본문 바로가기
KERD
논문KCI 등재

Effects of Cryoprotectants and Diluents on the Cryopreservation of Spermatozoa from Far Eastern Catfish, Silurus asotus

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류생물학

초록

The aim of this study was to compare the efficacy of cryopreservation methods for ex situ conservation of spermatozoa from far eastern catfish, Silurus asotus. The spermatozoa activity index (SAI) and hatching rates were higher in spermatozoa stored in Alserver’s solution than those of spermatozoa stored in glucose solution. The SAI and hatching rates in all experimental groups gradually decreased with increasing duration of storage. Additionally, the SAI and hatching rates gradually decreased with increasing thawing temperatures at all storage durations (P<0.05). Based on the SAI and hatching rates, our results suggest that the optimal cryopreservation conditions of catfish spermatozoa involve storage in Alserver’s solution with 15% ethylene glycol, and thawing at 25℃. Cryopreservation of spermatozoa is a useful and reliable technique for conserving gene resources and for artificial propagation of far eastern catfish.

Abstract

The aim of this study was to compare the efficacy of cryopreservation methods for ex situ conservation of spermatozoa from far eastern catfish, Silurus asotus. The spermatozoa activity index (SAI) and hatching rates were higher in spermatozoa stored in Alserver’s solution than those of spermatozoa stored in glucose solution. The SAI and hatching rates in all experimental groups gradually decreased with increasing duration of storage. Additionally, the SAI and hatching rates gradually decreased with increasing thawing temperatures at all storage durations (P<0.05). Based on the SAI and hatching rates, our results suggest that the optimal cryopreservation conditions of catfish spermatozoa involve storage in Alserver’s solution with 15% ethylene glycol, and thawing at 25℃. Cryopreservation of spermatozoa is a useful and reliable technique for conserving gene resources and for artificial propagation of far eastern catfish.

참고문헌 (31)

  1. Bart AN, "Cryopreservation of blue catfish spermatozoa and subsequent fertilization of channel catfish eggs", Trans Amer Fish Soc, 127, pp.819~824 (1998)
  2. Carolsfeldt J, "Cryopreservation of sperm in Brazilian migratory fish conservation", J Fish Biol, 63, pp.472~489 (2003)
  3. Chang Y-J, "Cryopreservation of tiger puffer(Takifugu rubripes)sperm", Dev Reprod, 1, pp.29~36 (1997)
  4. Choi GC, "Induced breeding and indoor culture of the catfish(Silurus asotus)(Teleostomi : Siluridae)", J Aquacult, 5, pp.117~126 (1992)
  5. Christensen JM, "Cryopreservation of channel catfish spermatozoa: effect of cryoprotectant, straw size and formulation of extender", Theriogenology, 47, pp.639~645 (1997)
  6. Christensen JM, "Cryopreservation of channel catfish sperm: effects of cryoprotectant exposure time, cooling rate, thawing conditions, and maleto-male variation", Theriogenology, 63, pp.2103~2112 (2005)
  7. Conget P, "Cryopreservation of rainbow trout(Oncorhynchus mykiss)spermatozoa using programmable freezing", Aquaculture, 143, pp.319~329 (1996)
  8. Correa JR, "Preparation and recovery of frozen-thawed bovine spermatozoa via various sperm selection techniques employed in assisted reproductive technologies", Theriogenology, 46, pp.1225~1232 (1996)
  9. Guest WC, "Preservation of channel catfish sperm", Trans Amer Fish Soc, 3, pp.469~474 (1976)
  10. Horvath A, "The effect of cryoprotectants on the motility and fertilizing capacity of cryopreserved African catfish, Clarias gariepinus (Burchell, 1822) sperm", Aquacult Res, 31, pp.317~324 (2000)
  11. Kim DS, "Cytogenetic traits and gonad development of induced triploidy in far eastern catfish(Silurus asotus)", Gene &Genomics, 23, pp.55~62 (2001)
  12. Kim DS, "Cytogenetic analysis of catfish(Silurus asotus)(Teleostomi : Siluriformes)", Ocean Polar Res, 10, pp.33~37 (1988)
  13. Kwantong S, "Effect of cryoprotectants, extenders and freezing rates on the fertilization rate of frozen striped catfish, Pangasius hypophtalmus (Sauvage), sperm", Aquacult Res, 34, pp.887~893 (2003)
  14. Lahnsteiner F, "Fine structure changes in spermatozoa of the grayling, Thymallus thymallus (Pisces: Teleostei), during routine cryopreservation", Aquaculture, 103, pp.73~84 (1992)
  15. Lang RP, "The use of dairy protocols for sperm cryopreservation of blue catfish, Ictalurus furcatus", J World Aquacult Soc, 34, pp.66~75 (2003)
  16. Linhart O, "Cryopreservation of European catfish (Silurus glanis L.) spermatozoa", Aquaculture, 115, pp.347~359 (1993)
  17. Linhart O, "Optimalization of artificial propagation in European catfish, Silurus glanis L", Aquaculture, 235, pp.619~632 (2004)
  18. Linhart O, "Increased rate of egg fertilization in artificial propagation of sheatfish (Silurus glanis L.) by means of suppressing the movements of spermatozoa with immobilization solution", Aquaculture, 65, pp.353~358 (1987)
  19. Linhart O, "Cryopreservation of European catfish Silurus glanis sperm: sperm motility, viability, and hatching success of embryos", Cryobiology, 51, pp.250~261 (2005)
  20. Marian T, "Selection Hybridization and Genetic Engineering in Aquaculture Volume I", pp.1987, FAO
  21. Muchlisin ZA, "Influence of cryoprotectants on abnormality and motility of baung(Mystus nemurus)spermatozoa after long-term cryopreservation", Cryobiology, 58, pp.166~169 (2009)
  22. Pickett BW, "Preservation of bovine spermatozoa by freezing in straws : a review", J Dairy Sci, 57, pp.1287~1301 (1974)
  23. Rurangwa E, "Quality control of refrigerated and cryopreserved semen using computer-assisted sperm analysis, viable staining and standardized fertilization in African catfish (Clarias gariepinus)", Theriogenology, 55, pp.751~769 (2001)
  24. Steyn GJ, "The effect of freezing rate on the survival of cryopreserved African sharptooth catfish(Clarias gariepinus)spermatozoa", Cryobiology, 30, pp.581~590 (1993)
  25. Steyn GJ, "Preliminary investigations on the cryopreservation of Clarias gariepinus (Clariidae: pisces) sperm", Water SA, 11, pp.15~18 (1985)
  26. Strüssmann CA, "Morility of pejerrey, Odontesthes bonariensis spermatozoa", Fish Sci, 60, pp.9~13 (1994)
  27. Taddei AR, "Is cryopreservation a homogenous process? Ultrastructure and motility of untreated, prefreezing, and postthawed spermatozoa of Diplodus puntazzo Cetti", Cryobiology, 42, pp.44~255 (2001)
  28. Tiersch TR, "Cryopreservation of channel catfish sperm-storage in cryoprotectants, fertilization trials, and growth of channel catfish produced with cryopreserved sperm", Trans Amer Fish Soc, 123, pp.580~586 (1994)
  29. Urbanyi B, "Cryopreservation of African catfish (Clarias gariepinus) sperm using different cryoptotectants", Theriogenology, 51, pp.296~ (1999)
  30. Viveiros ATM, "Influence of cooling rates and plunging temperatures in an interrupted slow-freezing procedure for African catfish, Clarias gariepinus", Cryobiology, 43, pp.276~287 (2001)
  31. 양원석, "Identification of Skeletal Deformities in Far Eastern Catfish, Silurus asotus under Indoor Aquaculture Condition", 발생과 생식, 19(3), pp.153~161 (2015), 한국발생생물학회

관련 논문AI추천

이 간행물의 다른 논문 (10건)