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말쥐치(Thamnaconus modestus) 정액의 특성과 정자의 미세구조

Milt Properties and Spermatozoa Structure of Filefish (Thamnaconus modestus)

장영진|김성연|임한규|민병화|레민황
한국발생생물학회 (The Korea Society Of Developmental Biology)|2007.12|발생과 생식 (DEVELOPMENT & REPRODUCTION)|제11권 제3호|pp. 227-233

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참고문헌

주제분류생물학

초록

말쥐치(Thamnaconus modestus) 정액의 물리화학적 특성과 정자의 미세구조에 대하여 분석하였다. 정액량과 정자농도, spermatocrit는 각각 0.3±0.1 mLfish-1, 2.6±0.1x107 spermatozoamL-1, 73.3±6.7였다. 정장의 화학적 조성에 있어서 potassium 9.8±0.9 mmol·L-1, sodium 164.0±4.0 mmol·L-1, chloride 151.0±1.2 mmol·L-1, calcium 14.9±0.6 mg·dL-1, magnesium 7.2±0.1 mg·dL-1, glucose 1.0 mg·dL-1, 총단백질 0.1 g·dL-1, 총지질 1.0 mg·dL-1 였으며, 삼투농도와 pH는 각각 322.8±2.8 mmolkg-1, 7.7±0.1였다. 투과형 전자현미경으로 미세구조를 관찰한 결과, 정자는 첨체가 없는 머리, 5개의 미토콘드리아로 이루어진 중편부 및 “9+2”의 미세소관 편모를 가진 꼬리로 구성되어 있었다. 세로로 절단한 정자 머리는 장축 1.3-1.6 m, 단축1.0-1.3 m로 말편자 모양을 하고 있었다.

Abstract

The milt properties of filefish (Thamnaconus modestus) included physical properties of sperm and biochemical properties of seminal plasma. The physical properties of milt were 0.3±0.1 mLfish-1 in sperm volume, 2.6±0.1x107 spermatozoamL-1 in sperm concentration and 73.3±6.7 in spermatocrit. The biochemical properties of seminal plasma contained 9.8±0.9 mmol·L-1 potassium, 164.0±4.0 mmol·L-1 sodium, 151.0±1.2 mmol·L-1 chloride, 14.9±0.6 mg·dL-1 calcium, 7.2±0.1 mg·dL-1 magnesium, 1.0 mg·dL-1 glucose, 0.1 g·dL-1 total protein and 1.0 mg·dL-1 total lipid. The osmolality and pH of seminal plasma were 322.8±2.8 mmolkg-1 and 7.7±0.1, respectively. Using transmission electron microscope, the spermatozoon of filefish consisted of three parts: head without acrosome, mid-piece with five mitochondria and flagellum with 9+2 pattern. The head of spermatozoon in longitudinal section was horseshoe-shaped, and 1.3-1.6 m long and 1.0-1.3 m wide.

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