Monoclonal Antibody를 이용한 Streptococcus mutans 검출 방법의 임상적 적용에 관한 연구
DETECTION SYSTEM OF STREPTOCOCCUS MUTANS IN SALIVA USING MONOCLONAL ANTIBODY
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참고문헌
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초록
Monoclonal antibody를 이용한 Saliva-checkTM Mutans 키트의 타액 Streptococcus mutans 검출방법으로서의 활용도 와 임상적 우식지수 및 기존의 세균배양방법과의 상관관계를 알아보기 위해 2008년 2월에서 5월 중 평촌키즈웰치과에 내원 한 만 2세에서 만 8세 사이의 92명의 아동을 대상으로 Streptococcus mutans 검출검사를 실시하였으며 또한 우식에 영향 을 미치는 다른 요인인 치면세균막 pH와 타액완충능력검사를 실시하여 다음과 같은 결론을 얻었다. 1. Saliva-checkTM Mutans 검사결과 양성을 나타낸 아동은 27명으로 29.3%이었고, 음성을 나타낸 아동은 65명으로 70.65%이었다. 우식경험유치면률은 음성 아동 13.89%, 양성 아동 25.23% 으로 나타났다. 2. Monoclonal antibody를 이용한 검사방법인 Saliva-checkTM Mutans와 기존의 세균 배양방법인 Dentocult®-SM은 각 각 상이한 검사방법을 이용함에도 불구하고 검사결과 높은 상관관계를 보였다(p<0.01). 3. Saliva-checkTM Mutans 검사와 치면세균막 pH 검사와는 역상관관계를 나타내었으나(p<0.01), 타액완충능 검사와는 상관관계가 나타나지 않았다(p>0.05). 이상의 결과로 보았을 때 Monoclonal antibody를 이용한 검사방법인 Saliva-checkTM Mutans는 구강 내 Streptococcus mutans를 측정하는 방법으로 적당하며 또한 검사에 필요한 시간을 대폭 줄일 수 있고 사용방법도 매우 간편 하게 개발되어 환자들에게 적용함에 있어 효과적이라고 사료되었다.
Abstract
The purpose of this study was to evaluate the clinical effectiveness of new streptococcus detection system which used monoclonal antibody against Streptococcus mutans. 92 children aged between 2 and 8 were involved in this experiment and their saliva samples were collected for testing. Streptococcus mutans were measured by both monoclonal antibody-based detecting system(Salivacheck TMMutans) and dip slide detecting system(Dentocult-SM). The results showed that Saliva-checkTM Mutans levels had a significant correlation with dfs rate of subjects and the two test kits, Saliva-checkTM Mutans and Dentocult®-SM were shown to have a good correlation although they were based on different mechanism.
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