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Detection of Streptococcus mutans in human saliva and plaque using selective media, polymerase chain reaction, and monoclonal antibodies

김미아|Yong Jin Lee|Jae-Gon Kim|Jae-Hwan Kim
치의학연구원 (The Institute of Dental Science, Chosun University)|2019.06|Oral Biology Research|제43권 제2호|pp. 121-129|DOI https://doi.org/10.21851/obr.43.02.201906.121

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키워드

Streptococcus mutansSalivaDental plaqueAntibodies
주제분류치의학

초록

The objective of this study was to evaluate the distribution of dental caries-associated Streptococcus mutans in human saliva and plaque. The samples for this study were collected from 90 subjects (30 children, 30 adolescents, and 30 adults). The decayed, missing, and filled teeth (DMFT) and significant caries indices were evaluated. We applied polymerase chain reaction (PCR) analysis to detect S . mutans in each sample. Enumeration of S . mutans was conducted through culturing on Dentocult-SM and mitis salivarius agar medium. For saliva samples enzyme-linked immunosorbent assay (ELISA) analysis using monoclonal antibodies specific to antigen I/II and glucosyltransferase was done. We detected S . mutans in 79.7% and 56.8% of all saliva and plaque samples, respectively using PCR analyses. S . mutans were detected in 59.1%, 88.0%, and 88.9%, and in 86.4%, 56.0%, and 33.3% of saliva and plaque samples from children, adolescents, and adults, respectively. There were significantly higher levels of S . mutans in adolescents’ saliva more than any groups. There was a positive correlation between DMFT and the level of S . mutans reactivity measured using ELISA. Our results suggest that there should be more emphasis on adolescents’ oral hygiene more than in children or adults early prevention and research of dental caries.

Abstract

The objective of this study was to evaluate the distribution of dental caries-associated Streptococcus mutans in human saliva and plaque. The samples for this study were collected from 90 subjects (30 children, 30 adolescents, and 30 adults). The decayed, missing, and filled teeth (DMFT) and significant caries indices were evaluated. We applied polymerase chain reaction (PCR) analysis to detect S . mutans in each sample. Enumeration of S . mutans was conducted through culturing on Dentocult-SM and mitis salivarius agar medium. For saliva samples enzyme-linked immunosorbent assay (ELISA) analysis using monoclonal antibodies specific to antigen I/II and glucosyltransferase was done. We detected S . mutans in 79.7% and 56.8% of all saliva and plaque samples, respectively using PCR analyses. S . mutans were detected in 59.1%, 88.0%, and 88.9%, and in 86.4%, 56.0%, and 33.3% of saliva and plaque samples from children, adolescents, and adults, respectively. There were significantly higher levels of S . mutans in adolescents’ saliva more than any groups. There was a positive correlation between DMFT and the level of S . mutans reactivity measured using ELISA. Our results suggest that there should be more emphasis on adolescents’ oral hygiene more than in children or adults early prevention and research of dental caries.

참고문헌 (42)

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치아우식경험과 Streptococcus mutans의 관련성김설희, 서동원한국치위생학회 · 한국치위생학회지2012 · 제12권 제6호단일클론항체를 사용한 타액 내 Streptococcus mutans 검출김미아, 김재곤, 백병주, 양연미, 이경열, 이대우, 이민정대한소아치과학회 · 대한소아치과학회지2015 · 제42권 제1호단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정김미아, 김재곤, 김추성, 백병주, 양연미, 이경열, 임수민대한소아치과학회 · 대한소아치과학회지2010 · 제37권 제2호Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent박호원, 서현우, 이시영, 이정은, 이주현대한소아치과학회 · 대한소아치과학회지2018 · 제45권 제1호소아의 치면세균막에 존재하는 mutans streptococci의 분포국중기, 김화숙, 박종휘, 유소영, 이난영대한소아치과학회 · 대한소아치과학회지2004 · 제31권 제3호혼합치열기 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포도 조사박재홍, 이명성, 최성철대한소아치과학회 · 대한소아치과학회지2007 · 제34권 제2호

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