뱀장어(Anguilla japonica) 정자 활성에 따른 정액 분석 및 인공정장액 개선
Semen Analysis and Improvement of Artificial Seminal Plasma According to Sperm Activity in Eel Anguilla japonica
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study examined the characteristics of eel Anguilla japonica sperm using the CASA (computer-assisted sperm analysis) system and attempted to provide the composition for artificial seminal plasma by regulating of inorganic elements. Sperm samples were first divided into four groups based on motility and progressive motility: (A) 0–10%, (B) 10–40%, (C) 40–70%, and (D) 70–100%. For observing sperm velocity variations, VCL, which is curve motion velocity, showed the highest values in all groups. The directional factor, beat cross frequency, was lower in higher activity groups, showing an opposite correlation with sperm activity. The head sizes of spermatozoa in higher activity groups were significantly larger than those in lower activity groups. The Na+ and K+ ions were important in the inorganic composition of seminal plasma in this species. Furthermore, regulating the composition in artificial seminal plasma improved the formula compared to the existing element, exhibiting 120 mM Na and 30 mM K when the sperm was conserved for a long time and 120 mM NA and 40 mM K when the sperm was conserved for a short time.
Abstract
This study examined the characteristics of eel Anguilla japonica sperm using the CASA (computer-assisted sperm analysis) system and attempted to provide the composition for artificial seminal plasma by regulating of inorganic elements. Sperm samples were first divided into four groups based on motility and progressive motility: (A) 0–10%, (B) 10–40%, (C) 40–70%, and (D) 70–100%. For observing sperm velocity variations, VCL, which is curve motion velocity, showed the highest values in all groups. The directional factor, beat cross frequency, was lower in higher activity groups, showing an opposite correlation with sperm activity. The head sizes of spermatozoa in higher activity groups were significantly larger than those in lower activity groups. The Na+ and K+ ions were important in the inorganic composition of seminal plasma in this species. Furthermore, regulating the composition in artificial seminal plasma improved the formula compared to the existing element, exhibiting 120 mM Na and 30 mM K when the sperm was conserved for a long time and 120 mM NA and 40 mM K when the sperm was conserved for a short time.
참고문헌 (34)
- J. F. Asturiano, "Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm", Fish Physiology and Biochemistry, 30(3-4), pp.283~293 (2004), Springer Science and Business Media LLC[DOI]
- Roland Billard, "Some problems related to the assessment of sperm motility in freshwater fish", Journal of Experimental Zoology, 261(2), pp.122~131 (1992), Wiley[DOI]
- Yusuf Bozkurt, "Seminal plasma composition and its relationship with physical spermatological parameters grass carp (Ctenopharyngodon idella) semen: With emphasis on sperm motility", Aquaculture Research, 39, pp.1666~1672 (2008), Hindawi Limited[DOI]
- Bozkurt Y, "Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma(Dumeril, 1858)semen : With emphasis on sperm motility", Czech J Anim Sci, 56, pp.355~364 (2011)
- Beata Irena Cejko, "Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.", Fish Physiology and Biochemistry, 44(6), pp.1435~1442 (2018), Springer Science and Business Media LLC[DOI]
- K. Dziewulska, "Sperm motility characteristics of wild Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta m. trutta L.) as a basis for milt selection", Journal of Applied Ichthyology, 27(4), pp.1047~1051 (2011), Hindawi Limited[DOI]
- M. Hidiroglou, "Zinc in Mammalian Sperm: A Review", Journal of Dairy Science, 67(6), pp.1147~1156 (1984), American Dairy Science Association[DOI]
- Manuel Hidalgo, "Influence of staining and sampling procedures on goat sperm morphometry using the Sperm Class Analyzer", Theriogenology, 66(4), pp.996~1003 (2006), Elsevier BV[DOI]
- 정민환, "담수순화 감성돔 Acanthopagrus schlegelii의 정액특성 및 정자운동성", 발생과 생식, 15(2), pp.151~158 (2011), 한국발생생물학회
- 정민환, "묵납자루(Acheilognathus signifer) 정자의 냉동보존", 한국수산과학회지, 47(1), pp.39~44 (2014), 한국수산과학회[DOI]
- S. Judycka, "The effect of supplementation of a trehalose-based extender with KCl on rainbow trout (Oncorhynchus mykiss) sperm freezability and post-thaw motility", Aquaculture, 465, pp.303~310 (2016), Elsevier BV[DOI]
- Hirohiko Kagawa, "Effects of rearing period in seawater on induced maturation in female Japanese eel Anguilla japonica", Fisheries science, 64(1), pp.77~82 (1998), Japanese Society of Fisheries Science[DOI]
- D. E. Kime, "Unusual motility characteristics of sperm of the spotted wolffish", Journal of Fish Biology, 61(6), pp.1549~1559 (2002), Wiley[DOI]
- Kobayashi T, "Improvement of sperm motility of sex-reversed male rainbow trout, Oncorhynchus mykiss, by incubation in high-pH artificial seminal plasma", Environment Biol Fish, 69, pp.419~425 (2004)
- F Lahnsteiner, "Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism", Aquaculture, 163(1-2), pp.163~181 (1998), Elsevier BV[DOI]
- 레민황, "말쥐치(Thamnaconus modestus) 정액의 특성과 정자의 미세구조", 발생과 생식, 11(3), pp.227~233 (2007), 한국발생생물학회
- Otomar Linhart, "Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos", Cryobiology, 41(3), pp.241~250 (2000), Elsevier BV[DOI]
- M. Morisawa, "Effects of Osmolality and Potassium on Motility of Spermatozoa From Freshwater Cyprinid Fishes", Journal of Experimental Biology, 107(1), pp.95~103 (1983), The Company of Biologists[DOI]
- M. Morisawa, "Adaptation and strategy for fertilization in the sperm of teleost fish", Journal of Applied Ichthyology, 24(4), pp.362~370 (2008), Hindawi Limited[DOI]
- T Miura, "Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica)", Proceedings of the National Academy of Sciences, 88(13), pp.5774~5778 (1991), Proceedings of the National Academy of Sciences[DOI]
- Akira Miura, "Administration of 17α-hydroxyprogesterone into mature male Japanese eel reduces sperm motility by decreasing potassium ion concentrations in the seminal plasma", Aquaculture, 414-415, pp.217~223 (2013), Elsevier BV[DOI]
- Hiromi Ohta, "Diluent for cool storage of the Japanese eel (Anguilla japonica) spermatozoa", Aquaculture, 142(1-2), pp.107~118 (1996), Elsevier BV[DOI]
- Hiromi Ohta, "Changes in fertilization and hatching rates with time after ovulation induced by 17, 20β-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica", Aquaculture, 139(3-4), pp.291~301 (1996), Elsevier BV[DOI]
- Hiromi Ohta, "Increases in concentrations of potassium and bicarbonate ions promote acquisition of motility in vitro by Japanese eel spermatozoa", The Journal of Experimental Zoology, 277(2), pp.171~180 (1997), Wiley[DOI]
- H Ohta, "Control by the environmental concentration of ions of the potential for motility in Japanese eel spermatozoa", Aquaculture, 198(3-4), pp.339~351 (2001), Elsevier BV[DOI]
- DS Peñaranda, "European Eel Sperm Diluent for Short‐term Storage", Reproduction in Domestic Animals, 45(3), pp.407~415 (2010), Wiley[DOI]
- E Rurangwa, "The measurement of sperm motility and factors affecting sperm quality in cultured fish", Aquaculture, 234(1-4), pp.1~28 (2004), Elsevier BV[DOI]
- 사수진, "수퇘지 정자의 운동성,생존성 및 체외수정 능력에 대한 시판 액상 정액 보존액과 보존 기간의 영향", Reproductive & Developmental biology, 35(3), pp.203~207 (2011), 사단법인 한국동물생명공학회
- Hiroyuki Takai, "Change in intracellular K+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts", Journal of Cell Science, 108(3), pp.1175~1181 (1995), The Company of Biologists[DOI]
- Edward A. Trippel, "Fertility and sperm quality of virgin and repeat-spawning Atlantic cod (Gadus morhua)and associated hatching success", Canadian Journal of Fisheries and Aquatic Sciences, 49(10), pp.2118~2127 (1992), Canadian Science Publishing[DOI]
- V. M. Tuset, "Sperm morphology and its influence on swimming speed in Atlantic cod", Journal of Applied Ichthyology, 24(4), pp.398~405 (2008), Hindawi Limited[DOI]
- V. M. Tuset, "Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa", Journal of Applied Ichthyology, 24(4), pp.393~397 (2008), Hindawi Limited[DOI]
- Mariola Wojtczak, "Polymorphism of transferrin of carp seminal plasma: Relationship to blood transferrin and sperm motility characteristics", Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 148(4), pp.426~431 (2007), Elsevier BV[DOI]
- Kiichiro YAMAMOTO, "Artificial Maturation of Cultivated Male Japanese Eels by Synahorin Injection", NIPPON SUISAN GAKKAISHI, 38(10), pp.1083~1090 (1972), Japanese Society of Fisheries Science[DOI]