산천어 (Oncorhynchus masou masou) 정자의 냉동보존에 미치는 희석액과 동해방지제의 영향
Effects of Diluents and Cryoprotectants on Sperm Cryopreservation in Masou Salmon, Oncorhynchus masou masou
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참고문헌
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초록
We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained 18±1 mmol/L potassium, 144±4mmol/L sodium, 116±3mmol/L chloride, 83.2±3.1mg/L calcium, 14.8±0.7mg/L magnesium, 45±9mg/L glucose, and 1.0±0.0g/L total protein. The osmolality and pH of seminal plasma were 287±7 and 7.7±0.1mmol/kg, respectively, and the spermatocrit was 28±2. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.
Abstract
We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained 18±1 mmol/L potassium, 144±4mmol/L sodium, 116±3mmol/L chloride, 83.2±3.1mg/L calcium, 14.8±0.7mg/L magnesium, 45±9mg/L glucose, and 1.0±0.0g/L total protein. The osmolality and pH of seminal plasma were 287±7 and 7.7±0.1mmol/kg, respectively, and the spermatocrit was 28±2. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.
참고문헌 (22)
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