function and immunogenicity of streptococcal antigen I", II polypeptides, 1997, pp.23, 183~190
- Nakano K, "Contribution of cell-surface protein antigen PAc of Streptococcus mutans to bacteremia", Microbes Infect, 8, pp.114~121 (2006)
- Brandtzaeg P, "Overview of the mucosal immune system", Curr Top Microbiol Immunol, 146, pp.13~25 (1989)
- Rozen R, "The role of fructans on dental biofilm formation by Streptococcus sobrinus, Streptococcus mutans, Streptococcus gordonii and Actinomyces viscosus", FEMS Microbial Lett, 195, pp.205~210 (2001)
- Carlsson J, "Microbial aspects of frequent intake of products with high sugar concentrations", Scand J Dent Res, 97, pp.110~114 (1989)
- Russell MW, "Secretory immunity in defense against cariogenic mutans streptococci", Caries Res, 33, pp.4~15 (1999)
- Taubman MA, "Significance of salivary antibody in dental disease", Ann N Y Acad Sci, 694, pp.202~215 (1993)
- Gregory RL, "Function of anti-streptococcus mutans antibodies :anti-ribodomal antibodies inhibit acid production, growth, and glucose phosphotransferase activity", Infect Immun, 45, pp.286~289 (1984)
- Childers NK, "A controlled clinical study of the effect of nasal immunization with a Streptococcus mutans antigen alone or incorporated into liposomes on induction of immune responses", Infect Immun, 67, pp.618~623 (1999)
- Liu C, "Effects of targeted fusion anti-caries DNA vaccine pGJA-P", VAX in rats with caries, 2008, pp.26, 6685~6689
- Ma JKC, "An investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against Streptococcus mutans", Infect Immun, 58, pp.3407~3414 (1990)
- Shi W, "Rapid and quantitative detection of Streptococcus mutans with speciesspecific monoclonal antibodies", Hybridoma, 17, pp.365~371 (1998)
- Wennerholm K, "Comparison of Saliva-Check Mutans and Saliva-Check IgA Mutans with the Cariogram for caries risk assessment", Eur J Oral Sci, 121, pp.389~393 (2013)
- 김추성, "단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정", 대한소아치과학회지, 37(2), pp.186~192 (2010), 대한소아치과학회
- Ikebe K, "Association of salivary Streptococcus mutans levels determined by rapid detection system using monoclonal antibodies with prevalence of root surface caries", Am J Dent, 21, pp.283~287 (2008)
- 조평규, "경남지역 특수학교 학생의 구강내 세균검사에 따른 영구치우식경험율에 관한 연구", 한국치위생학회지, 8(4), pp.115~125 (2008), 한국치위생학회
- Chia JS, "Antigenicity of a synthetic peptide from glucosyltransferases of Streptococcus mutans in humans", Infect Immun, 65, pp.1126~1130 (1997)
- Nishikawara F, "Correlation of cariogenic bacteria and dental caries in adults", J Oral Sci, 48, pp.245~251 (2006)
- Gu F, "Analyses of Streptococcus mutans in saliva with species-specific monoclonal antibodies", Hybrid Hybridomics, 21, pp.225~232 (2002)